Protocatechualdehyde cross-linked collagen hydrogel and preparation method thereof

A technology of cross-linking collagen and catechin aldehyde, which is applied in the field of catechin aldehyde cross-linking collagen hydrogel and its preparation, can solve the problems of limited application and unsatisfactory effect, and achieve improved enzymolysis resistance and good biological functionality , Improve the effect of thermal stability

Active Publication Date: 2018-05-08
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemical cross-linking can improve the performance of collagen hydrogel to a certain extent, for example, using glutaraldehyde to cross-link collagen hydrogel can improve the thermal stability of collagen hydrogel, but the biotoxicity of glutaraldehyde limits its Applications in biomedicine and other fields; cross-linking with ester derivatives of carbodiimide or succinimide with less biotoxicity, although it can also improve the thermal stability and mechanical properties of collagen hydrogels, the effect is not enough ideal

Method used

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  • Protocatechualdehyde cross-linked collagen hydrogel and preparation method thereof
  • Protocatechualdehyde cross-linked collagen hydrogel and preparation method thereof
  • Protocatechualdehyde cross-linked collagen hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0034] 1) Preparation of collagen solution: Clean all the glass instruments to be used in the experiment and dry them for later use. Add 20ml of acetic acid solution with a molar concentration of 0.01mol / L and 0.1g of collagen sponge in a 50mL beaker, and place on a magnetic stirrer. Stir until the collagen sponge is completely dissolved, add 1mL of phosphate buffer solution with a molar concentration of 200mmol / L to the solution, stir evenly, and then add dropwise a sodium hydroxide solution with a molar concentration of 1mol / L under an ice bath to adjust the concentration of the solution. pH to 7.4 to obtain a collagen solution with a concentration of 5 mg / mL;

[0035] 2) Preparation of collagen fibers: Take 2 mL of the collagen solution in step 1) and transfer it to a 20×20 mm weighing bottle, and then place it in a water bath at 37°C for 2 hours to allow the collagen molecules to self-assemble into collagen after incubation fiber;

[0036] 3) Grafting reaction: take the c...

Embodiment 2～4

[0040] In Examples 2-4, according to the technical solution provided by the present invention, the ratio of raw materials and reaction conditions were adjusted within the given range, and the specific ratio of raw materials and reaction conditions are shown in Table 1.

[0041] Table 1

[0042]

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Abstract

The invention discloses a protocatechualdehyde cross-linked collagen hydrogel and a preparation method thereof. The method comprises the following steps: firstly, a collagen solution is subjected to hatching treatment to form collagen fibers by self assembly, and then the collagen fibers are soaked into a protocatechualdehyde solution for a grafting reaction; secondly, a sodium periodate solutionis added to make protocatechualdehyde in the protocatechualdehyde solution perform an oxidation self-aggregation reaction, so that intermolecular cross-linking is formed among the collagen fibers; andfinally, purification treatment is performed, and therefore the protocatechualdehyde cross-linked collagen hydrogel is obtained. According to the preparation method of the protocatechualdehyde cross-linked collagen hydrogel provided by the invention, the protocatechualdehyde is adopted as a cross-linking agent, aldehyde groups of the protocatechualdehyde react with free amino groups in collagen lysine to graft catechol groups on collagen molecules, unreacted protocatechualdehyde molecules react with the catechol groups grafted onto the collagen molecules to form cross-link bonds among the collagen molecules through oxidation self aggregation, so that the three-dimensional network structure of the collagen fibers is reinforced, and the mechanical properties and resistance to enzymatic hydrolysis of the collagen hydrogel are reinforced.

Description

technical field [0001] The invention relates to the technical field of collagen hydrogel preparation, in particular to a catechin aldehyde crosslinked collagen hydrogel and a preparation method thereof. Background technique [0002] Collagen is an extracellular matrix protein that exists in animal skin, tendons, bones, and blood vessels. Its molecular weight is about 300KDa, and it is a natural polymer material. The undenatured collagen molecule extracted from biological tissue maintains its structure in the animal body, that is, a right-handed superhelical structure formed by three polypeptide chains intertwined with each other, which has low antigenicity, good biocompatibility and biological function and many other advantages. Under physiological conditions, collagen can self-assemble to form collagen fibers, which can further form a three-dimensional network structure and bind the water filled in between, turning the solution into a hydrogel. The network structure forme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L89/00C08K5/10
CPCC08J3/075C08J3/24C08J2389/00C08K5/10
Inventor 段炼向红昭杨潇刘琳东李姣
Owner SOUTHWEST UNIV
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