Nanometer diagnosis and treatment agent, preparation method and application thereof
A nanometer and nanoparticle technology, which is applied in the field of nanodiagnostic agents and nanomaterials for tumor treatment, can solve the problems of underutilization, insufficient curative effect, and increased concentration, and achieve good application prospects, easy industrial production, and convenient operation. Effect
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Embodiment 1
[0049] Example 1 Synthesis of hollow mesoporous organosilicon nanoparticles (HMON)
[0050] First, 74 mL of ethanol, 10 mL of ultrapure water and 3.14 mL of ammonia solution were mixed in a water bath at 30°C and stirred for 5 minutes, then 6 mL of tetraethyl orthosilicate (TEOS) was added rapidly and stirred for 1 hour to obtain solid SiO 2 nanoparticles. 2 g CTAC and 0.04 g triethanolamine (TEA) were added to 90 mL of ultrapure water and stirred for 1.5 h, and 30 mL of the solid SiO prepared above was added 2 The nanoparticle solution was stirred for 1.5 hours. 1.8 mL of BTES was added dropwise to the above solution and stirred at 80 °C for 1 hour to obtain a solid SiO coated with a layer of mesoporous silicone on the surface. 2 nanoparticles. Finally, the prepared surface is wrapped with a layer of mesoporous silicone solid SiO 2 Nanoparticles dispersed in 0.6 mol / L Na 2 CO 3 The solution was stirred at 80° C. for 1 hour, centrifuged, and washed with ultrapure water t...
Embodiment 2
[0054] Example 2 Synthesis of L-Arg-HMON-GOx
[0055] Aminated GOx was obtained by dissolving 38 mg EDC, 57 mg NHS and a certain amount of GOx in 6 mL ultrapure water, adding 45 μL APTES, and stirring at room temperature for 8 hours. Then, 20 mg of HMON was dispersed in 4 mL of ultrapure water, and the above solution was added to continue stirring for 24 hours to obtain GOx-grafted HMON (HMON-GOx) particles. Finally, 20 mg of HMON-GOx and 200 mg of L-Arg were dispersed in 10 mL of ultrapure water and stirred (stirring) at room temperature for 24 hours to obtain a multifunctional HMON (L-Arg-HMON-GOx) co-transporting GOx and L-Arg (L-Arg-HMON-GOx). Nanomedicine. The roadmap and TEM image of the synthesized HMON simultaneously loaded (co-transported) with GOx and L-Arg are shown in figure 2 shown.
[0056] figure 2 In (a) shows the route for the synthesis of HMON with simultaneous loading (co-transport) of GOx and L-Arg, where APTES stands for 3-aminopropyltriethoxysilane,...
Embodiment 3
[0059] The standard MTT method was used to evaluate the effect of starvation / gas therapy synergistic therapy on the survival rate of U87MG cells. U87MG cells at 1 × 10 per well 4 Density seeded into 96-well plates and placed at 37°C, 5% CO 2 Incubate for 24h under conditions. Next, the old medium in the 96-well plate was aspirated, and sugar-free DMEM medium (no glucose) and sugar (100 μg / mL HMON-GOx, HMON-L-Arg and L-Arg-HMON-GOx) containing 200 μg / mL HMON-GOx, HMON-L-Arg and L-Arg-HMON-GOx were added. / mL glucose) DMEM medium. After culturing for 24 hours, the old medium in the 96-well plate was aspirated, and 100 μL of MTT medium solution (0.8 mg / mL) was added to each well, and the culture was continued for 4 hours. Add 100 μL of DMSO solution to the well, and after shaking gently, detect the OD value of each well on a Bio-TelEL×800 microplate reader (detection wavelength is 570 nm), and use the following formula to calculate the cell viability. )(%)=(OD570 value of sam...
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