Method for sensing and analyzing concentration of ascorbic acid online based on optical microscope and auxiliary color developing agent

A technology of optical microscopy and ascorbic acid, which is applied in the field of nanomaterials and analytical chemistry, can solve problems such as more environmental light requirements, and achieve the effects of easy-to-obtain raw materials, simple preparation, and real-time continuous analysis

Active Publication Date: 2018-04-06
CAPITAL NORMAL UNIVERSITY
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this work can sensitively detect physiologically active substances, it is based on the principle of fluorescence luminescence, which re

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for sensing and analyzing concentration of ascorbic acid online based on optical microscope and auxiliary color developing agent
  • Method for sensing and analyzing concentration of ascorbic acid online based on optical microscope and auxiliary color developing agent
  • Method for sensing and analyzing concentration of ascorbic acid online based on optical microscope and auxiliary color developing agent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1: Preparation of color developer

[0039] (1) Preparation of cobalt oxyhydroxide suspension: first, add 10 mM CoCl 2 Mix 1mL of the solution with 250μL of 1.0M NaOH solution and sonicate for 1 minute; add 50μL of 0.9M NaClO solution and sonicate for 10 minutes, and finally make the volume to 40ml to obtain a 40μg / mL CoOOH suspension for use.

[0040] (2) Preparation of 3,3',5,5'-tetramethylbenzidine solution: Disperse 10mg TMB in 1 mL of DMSO, add 1 mL of 0.1 mM CA solution and 20 μL of 0.5 M EDTA solution, and dilute to volume. To 40mL, 2mM TMB solution is obtained.

[0041] (3) Mix the above-mentioned TMB solution (2mM) and CoOOH suspension (40μg / mL) in equal volume ratios. After mixing thoroughly, this TMB-CoOOH system can be used as a developer for detecting ascorbic acid and placed in the refrigerator. spare.

Example Embodiment

[0042] Example 2

[0043] Using the developer provided in Example 1, ascorbic acid was monitored in an optical online system. Specific steps are as follows:

[0044] (1) The developer provided in Example 1 and deionized water were continuously passed into a transparent capillary with an inner diameter of 250nm fixed under an optical microscope at a speed of 3μL / min, and then used an optical microscope (objective lens 40X, Manual zoom 1.6X, black-and-white mode) Take 30 pictures of the transparent capillary at equal time intervals continuously within 150s, and use the image analysis software ImageJ to convert the light intensity signals of the 30 pictures obtained continuously into 30 digital signals. The average value, as the light intensity value I 0 ;

[0045] (2) After 20 minutes of deionized water was introduced, five ascorbic acid standards with concentrations of 1 μM, 5 μM, 10 μM, 20 μM and 50 μM were successively passed into the chromogenic reagent at a rate of 3 μL / min. In...

Example Embodiment

[0051] Experimental example 1: The influence of different shooting modes on the sensitivity of detecting ascorbic acid

[0052] The color and black and white shooting modes in the microscope will affect the subsequent image recognition results. Therefore, we designed ascorbic acid recognition experiments in different shooting modes to select the optimal shooting mode; the four shooting modes are specifically: color shooting mode under 20X objective lens, 40X objective lens and color under overall manual magnification 1.6X Shooting mode, black and white shooting mode under 20X objective lens, 40X objective lens and black and white shooting mode under overall manual magnification 1.6X. After mixing the developer with different concentrations of ascorbic acid in a centrifuge tube at a volume ratio of 10:2, use a syringe to manually pass the reaction system into the capillary, and take pictures under the microscope, where the concentration of ascorbic acid is 1.5 , 10, 20, 50μM. Un...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for sensing and analyzing the concentration of ascorbic acid online based on an optical microscope and an auxiliary color developing agent and relates to a color developing agent for assisting the online sensing and analysis of the concentration of ascorbic acid through the optical microscope. The color developing agent is a liquid color developing agent and is prepared by mixing hydroxy cobalt oxide and 3,3',5,5'-tetramethyl benzidine. The invention simultaneously provides the method for sensing and analyzing the concentration of ascorbic acid online based on the optical microscope. According to the method, a picture of a mixture of the color developing agent in a transparent capillary tube and ascorbic acids of different concentrations is taken by virtue of the microscope, image signals are converted into digital signals which are in a certain linear relation with the concentrations of ascorbic acids by virtue of software, and then the concentration detection of ascorbic acids is realized. The color developing agent is easy and convenient to prepare, can be used for rapidly and continuously analyzing the change condition of ascorbic acid in real time, is capable of monitoring the change condition of ascorbic acid in the physiological process of cranial nerves in a living body and has certain guiding significance to the establishment of other optical sensing system methods, and the raw materials are easily available.

Description

technical field [0001] The invention belongs to the field of nanometer materials and analytical chemistry, and relates to an optical detection method of ascorbic acid. Background technique [0002] Ascorbic acid is a water-soluble six-carbon sugar acid and an important neuromodulator with two dissociated protons (pKa=4.04, 11.34) under physiological conditions. Its electron-donating properties make it a famous small molecule antioxidant and free radical scavenger in cells, and some diseases are closely related to the level of ascorbic acid. Therefore, it is of practical significance to develop a sensing method for real-time monitoring of ascorbic acid changes in physiological and pathological processes. [0003] At present, there are many methods for the determination of the content of ascorbic acid using electrochemical detection of ascorbic acid, such as potentiostatic analysis, differential pulse voltammetry, and cyclic voltammetry. In addition, there are mainly high-pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78G01N21/84
CPCG01N21/78G01N21/8483G01N2021/775
Inventor 林雨青王超丁永奇
Owner CAPITAL NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap