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Sargassum henslowianum ethyl acetate extract separated component C, preparation method thereof and application thereof to marine fouling organism control

A technology for ethyl acetate extraction and Sargassum Heinz, which is applied in the directions of botanical equipment and methods, chemicals used for biological control, applications, etc., to achieve the effects of simple separation and preparation methods, environmental friendliness, and wide sources.

Active Publication Date: 2018-06-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on whether Sargassum henzii contains active ingredients against larvae attachment and spore germination of marine fouling organisms and whether it can be applied to the field of marine fouling biocontrol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Sargassum heinzii ethyl acetate extract fraction C is extracted from Sargassum heinzii, and its separation and extraction steps include:

[0024] (1) Sargassum henslowianum C.Agardh was extracted three times with a 95% ethanol aqueous solution by volume fraction, and the extracts were combined to obtain an ethanol extract, concentrated by vacuum rotary evaporation at 45° C., and ethanol was removed to obtain a paste extract;

[0025] (2) The paste extract is resuspended with water at a ratio of 6 ml per gram, first extracted 3 times with an equal volume of petroleum ether, discarding the petroleum ether extraction components, and extracting the remaining water layer 3 times with an equal volume of ethyl acetate , the combined ethyl acetate extract was concentrated and dried to obtain the ethyl acetate extract;

[0026] (3) Carry out chromatographic separation of the ethyl acetate extract in an atmospheric silica gel column (silica gel 8000g, column length 180cm, column ...

Embodiment 2

[0029] Experimental group: Dissolve component C of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml the experimental solution. Add 1 ml of 282.6 μg / ml experimental solution into a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction C of the ethyl acetate extract of Sargassum heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then add 13ml of seawater to the Petri dish.

[0030] Control group: add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), and distribute it evenly on the bottom of the petri dish, and then add 13ml of seawater after volatilization is complete.

...

Embodiment 3

[0037] Experimental group: Dissolve component C of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml the experimental solution. Add 1 ml of 282.6 μg / ml experimental solution into a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of Fraction C of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 , and then add 13ml of seawater to the Petri dish.

[0038] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), so that the solvent is evenly distributed on the bottom of the petri dish, and after the solvent is completely evaporated,...

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PUM

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Abstract

The invention discloses a sargassum henslowianum ethyl acetate extract separated component C, a preparation method thereof and application thereof to marine fouling organism control. When the surfaceof a solid is coated with the sargassum henslowianum ethyl acetate extract separated component C at a low dose, the sargassum henslowianum ethyl acetate extract separated component C has a significanteffect of inhibiting attachment of barnacle and mussel larvae, so that the sargassum henslowianum ethyl acetate extract separated component C can be applied to preparation of a marine fouling organism control agent. The sargassum henslowianum ethyl acetate extract separated component C is extracted from sargassum henslowianum, is a natural organic compound, does not pollute a water body environment or cause enrichment thereof in organisms through food chain transport, is environment-friendly and high in safety, and does not contain heavy metals such as copper and tin while attachment of marine organisms is effectively inhibited, so that the sargassum henslowianum ethyl acetate extract separated component C has good social benefits in terms of environmental protection; in addition, the sargassum henslowianum ethyl acetate extract separated component C is wide in source and simple and convenient in separation and preparation approach, is suitable for large-scale production, and has a great popularization potential, and has a good application prospect in the marine fouling organism control.

Description

Technical field: [0001] The invention belongs to the field of natural products, and in particular relates to the separation component C of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control. Background technique: [0002] Marine fouling organisms refer to all kinds of organisms that fix or inhabit underwater parts of ships and artificial facilities and have adverse effects on human economic activities. The metal corrosion process causes corrosion and perforation; increases the dynamic load effect, causing man-made facilities to drift, unbalance or even overturn; competes with breeding objects for attachment substrates and bait, hinders their growth and development, and reduces the quality of aquatic products. Therefore, in the process of developing the marine economic industry, marine fouling biological control is always an indispensable and important part. [0003] Marine fouling organisms are composed ...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P9/00C09D5/16
CPCA01N65/00
Inventor 严涛卢虹玉曹文浩章超桦韩帅帅吴慧林明晴
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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