Composition, preparation and application of magnetic liposome vesicles

A magnetic liposome and liposome technology, applied in transportation and packaging, chemical instruments and methods, dissolution, etc., can solve problems such as poor biocompatibility, poor emulsification performance, and instability of magnetic oleic acid vesicles , to achieve the effects of easy control, easy access to raw materials, and simple process

Active Publication Date: 2018-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The polymers in the above magnetic particle emulsifiers are all synthetic polymers, and their biocompatibility is far inferior to that of liposome materials such as ol

Method used

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  • Composition, preparation and application of magnetic liposome vesicles
  • Composition, preparation and application of magnetic liposome vesicles
  • Composition, preparation and application of magnetic liposome vesicles

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0032] Example 1: Fe 3 O 4 Nanoparticle preparation

[0033] 0.12mol FeCl 3 ·6H 2 O and 0.06mol FeSO 4 ·7H 2 O was dissolved in a four-necked flask containing 1000 mL of ultrapure water. Stir vigorously under nitrogen atmosphere, quickly add 100mL 25% concentrated ammonia water at 70℃, take out the precipitated solid particles after stirring for 20 minutes, cool to room temperature, and wash with water to neutrality to obtain Fe 3 O 4 Nanoparticles. Disperse 1 wt% of nanoparticles in 10 times volume of pH 10 buffer (borax-sodium hydroxide), drop them on a copper mesh, absorb the excess solution with filter paper, and use TEM to characterize (200kV) after natural drying. See the instructions attached figure 1 (A)Fe 3 O 4 The RT-TEM image.

Example Embodiment

[0034] Example 2: Preparation of magnetic conjugated linoleic acid vesicles CLA@Fe 3 O 4

[0035] In the 80℃ water bath, add 1.2 g of Fe 3 O 4 Add nanoparticles into a flask containing 100mL ultrapure water, ultrasonically disperse the solution, adjust the pH of the solution to pH7 with a 0.1mol / L aqueous hydrochloric acid solution, and slowly drop 10mL 17% conjugated sodium linoleate into the flask while stirring with nitrogen Aqueous solution. After 1h, remove the flask from the water bath and cool to room temperature, adjust the pH to about 5, and place a magnet on the bottom of the flask to absorb the magnetic vesicles, remove the upper liquid by magnetic decantation, and wash the magnetic vesicles with ultrapure water several times. The obtained magnetic vesicles were dried in a vacuum drying oven at 45°C to obtain magnetic conjugated linoleic acid particles (CLA@Fe 3 O 4 ).

[0036] Magnetic conjugated linoleic acid vesicle particles CLA@Fe 3 O 4 The shape and size of the pa...

Example Embodiment

[0040] Example 3: Magnetic self-crosslinking conjugated linoleic acid vesicle SCLA@Fe 3 O 4 Preparation

[0041] The 0.1g CLA@Fe obtained in Example 2 3 O 4 Vesicles were dispersed in 10mL 0.015M phosphate buffer with pH10, added to a 25mL three-necked flask, APS aqueous solution of 7% of the monomer mass was added to the flask, and reacted in a water bath at 80°C for 7 hours under stirring with nitrogen to obtain SCLA@Fe 3 O 4 Vesicle solution. Measure the absorbance A234 of the solution at 234nm before and after the self-crosslinking with an ultraviolet-visible spectrophotometer, and compare their changes to judge the self-crosslinking. See the instructions attached Figure 5 (A)CLA@Fe 3 O 4 UV spectrum before and after self-crosslinking, (B)SCLA@Fe 3 O 4 The RT-TEM image.

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Abstract

The invention discloses composition, preparation and application of magnetic liposome vesicles, and belongs to the technical field of preparation of emulsifying agents. Magnetic Fe3O4 nanoparticles induce conjugated linoleic acid (CLA) to be self-assembled into magnetic vesicles, and then, CLA molecules in bilayers of CLA@Fe3O4 are self-crosslinked to obtain stable SCLA@Fe3O4 (self-crosslinked conjugated linoleic acid) magnetic vesicles. The particle size of the prepared magnetic liposome vesicles does not exceed 25nm, the prepared magnetic liposome vesicles can be used for preparing pH/magnetic double-response Pickering emulsion, and the internal phase volume is as high as 92%. The used materials have good biocompatibility, the processes are simple, the raw materials are easy to obtain, and the sizes and the shapes of particles are easy to control.

Description

technical field [0001] The invention relates to the composition, preparation and application of magnetic liposome vesicles, in particular to the composition, preparation and application of magnetic liposome vesicles for stabilizing Pickering emulsions, and belongs to the technical field of emulsifier preparation. Background technique [0002] There are hidden dangers of accidental leakage and fire in the storage and transportation of oily liquid fuels and organic solvents. If an accident occurs, it will cause serious and irreversible damage to the ecosystem, the environment, and even human health. One of the ways to store and transport oil safely is to convert the oil into an oil-in-water emulsion, which is then broken at destination or before use. It is undoubtedly a good choice to mix oil and a small amount of water and emulsify with a particle emulsifier to prepare a stable O / W Pickering emulsion. Magnetic vesicles, that is, combinations of biocompatible magnetic nanopar...

Claims

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Application Information

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IPC IPC(8): B01F17/00C10G73/40C09K23/00
CPCC10G73/40C09K23/017
Inventor 夏咏梅魏贝方云周卓愉
Owner JIANGNAN UNIV
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