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Garlic pigment extract capable of preventing DNA injury

An extract and pigment technology, applied in the field of garlic pigment extract, can solve the problems that DNA protection has not been reported

Active Publication Date: 2018-06-05
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its protective effect on DNA has not been reported

Method used

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  • Garlic pigment extract capable of preventing DNA injury
  • Garlic pigment extract capable of preventing DNA injury
  • Garlic pigment extract capable of preventing DNA injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the preparation of garlic pigment extract

[0052] Store 1kg of fresh garlic at 4°C for 1 month, break the dormancy, peel and wash. Put the pulverized garlic in a beaker, add 5% acetic acid aqueous solution (v:v), and the mashed garlic turns green in 4 days at room temperature.

[0053]80% ethanol aqueous solution (v:v) with pH=3 (adjusted with acetic acid) was used for extraction at room temperature for 3 times, 12 hours each time. The material-liquid ratio of the mass of the raw material to the volume of the extract is 1:3 (g:mL). The filtrates obtained by filtering the extracted materials each time are combined and concentrated by rotary evaporation at 35°C to obtain the crude pigment extract.

[0054] The crude extract was mixed with ethyl acetate at a volume ratio of 1:1, and extracted three times to remove fat-soluble impurities such as allicin remaining in the pigment, and the ethanol extracts were combined and concentrated by rotary evaporation at...

Embodiment 2

[0058] Example 2, DNA degradation experiment induced by garlic pigment extract protecting hydroxyl radicals

[0059] Calf thymus DNA was dissolved in double distilled water to prepare a 0.15 mg / mL solution, and placed in a 4°C refrigerator overnight. Garlic pigment was prepared into 0.5, 1, 2, 10, 100, 200 and 300 mg / mL solutions with double distilled water, diluted with 30% hydrogen peroxide to a final concentration of 0.015%, and stored in a refrigerator at 4°C in the dark. Dilute 5×TBE buffer 10 times with double distilled water before use.

[0060] Preparation of 1% agarose gel: Weigh 0.4g agarose and dissolve it in 40mL electrophoresis buffer, heat it in a microwave oven or a boiling water bath until it is completely dissolved, take it out and shake it well, pour it into the agarose solution cooled to 50-60°C Add 0.4 μL of nucleic acid stain to the solution and shake well. Pour it into the glue tank, insert the sample comb, and pull out the comb after the glue is comple...

Embodiment 3

[0064] Embodiment 3, garlic pigment extract protection peroxynitrite ion (ONOO - ) induced DNA damage assay

[0065] Garlic pigment was prepared into 1, 10, 20 and 300 mg / mL solutions with double distilled water, respectively.

[0066] Preparation of peroxynitrite ion: 0.6M HCl+0.7M H at 4°C 2 o 2 with 0.6M NaNO 2 10mL of each solution flowed in through the upper port of the Y-shaped glass tube, and 20mL of 1.5M NaOH at 0°C flowed in from the bottom to quench the reaction of the first two solutions and keep the flow and velocity of the three solutions consistent. After the reaction is completed, quickly add a small amount of MnO to the reaction solution 2 Shake for 10 minutes to remove residual trace H 2 o 2 , vacuum filtration, the yellow liquid is ONOO - , placed at -20°C overnight and stored.

[0067] Preparation of 1% agarose gel: Weigh 0.4g agarose and dissolve it in 40mL electrophoresis buffer, heat it in a microwave oven or a boiling water bath until it is compl...

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PUM

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Abstract

The invention discloses a garlic pigment extract capable of preventing DNA injury. A preparation method of the garlic pigment extract comprises the following steps: crushing fresh garlic into mashed garlic, adding an acetic acid water solution into the mashed garlic until the mashed garlic becomes green; extracting the mashed garlic by virtue of an ethanol water solution I so as to obtain filtrateI, and concentrating the filtrate I, so as to obtain a pigment crude extract I; extracting the pigment crude extract I by virtue of ethyl acetate so as to obtain a filtrate II, and concentrating thefiltrate II, so as to obtain a pigment crude extract II; separating the pigment crude extract II by virtue of macroporous resin, carrying out elution by virtue of an ethanol water solution II, collecting the eluant with ultraviolet absorption effects at wavelengths of 440nm and 590nm, and carrying out concentration drying, so as to obtain a pigment crude extract III; and carrying out ultrafiltration on the pigment crude extract III by virtue of an ultrafiltration film, so as to obtain the garlic pigment extract. The preparation method of the garlic pigment extract is simple, convenient and rapid; according to the invention, the protection effect of the garlic pigment extract on DNA injury induced by hydroxyl radicals and peroxynitrite ions is disclosed for the first time, and support is provided for the application of the functional effects of the garlic pigment extract in health foods and medicines.

Description

technical field [0001] The invention relates to a garlic pigment extract capable of protecting DNA damage, and belongs to the field of extraction and processing of natural products. Background technique [0002] DNA is one of the most important substances in cell tissues, is the carrier of biological genetic information, and participates in the regulation of the expression of genetic information. And the reactive oxygen species accumulated in the oxidative stress reaction in human cells (oxygen-containing, chemically active molecules are strong oxidants. Because of the existence of unpaired electrons outside the nucleus, they have strong chemical reactivity), such as hydroxyl free Genes, etc., can attack DNA, thereby destroying the structure of DNA, causing DNA site mutations, DNA chain cleavage and other adverse reactions, leading to gene cancer and other body damage. Another peroxynitrite ion (ONOO - ) can also cause the modification and mutation of the bases of DNA, res...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54A23L33/105A61K36/8962A61P39/06A61P39/00A61K125/00
CPCA23L33/105A23V2002/00A61K36/8962A61K2236/333A61K2236/35A61K2236/39A61K2236/55C09B61/00C09B67/0096A23V2200/30A23V2250/212A23V2300/14
Inventor 王丹赵晓燕马越郭思文
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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