A hollow-shaped platinum-copper-cobalt ternary alloy nanoparticle mimic enzyme and its preparation and application
A nanoparticle, cobalt alloy technology, applied in the field of catalytic materials and analytical chemistry, can solve the problems of complex synthesis and purification steps, limit the application of natural enzymes, inactivation and denaturation, etc., and achieve high chemical stability, high repeatability, and non-toxic reagents. Effect
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Embodiment 1
[0035] Preparation of platinum-copper-cobalt alloy nanoparticle mimic enzyme:
[0036] co 3 o 4 Preparation of particles: Put 0.6g cobalt acetate, 2.2g CTAB, 60ml ethylene glycol, and 11ml water in a 100ml beaker, stir magnetically for 20 minutes, then transfer the mixture to a 100ml polytetrafluoroethylene reactor at 180°C for 48 hours. The obtained black precipitate was washed twice with water and ethanol respectively, and then vacuum-dried at 60°C to obtain a black solid powder, which was calcined at 320°C for 10 minutes to obtain black Co 3 o 4 particles.
[0037] Take PVP, glycine, gCo 3 o 4 、K 2 PtCl 4 , CuCl 2 、NiCl 2 , mixed with deionized water and ultrasonically dispersed for 5 minutes, and the mixed solution was transferred to a 20mL reactor at 200°C for 6 hours. After the reaction, the product was washed twice with ethanol and water respectively, and centrifuged at 10,000 rpm / min for 15-20 min. and finally dispersing the obtained alloy nanoparticles into...
Embodiment 2
[0046] The method for detecting glucose concentration, the process is as follows:
[0047] 1) Mix the platinum-copper-cobalt alloy nanoparticle mimetic enzyme prepared in Example 1 with the TMB solution, and record the absorbance of the characteristic absorption peak at 650 nm using the absorption curve of the solution with an ultraviolet-visible absorption spectrometer.
[0048] 2) Take 3.0ml of deionized water, add 200μl of 1mg / ml glucose oxidase (GOX), 0-400μl of 0.1M glucose aqueous solution to it in sequence, and then add 20μl of 20mM 3,3',5,5'- Tetramethylbenzidine (TMB), 5 μl of 1.3 mM platinum-copper-cobalt alloy nanoparticle simulated enzyme solution, and then mix the above solution evenly, and place it at room temperature (25° C.) for 15 minutes, and use a UV-visible spectrophotometer to detect the absorbance value. Draw a working curve by plotting the detected absorbance value at 650nm with the added glucose concentration.
[0049] Colorimetric detection of glucose...
Embodiment 3
[0053] The method for detecting the concentration of the hanging white block is as follows:
[0054] 1) Mix the platinum-copper-cobalt alloy nanoparticle simulant enzyme prepared in Example 2 with hydrogen peroxide and TMB solution, use a UV-visible absorption spectrometer to scan the kinetic mode to record the absorption curve of the solution every 2 minutes, and record the characteristic absorption at 650nm The absorbance of the peak is used to monitor and calculate the oxidation rate of TMB through the change of absorbance with time.
[0055] 2) After mixing the platinum-copper-cobalt alloy nanoparticle mimic enzyme prepared in Example 2 with hydrogen peroxide and TMB solution, add 0.3, 0.5, 1.0, 2.0, 5.0, 10.0, 15.0, 20.0, 30.0, 50.0, 60.0 , 80.0, and 100 μM of the hanging white block solution, use the UV-visible absorption spectrometer to scan the kinetic mode to record the absorption curve of the solution every 2 minutes, record the absorbance of the characteristic absor...
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