Micro-fluidic electrical impedance detecting and sorting chip for Caenorhabditis elegans, and system and method thereof

Abstract

The invention discloses a micro-fluidic electrical impedance detecting and sorting chip for Caenorhabditis elegans. The chip comprises a substrate, a micro-fluidic channel layer and a gas cavity layerwhich are sequentially stacked from bottom to top, a pair of spaced coplanar microelectrodes is arranged on the substrate, the micro-fluidic channel layer comprises a nematode micro-channel positioned at the upstream, a target nematode channel and a remaining nematode channel which are positioned at the bifurcated downstream, a lower nematode suspension inlet, a lower target nematode sample outlet and a lower remaining nematode sample outlet, and the gas cavity layer comprises a target cavity, a remaining cavity, an upper nematode suspension inlet, an upper target nematode sample outlet and an upper remaining nematode sample outlet. The coplanar microelectrodes are arranged in the micro-fluidic electrical impedance detecting and sorting chip to detect nematode samples to be sorted, and the on-off of a compressed air introduced to the target cavity and the remaining cavity is controlled to realize the sorting of the target nematode, so the sorting chip can achieve high throughput and automation, and can effectively guarantee the detection efficiency.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic electrical impedance detection and sorting chip, system and method for Caenorhabditis elegans. Background technique [0002] Microfluidic technology can precisely manipulate fluids and organisms at the submicron scale, and can be applied interactively with many disciplines, such as physics, chemistry, biochemistry, biology, etc., to form a laboratory on a chip. The laboratory on a chip refers to the construction of a chemical or biological laboratory on a chip of several square centimeters, integrating basic units such as sample preparation, reaction, separation, detection, cell culture, sorting, and lysis involved in the fields of chemistry or biology. On a very small chip, a network of microfluidic channels is formed, and controllable fluids run through the network to realize various functions of conventional chemical or biological laboratories. [0003] The microstructure...

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Problems solved by technology

Generally, about 500 nematodes with the same growth cycle are needed for a biological experiment. At present, the most conventional method is to observe the length and shape of the nematodes under a stereo microscope, judge the growth stage, and use platinum probes to select th

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