Biochip based on collagen membrane and preparation method thereof
A collagen film and biochip technology, applied in biochemical equipment and methods, microorganisms, general culture methods, etc., can solve the problems of poor physical and mechanical properties of collagen and the inability to form 3D patterns with strong binding force
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[0028] The preparation method of the biological chip according to the collagen film is a substrate, including steps S110 to S140.
[0029] S110, dissolve the collagen with the photosensitizer in a phosphate buffer salt solution, wherein the final concentration of collagen in the first mixture is 1 mg / ml to 10 mg / ml, and the final concentration of the photosensitizer is 0.01W. / V% ~ 0.05W / V%.
[0030] In one embodiment, collagen is a mouse tail collagen. Mouse tail collagen is readily available and has the effect of promoting adult cultured cells (especially epithelial cells). At the same time, the neutral collagen is also a natural adhesive, which is conducive to improving the adhesion of the overall biocontrol. Of course, in other embodiments, collagen can also employ other types of collagen, such as cartilage collagen, and the like.
[0031] In one embodiment, the photosensitizer is a tiger red sodium salt (RB). A certain amount of photosensitizer is added to the first mixtu...
Embodiment 1
[0076] The mouse tail collagen was dissolved from the RB photosensitizer to obtain a first mixture in a certain amount of PBS solution, wherein the final concentration of the neutral collagen in the first mixture was 5 mg / ml, the final concentration of the Rb photosensitizer was 0.04. W / V%.
[0077] The first mixture was added to the petri dish, and an ultraviolet light having a wavelength of 300 nm and an energy of 0.3 W were used to irradiate the first mixture for 10 h, so that the first mixture formed a gel shape. The air drying case was then dried in the air drying case, and a slide is then covered on the collagen film after the crosslinking reaction, and then the drying case is then placed in another 24 h to obtain a collagen film. The collateral protein film has a thickness of 150 μm ± 50 μm.
[0078] The BSA was dissolved with the RB photosensitizer to give a second mixture in a certain amount of aqueous solution, wherein the final concentration of the BSA in the second...
Embodiment 2
[0082] The rubber protein film was prepared according to the method of Example 1. The collateral protein film has a thickness of 150 μm ± 50 μm.
[0083] The fluorescein-labeled fetal bovine serum (FITC-BSA) was dissolved at a certain amount of aqueous solution to obtain a second mixture in a certain amount of an aqueous solution, wherein the final concentration of FITC-BSA in the second mixture was 180 mg / ml, Rb photosensitive agent. The final concentration was 0.2w / V%.
[0084] See image 3 The resulting collagen film is placed on a slide and placed together with collagen film in a petri dish together, placing the petri dish in an high oxygen sealing cartridge, and sets the oxygen partial pressure in high oxygen-closest positions. 0.3 kPa, the oxygen concentration was 75%, and the humidity was 60%. 1 ml of the second mixture was added to the collagen film by pipette. The 40 × oily objective lens is used, and the excitation light is emitted using a binocon excitation, the exci...
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Abstract
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