Primer for amplifying Bulaikaite black cattle UCK2 gene and kit and method for screening high-quality cattle breed of Bulaikaite black cattle

Abstract

The invention discloses a primer for amplifying a Bulaikaite black cattle UCK2 gene and a kit and a method for screening a high-quality cattle breed of Bulaikaite black cattle. The method comprises the following step: selecting a cattle breed of Bulaikaite black cattle, which has a high mRNA (messenger Ribonucleic Acid) expression level of the Bulaikaite black cattle UCK2 gene, as the high-qualitycattle breed. The invention provides the method for screening the high-quality cattle breed by detecting the mRNA expression level of the UCK2 gene for the first time, and a new idea is provided forcultivating a high-quality Bulaikaite beef cattle variety. In a detection process, the specific primer and the kit, disclosed by the invention, are utilized; the expression amount of the mRNA level ofthe Bulaikaite black cattle is detected by utilizing a qRT-PCR (quantitative Reverse Transcription-Polymerase Chain Reaction) method; a protection expression level is judged through the mRNA expression level, and the high-quality cattle breed can be screened; a transcriptome sequencing technology is combined with a qRT-PCR technology, so that the instability of traditional SNP (Single NucleotidePolymorphism) and the limitation of common RFLP (Restricted Fragment Length Polymorphism) are overcome; the primer, the kit and the method have the advantages of simplicity, rapidness, low cost, highprecision, direct detection result and convenience for popularization.

Description

technical field [0001] The invention relates to a primer used for amplifying the UCK2 gene of Black Black cattle, and also relates to a kit and a screening method for screening high-quality Black Black cattle breeds, belonging to the technical field of animal molecular genetics. Background technique [0002] With the improvement of living standards and the development of food culture, people's choice of meat is no longer limited to traditional pork, chicken and other products. The amino acid composition in beef is closer to the human body level than pork, making it more in line with human health dietary needs. At present, my country's per capita beef consumption is only about 5 kilograms, while the world average is above 10 kilograms, and the per capita consumption of developed countries is as high as 50 kilograms, so my country's beef demand still has a lot of room for growth. my country has a very rich variety of local beef cattle, such as Qinchuan cattle and Luxi yellow ...

AI Technical Summary

Problems solved by technology

[0004] Nowadays, there are few reports on the methods of screening or cultivating high-quality black black cattle breeds. At present, SSCP and common RFLP techniques are commonly used for single nucleotide polymorphism detection and population genetic polymorphism analysis. These methods Unstable, under normal conditions, the mutation may not be detected, therefore, a considerable proportion of false negative results may be encountered in practical applications; at the same time, the sensitivity of the current detection technology is not high, and it is affected by many factors, resulting in repeated Poor performance; coupled with its complex operation process, which greatly limits its application in production

Images