Serum-free culture medium of human amniotic epithelial cells

A technology of serum-free medium and epithelial cells, applied in the biological field, to achieve the effects of fast expansion, simple components, and no risk of tumorigenesis

Pending Publication Date: 2018-06-08
徐辉明
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, when hAECs are used in stem cell therapy in the future, they also face a serious problem: there are not enough good-quality hAECs for transplantation

Method used

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  • Serum-free culture medium of human amniotic epithelial cells

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1, the application of amniotic membrane epithelial cell serum-free medium in cultivating amniotic membrane epithelial cell

[0025] Preparation of serum-free medium for amniotic epithelial cells

[0026] Before cell culture, 500ml of Eplife basal culture medium (Invitrogen, M-EPI-500-CA) was added to 12.5ml of UltraGROTM-Advanced, 5ml of Supplement S7, and 5ml of L-glutamine to obtain Eplife culture system 1 for amniotic membrane epithelial cells.

[0027] In the process of cell culture, 20 mg / l of recombinant human epidermal growth factor was added to the above-mentioned amnion epithelial cell Eplife culture system 1 to obtain a serum-free medium for amnion epithelial cells.

[0028] 1. Collection of Amnion and Isolation of Amnion Epithelial Cells

[0029] The placentas of cesarean section women were collected from the East Hospital of Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine (pregnant women are required to have no obstet...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a serum-free culture medium of human amniotic epithelial cells. The invention discloses the serum-free culture medium of the human amniotic epithelial cells. The serum-free culture medium is prepared from a cell basic culture medium and culture factors, wherein by using the volume of the cell basic culture mediumas the reference, the culture factors are prepared from the following ingredients with the contents: 2 to 10 volume percent of UltraGROTM-Advanced, 1 to 5 volume percent of Supplement S7 and 1 to 5 mMof L-glutamine. The serum-free culture medium of human amniotic epithelial cells has the advantages that exogenous genes are not introduced; the cell genome stability is not changed; no animal sourceingredients are contained; no tumor causing risk exists; in addition, the stem cell transplantation safety problem due to exogenous cell mixing such as feeling layer cells is avoided; the effective in vitro cell quantity amplification can be effectively realized; the quality and the stem cell performance and epithelial performance of the amniotic endothelial cells can be maintained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a serum-free medium for human amniotic epithelial cells. Background technique [0002] Human amniotic epithelial cells (hAEC) are epithelial cells isolated from the amniotic epithelial layer of the placenta of parturients in medical waste. of adult stem cells. The amniotic membrane performs important functions in the early stages of embryonic development. It suppresses the immune response to the fetus and produces various trophic factors to support the development of the embryo. In addition, human amniotic epithelial cells provide a source of replacement cells for tissue repair in patients with tissue cell damage due to disease. At present, human amniotic epithelial cells have attracted much attention in the field of basic research, and they play a role in repairing damaged tissues in neurodegenerative diseases such as burns, diabetic foot, dry eye disease, spinal cord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0605C12N5/0625C12N2500/32C12N2501/11
Inventor 徐辉明
Owner 徐辉明
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