High salt-resistant phosphate solubilizing bacterial strain and application thereof

A technology of phosphate-solubilizing bacteria and salt tolerance, applied in the direction of bacteria, microbial treatment, organic fertilizers, etc., can solve the problems of poor phosphorus-dissolving performance of phosphate-solving bacteria fertilizer, low activity of phosphate-solving bacteria, and unsuitability for saline-alkali land, etc., to increase the content of available phosphorus , relieve soil compaction, improve the physical and chemical properties of the effect

Pending Publication Date: 2018-05-29
青岛滋百农生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a strain of highly salt-tolerant phosphorus-dissolving bacteria and its application, which solves the problem that microorganisms in the prior art are difficult to survive in a high-salinity environment,

Method used

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  • High salt-resistant phosphate solubilizing bacterial strain and application thereof
  • High salt-resistant phosphate solubilizing bacterial strain and application thereof
  • High salt-resistant phosphate solubilizing bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation, purification and preservation of highly salt-tolerant phosphate-solubilizing bacteria QDZN-13

[0034] The highly salt-tolerant phosphate-solubilizing bacteria QDZN-13 is isolated through domestication, cultivation, screening and purification, and the specific steps are as follows:

[0035] Acclimatization culture: Add 5g of saline-alkali soil samples from the estuary of the Yellow River in Dongying to 100mg / L inorganic phosphorus-sodium salt medium, shake and cultivate on a shaking table at 28°C and 150r / min for 7 days; then use 5% inoculation amount, In the new inorganic phosphorus-sodium salt medium, the concentration of sodium (Na) was increased in a gradient of 300 mg / L, and transferred 4 times until the sodium ion concentration reached 1200 mg / L.

[0036] Inorganic phosphorus-sodium salt medium is: per liter of inorganic phosphorus-sodium salt medium: glucose 10.0g, (NH4)2SO4 0.5g, NaCl 0.3g, KCl 0.3g, FeSO4 7H2O 0.03g, MgSO4 7H2O 0.3g, MnSO4...

Embodiment 2

[0041] Example 2 Identification of highly salt-tolerant phosphate-solubilizing bacteria QDZN-13.

[0042] Through 16SrDNA sequence analysis and physiological and biochemical experiment identification, it was determined that the strain QDZN-13 belonged to the genus Bacillus subtilis. Specific steps are as follows:

[0043]The DNA of the strain was extracted and purified using a DNA extraction kit (TransGen Biotech Co., Ltd., Beijing), and stored at 4°C. The bacterial 16SrDNA universal primers were synthesized by Huada Gene Company. The primers are as follows:

[0044] The upstream primer is 5'-AGAGTTTGATCMTGGCTCAG-3'

[0045] The downstream primer is 5'-TACGGCTACCTTGTTACGACTT-3'

[0046] Amplification reaction system: 2 μL of 10XBuffer (Mg2+), 2 μL of dNTPs, 1 μL of each primer, 1 μL of bacterial DNA, 0.5 μL of Taq DNA polymerase, and add deionized water to 20 μL.

[0047] The PCR reaction program was set as follows: pre-denaturation at 94°C for 4 min; then denaturation at ...

Embodiment 3

[0050] Example 3 Identification of highly salt-tolerant phosphate-solubilizing bacteria QDZN-13

[0051] Inoculate highly phosphate-tolerant bacterium QDZN-13 on inorganic phosphorus solid medium, culture at 28°C, and observe the characteristics of single colonies after 5 days. The characteristics of the colonies are as follows: the colonies are light milky white, transparent, with neat edges, smooth and moist, and relatively flat.

[0052] Wire-drawing experiment: Take a drop of 4% KOH solvent and drop it on a clean glass slide, and use a sterile toothpick to remove the bacteria to be tested, that is, highly salt-tolerant phosphobacteria QDZN-13. Bacteria toothpick was lifted up, no floc was observed, it can be seen that the highly salt-tolerant phosphate-solubilizing bacteria QDZN-13 is a Gram-positive bacterium.

[0053] Physiological and biochemical measurements were carried out. Through sugar fermentation experiments, it was found that the highly salt-tolerant phosphorus-...

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Abstract

The invention provides a high salt-resistant phosphate solubilizing bacterial strain and application thereof, and relates to the technical field of agricultural planting and microbes. The high salt-resistant phosphate solubilizing bacterial strain has the code name of QDZN-13, is identified to be bacillus subtilis, can survive and colonize in a high-salinity environment, has the culture collectionnumber of CGMCC No.14805, and is collected in the China General Microbiological Culture Collection Center on October 12, 2017. The high salt-resistant phosphate solubilizing bacterial strain QDZN-13can be applied to high-salinity soil, and has relatively high phosphate solubilizing activity; the high salt-resistant phosphate solubilizing bacterial strain QDZN-13 can be mixed with bacillus licheniformis and bacillus megaterium to obtain a compound microbial inoculum which is applied to a microbial fertilizer; the high salt-resistant phosphate solubilizing bacterial strain QDZN-13 can be usedas a phosphate solubilizing microbial inoculum which is applied to the microbial fertilizer alone, and is applied to a high-salinity and high-alkality land for decomposing insoluble phosphorus in soilto promote conversion of the insoluble phosphorus into soluble phosphorus capable of being absorbed and utilized by plants and promoting absorption of the plants to other nutrients. The high salt-resistant phosphate solubilizing bacterial strain has the beneficial effects of improving soil, especially the physical and chemical properties of the saline-alkali land, increasing absorbable phosphorusin the soil and effectively promoting plant growth.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a highly salt-tolerant phosphate-dissolving bacterium and its application. Background technique [0002] Phosphorus is a mineral element necessary for the normal growth of plants, and it is also a mineral element with low solubility in the soil liquid phase. Most of the phosphorus in the soil exists in the state of inorganic phosphorus or organic phosphide, which cannot be absorbed and utilized by plants. The traditional method is to add phosphorus fertilizers that plants can directly absorb and transform, such as potassium dihydrogen phosphate, monoammonium phosphate, diamine, etc., to increase the soluble phosphorus content in the soil in a short period of time, but there is sufficient phosphorus for the entire growth period of the crops. The source supply also mainly depends on the microbial activity in the soil. [0003] However, the continuous cropping of soil...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F11/08C12R1/125C12R1/10C12R1/11
CPCC05F11/00C05F11/08C12N1/20C12N1/205C12R2001/125C05F17/20
Inventor 王辉曹君正郑晗玉张伟杨振王明明姜珊王宝山
Owner 青岛滋百农生物技术有限公司
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