Composition used for skin with red blood streaks and preparation method thereof
A composition and skin technology, applied in the field of beauty and makeup, can solve the problems of inability to fundamentally remove red blood streaks, inconvenient preparation and use, and unsatisfactory cosmetic effects, so as to achieve long-term maintenance and improvement of skin red blood streaks. Effects on skin sensitivity
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Embodiment 1
[0024] A composition for red bloodshot skin, comprising the following steps:
[0025] 1. Preparation of Neural Stem Cell Lysates
[0026] The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 3 times; the transmembrane was collected by filtration with a 250nm pore size membrane, and the product was obtained. Put it in 37 ℃ and keep warm for later use.
[0027] 2. Preparation of skin stem cell culture supernatant:
[0028] Configure medium: basal medium is DMEM / F12, 25μg / LVEGF, 35μg / LEGF, 8μg / L NGF, 20mg / L human serum albumin, 25μg / L vitamin E, iron citrate 2mmol / L, glutamine 2mmol / L, gentamicin 50g / L, putrescine 1.6mg / L. Human skin stem cells were cultured in the culture medium at 37°C for 24 hours, then 1 wt% of the neural stem cell lysate prepared in step (1) was added to the culture medium, and cultured for another 24 hours, then the cell liquid was collected, centrifuged, and the supernatant w...
Embodiment 2
[0034] A composition for red bloodshot skin, comprising the following steps:
[0035] 1. Preparation of Neural Stem Cell Lysates
[0036] The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 5 times; the transmembrane was collected by filtration with a 250nm pore size membrane to obtain the final product. Put it in 37 ℃ and keep warm for later use.
[0037] 2. Preparation of skin stem cell culture supernatant:
[0038] Configuration medium: basal medium is DMEM / F12, 30μg / LVEGF, 40μg / LEGF, 10μg / L NGF, 45mg / L human serum albumin, 30μg / L vitamin E, iron citrate 3mmol / L, glutamine 2mmol / L, gentamicin 80g / L, putrescine 2.0mg / L. Human skin stem cells were cultured in this medium at 37°C for 15 hours, then 1.5wt% of the neural stem cell lysate prepared in step (1) was added to the medium, and cultured for another 60 hours, then the cell liquid was collected, centrifuged, and the supernatant was taken ,...
Embodiment 3
[0042] A composition for red bloodshot skin, comprising the following steps:
[0043] 1. Preparation of Neural Stem Cell Lysates
[0044] The human neural stem cells were cooled in liquid nitrogen for 30 minutes, thawed in a cell culture incubator at 37°C, and repeated 3 times; the transmembrane was collected by filtration with a 250nm pore size membrane, and the product was obtained. Put it in 37 ℃ and keep warm for later use.
[0045] 2. Preparation of skin stem cell culture supernatant:
[0046]Configure medium: basal medium is DMEM / F12, 25μg / LVEGF, 30μg / LEGF, 5μg / L NGF, 10mg / L human serum albumin, 20μg / L vitamin E, iron citrate 1mmol / L, glutamine 1mmol / L, gentamicin 30g / L, putrescine 1.0mg / L. Human skin stem cells were cultured in this medium at 37°C for 30 hours, then 0.6wt% of the neural stem cell lysate prepared in step (1) was added to the medium, and cultured for another 80 hours, then the cell liquid was collected, centrifuged, and the supernatant was taken , 500...
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