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A kind of fermentation method of bacillus

A technology of Bacillus and fermentation method, which is applied in the field of Bacillus fermentation, can solve the problems that the degradation characteristics of Bacillus subtilis zearalenone cannot be satisfied, and achieve the effect of increasing unit output and reducing unit cost

Active Publication Date: 2021-03-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention patent with application publication number CN104232526A discloses a preparation process of live Bacillus subtilis preparation. The preparation process of Bacillus subtilis ANSB01G live bacteria needs to take into account both the number of live bacteria and the degradation activity of zearalenone. The fermentation method provided in CN104232526A The method cannot meet the best zearalenone degradation characteristics of Bacillus subtilis ANSB01G

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the fermentation method of Bacillus subtilis ANSB01G that the present invention relates to

[0034] The fermentation method of Bacillus subtilis ANSB01G, the steps are as follows:

[0035] (1) Primary seed preparation: 250mL triangular flask is filled with 100mL primary seed medium, sterilized by moist heat at 115°C for 20min, and inoculated into the primary seed medium with 1mL ANSB01G live bacteria freeze-dried tube strain, at a temperature of 37°C, Cultivate for 16 hours under the condition of rotating speed 180r / min.

[0036] The primary seed medium formula (g / L) is: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1g, distilled water 1000ml, pH The value is 7.2.

[0037] (2) Secondary seed preparation: 300L secondary seed medium was installed in a 500L fermenter, sterilized by moist heat at 115°C for 20 minutes, and the primary seed was inoculated in the s...

Embodiment 2

[0044] Embodiment 2: the fermentation method of Bacillus subtilis ANSB01G that the present invention relates to

[0045]The fermentation method of Bacillus subtilis ANSB01G, the steps are as follows:

[0046] (1) Primary seed preparation: 250mL triangular flask was filled with 100mL primary seed medium, sterilized by moist heat at 121°C for 15min, and 1mL ANSB01G live bacteria freeze-dried tube strains were inoculated into the primary seed medium, at a temperature of 40°C, Cultivate for 20 h at a rotational speed of 200 r / min.

[0047] The primary seed medium formula (g / L) is as follows: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1g, distilled water 1000ml, The pH value is 7.2.

[0048] (2) Secondary seed preparation: 200L secondary seed medium was installed in a 500L fermenter, sterilized by moist heat at 121°C for 15 minutes, and the primary seed was inoculated in the secon...

Embodiment 3

[0055] Embodiment 3: the preparation of Bacillus subtilis bacterial powder

[0056] The preparation method of Bacillus subtilis bacterium powder, concrete operation is: take the fermented liquid of embodiment 1 and 2 Bacillus subtilis respectively and heat to 55 ℃, enter concentrator, be concentrated under vacuum degree of 85kpa, be concentrated until solid content reaches 15 When %-30%, the material starts to be discharged; the concentrated fermented liquid is dried and granulated by multi-stage low-temperature spray fluidized drying equipment. 10 11 indivual. The inlet air temperature is 170°C, and the outlet temperature is 60-80°C.

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Abstract

The invention discloses a fermentation method of bacillus, which belongs to the technical field of microbial fermentation. The bacillus is cultured in the fermentation medium after the primary and secondary seeds are expanded. During this period, the carbon source and the restrictive amino acid that is relatively lacking in the fermentation medium are added through feed flow, and the bacillus per gram of the powder obtained by spray drying is CFU not less than 2.3×10 11 One, the spore rate is greater than 93%, which increases the number of bacillus and spore transformation rate, significantly increases the unit output, and reduces the unit cost. Bacillus subtilis ANSB01G was used for the degradation of zearalenone, and the degradation rate of zearalenone in the system could reach 92% after 48 hours.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a fermentation method of bacillus. Background technique [0002] Zearalenone (ZEN), also known as F-2 toxin, is a kind of lactone compound of 2,4-dihydroxybenzoic acid. Reproductive physiology of animals. Zearalenone is a serious hazard to animal husbandry and humans. Therefore, effective control and resolution of ZEN pollution to grain and feed is of great significance for improving animal production performance and human food safety. Scientists have conducted continuous research on the detoxification and removal methods of the toxin, and summarized the methods of ZEN detoxification and detoxification by domestic and foreign researchers, mainly including physical detoxification, chemical detoxification, and microbial and biological enzyme degradation methods. Traditional physical and chemical methods are not ideal for ZEN detoxification, which limits...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 马秋刚赵丽红计成郭永鹏郑文革
Owner CHINA AGRI UNIV