Applications of biological active peptide in skin protecting and whitening

A bioactive peptide and skin whitening technology, applied in skin care preparations, skin diseases, peptide/protein components, etc., can solve the problems of difficult penetration and limited application of glycine and cysteine, and achieve inhibition of tyrosine Enzyme activity, less side effects, good cosmetic and medicinal effects

Active Publication Date: 2018-06-26
WUHAN HONGYUE MEDICAL SCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although both glycine and cysteine ​​can significantly reduce the synthesis of melanin and play a role in whitening skin care, glycine and cysteine ​​are difficult to penetrate the cell membrane and cannot enter the dermis from the epidermis of the skin, which limits their application in the field of skin care products

Method used

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  • Applications of biological active peptide in skin protecting and whitening
  • Applications of biological active peptide in skin protecting and whitening
  • Applications of biological active peptide in skin protecting and whitening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The features and advantages of the present invention can be further understood through the following detailed description in conjunction with the accompanying drawings. The examples provided are only illustrative of the method of the present invention and do not limit the rest of the present disclosure in any way. [Example 1] This patent takes glycine-L-cysteine-L-phenylalanine tripeptide (GCF) as an example to illustrate the preparation process of the solid-phase synthetic peptide as follows:

[0037] 1. Synthesis of Complex I Fmoc-L-Phe-resin:

[0038] The tripeptide of this patent uses the method of solid-phase synthesis, weighs 10.7g of 2-chlorotrityl chloride resin (2-Chlorotrityl Chloride Resin) with a degree of substitution of 0.4mmol / g, puts the resin into a polypeptide reaction tube, and adds Dichloromethane (DCM) 160.5ml, shaken for 30 minutes; filter the DCM solvent through sand core, add 12mmol of Fmoc-L-Phe-OH amino acid, then add 40mmol of N,N-diisopropyl...

Embodiment 2

[0056] [Example 2] The bioactive peptide can enter the dermis through the epidermis

[0057] 1. Main experimental reagents and instruments

[0058] FITC, Wuhan Minghao Biotechnology Co., Ltd.; FITC-GCF and FITC-FGCGF, Wuhan Minghao Biotechnology Co., Ltd.; OCT glue, Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.; pathological tissue slicer, Germany Leitz, type 1512; Fluorescence microscope, Ningbo Sunny Instrument Co., Ltd.; paraformaldehyde, Sinopharm Chemical Reagent Co., Ltd.

[0059] 2. Experimental animals

[0060] Animal SPF grade SD male rats, weighing 240-260g. Provided by the Hubei Laboratory Animal Research Center, the animal certificate number is NO.42000600017494, and the production license number is SCXK (E) 2015-0018. Rat feed was purchased from the Experimental Animal Center of Wuhan University.

[0061] 3. Experimental principle

[0062] FITC (fluorescein isothiocyanate): It is yellow or orange-yellow crystalline powder with a molecular weight of 389.4,...

Embodiment 3

[0070] [Example 3] Bioactive peptides have a protective effect on UVB radiation human dermal fibroblasts

[0071] In this example, human dermal fibroblasts were used as the experimental object. After normal human dermal fibroblasts were treated with 1mM of each polypeptide, the cell viability was evaluated by MTT method to observe whether each polypeptide was toxic to dermal fibroblasts; UVB (radiation intensity is 1.176×10 -4 J / cm 2 ) in the case of oxidative damage, the fibroblasts were treated with 200 μM of each polypeptide, and the viability of the cells was evaluated by the MTT method, so as to determine whether each polypeptide exerted a protective effect on the oxidative damage of human dermal fibroblasts. The polypeptide used in this embodiment is as follows:

[0072]

[0073]

[0074] 1. The main reagents and instruments of the experiment

[0075] All polypeptides in the experiment were independently synthesized by our laboratory; human dermal fibroblasts we...

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Abstract

The invention provides applications of a biological active peptide with melanin synthesis inhibition activity in skin whitening, and belongs to the technical field of biological medicine and comestic.The amino acid sequence of the biological active peptide is represented by formula I: X1-(Gly-L-Gys)n-X2-X3 (I), wherein X1 is used for representing nothing or L-Phe, X2 is used for representing nothing or Gly, X3 is used for representing nothing or L-Phe, X1 and X3 are not used for representing nothing at the same time, and n is equal to or smaller than 8. The biological active peptide is capable of penetrating epidermis, entering corium layer, penetrating cell membrane, and entering cells, and is hydrolyzed into corresponding glycine and cysteine under the effect of hydrolase in cells, so that tyrosinase is inhibited, synthesis of melanin is inhibited, and effective skin whitening is achieved. The biological active peptide can be used for preparing a plurality of preparations used for preventing and treating human body chromatosis diseases and melanoma caused by excess melanin, and other preparations used for inhibiting the activity of tyrosinase.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and cosmetics, and relates to the application of a class of bioactive peptides in skin care and skin whitening. Background technique [0002] Heart of beauty in everyone. For women, whitening is an eternal topic, and cosmetics with whitening effects have always been favored by oriental women. In recent years, in order to meet the needs of women for whitening, a series of whitening and whitening cosmetics such as concealer, foundation, and makeup powder have appeared on the market. They have instant whitening effects, but they have no effect on fundamentally improving skin quality. In fact, only by regulating the internal skin texture and eradicating melanin, the culprit that affects whitening, can the skin show a truly bright and white radiance. [0003] Melanin is actually an amino acid derivative, which is present in everyone's body. Melanin is formed in the melanocytes at the base of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61Q19/02A61K38/06A61K38/08A61K38/10A61K38/07A61P17/00
CPCA61K8/64A61K38/06A61K38/07A61K38/08A61K38/10A61K2800/782A61Q19/02
Inventor 万芪陈娟庄杨张娅廖华宝万沙
Owner WUHAN HONGYUE MEDICAL SCI INC
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