siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance efflux pump gene acrA, and preparation method thereof

A nano and nano-carrier technology, applied in the field of siRNA-DNA nano system, can solve the problems of short effective time, expensive reagents, long experiment period, etc.
CN107805643AActive Publication Date: 2018-03-16SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SICHUAN UNIV
Publication Date
2018-03-16

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Abstract

The invention belongs to the technical field of research on the drug resistance of microorganisms, and provides a siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance effluxpump gene acrA, and a preparation method thereof, specifically a DNA nanometer vector, which has a tetrahedron-like structure formed by assembling an oligonucleotide A single chain, an oligonucleotide B single chain, and an oligonucleotide C single chain. Based on the DNA nanometer vector, the present invention further provides a nanometer system, a preparation method and applications thereof, wherein the DNA nanometer vector loads a siRNA targeting efflux pump gene to from the nanometer system. According to the present invention, the DNA nanometer vector has the tetrahedron-like structure, can stably load siRNA, and can safely, rapidly and efficiently transport the siRNA to target cells so as to inhibit the expression of the Salmonella efflux pump gene, such that the sensitivity on antibiotics by Salmonella is improved so as to effectively inhibit the drug resistance of Salmonella.
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Description

technical field

[0001] The invention belongs to the technical field of microbial drug resistance research, and in particular relates to a DNA nanocarrier, an siRNA-DNA nanosystem for targeting and inhibiting Salmonella drug resistance efflux pump gene acrA, a preparation method and application thereof. Background technique

[0002] Salmonella is a very important class of Gram-negative bacteria that can cause zoonotic diseases. The problem of Gram-negative bacteria, especially Salmonella antibiotic resistance is increasing, which has become one of the hot spots of global concern. The failure of antibiotic treatment of bacterial infections is increasing due to the continuous spread of multi-drug resistant Gram-negative bacteria. The World Health Organization (WHO) declares that drug-resistant bacteria have become one of the main factors threatening human health, making it more urgent and meaningful to find ways to solve bacterial resistance. Studies have shown that efflux pu...

Claims

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