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Method for producing gamma-polyglutamic acid by using Flammulina velutipes waste

A technology of polyglutamic acid and Flammulina velutipes is applied in the field of green biological materials to achieve the effects of less heat consumption, lower production cost and shorter production cycle

Inactive Publication Date: 2018-04-20
镇江市天益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for producing gamma-polyglutamic acid by using Flammulina velutipes waste

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Embodiment 1

[0024] The main material of the waste culture medium is Flammulina velutipes mycelium, and 90 kg of remaining culture components composed of cottonseed hulls, bran, corn cobs, bean cakes, and sawdust. After adding 12 kg of bean cake powder, it is sterilized by a known sterilization method. Spread flat in the fermentation tray,

[0025] The inoculation temperature is 20-85℃, and the best condition is 80-85℃; the inoculation of bioengineering bacteria-Bacillus subtilis, the inoculation amount is 10 kg, mixed with 140 kg of sterilized water before use. Therefore, the specific method is: add 500 grams to each fermentation tray, spray 750 ml evenly, and inoculate a mixture of bacteria and sterilized water. Fermentation pallet truck 12 trays / unit, double door 24 trays, each fermentation box contains 12 kg of raw materials and enters the management. The fermentation temperature is 38-40°C, and the solid fermentation time of Bacillus subtilis is 24-48 hours. According to the inspection...

Embodiment 2

[0028] The main material of the waste culture medium is Flammulina velutipes mycelium, as well as 180 kg of remaining culture components composed of cottonseed hulls, bran, corn cob, bean cake, and sawdust. After adding 25 kg of bean cake powder, it is sterilized by known sterilization methods. Spread flat in the fermentation tray,

[0029] The inoculation temperature is 20-85℃, and the best condition is 80-85℃; the inoculation of bioengineering bacteria-Bacillus subtilis, the inoculation amount is 20 kg, mixed with 280 kg of sterilized water before use. Therefore, the specific method is: add 500 grams to each fermentation plate, spray 750 milliliters evenly, and inoculate a mixture of bacteria and sterilized water. Fermentation pallet truck 12 trays / unit, double door 24 trays, each fermentation box contains 12 kg of raw materials and enters the management. The fermentation temperature is 38-40°C, and the solid fermentation time of Bacillus subtilis is 40 hours. According to th...

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Abstract

The invention provides a method for producing gamma-polyglutamic acid by using Flammulina velutipes waste, and relates to the agricultural technology and the technical field of fungal solid culture material reuse. According to the present invention, Bacillus subtilis is inoculated in Flammulina velutipes waste, such that common feeds or live bacteria feeds can be produced, and the effective component gamma-polyglutamic acid (gamma-PGA) can be produced, wherein gamma-polyglutamic acid (gamma-PGA) is a novel environmentally-friendly biomaterial, has characteristics of no harm to the environment,edibility, no toxicity, no environment pollution, excellent biodegradability, excellent film forming property, excellent fiber formation, excellent plasticity, excellent adhesion, excellent moistureretention and other unique physicochemical and biological characteristics, and is the environmentally-friendly biological product, the large-scale extraction of gamma-polyglutamic acid (gamma-PGA) through the Flammulina velutipes waste is not reported, and the functional material gamma-polyglutamic acid (gamma-PGA) is favored by people, is gradually used in pharmaceutical manufacturing, food processing, and anti-freezing and preservation of vegetables, fruits and seafood, and can further be used in cosmetics industry, tobacco, leather manufacturing, plant seed protection and other fields; andwith the method, the new method for the bio-utilization of Flammulina velutipes waste is expanded, and the method has great development value and broad prospect.

Description

Technical field [0001] The invention relates to the technical field of agricultural technology and the reuse of fungal solid culture materials, especially the technical field of the treatment and processing of solid waste materials of Flammulina velutipes, and the production method of the non-toxic, moisturizing, new-type green biological material-polyglutamic acid for agricultural use . Background technique [0002] Flammulina velutipes waste (bacterial residue) is treated by bioengineering bacteria, which can reduce the environmental pollution of waste. Since the Flammulina velutipes waste contains protein, amino acids, sugars, vitamins and cellulose, it can be fermented by bioengineered bacteria to produce animal feed, active bacteria fertilizer and new products of environmental engineering bacteria. The 1 million bottles of Flammulina velutipes produced in Jiangsu and Zhejiang are not fully utilized. If the waste material of Flammulina velutipes is used to inoculate the bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/125
CPCC12P13/005
Inventor 陈顺志王林王信超
Owner 镇江市天益生物科技有限公司
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