Method for measuring chitosan content with resonance Rayleigh scattering method by carmine red as probe

A resonant Rayleigh scattering and chitosan technology, which is applied in the measurement of scattering properties and the preparation of samples for testing, can solve the problems of non-specificity of samples, low sensitivity and high cost, and achieve good reproducibility and high sensitivity. , the effect of easy operation

Inactive Publication Date: 2018-06-29
GUANGDONG PHARMA UNIV
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Problems solved by technology

The spectrophotometric method reported above has the disadvantages of low sensitivity and non-specificity when measuring samples, while liquid

Method used

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  • Method for measuring chitosan content with resonance Rayleigh scattering method by carmine red as probe
  • Method for measuring chitosan content with resonance Rayleigh scattering method by carmine red as probe
  • Method for measuring chitosan content with resonance Rayleigh scattering method by carmine red as probe

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experiment example

[0027] 1. Resonance Rayleigh scattering spectrogram

[0028] In a 10mL colorimetric tube, add an appropriate amount of chitosan solution with a concentration of 10μg / mL, 1.5mL of glycine-hydrochloric acid buffer solution with pH=3.0, and 1.0mL of carmine solution with a concentration of 1.0×10-4mol / L, Dilute the volume to the mark with distilled water, shake well, configure the reaction system with chitosan concentration of 0.50, 1.00, 1.50, 2.0, 2.50 μg / mL, heat in a 60°C water bath for 10min, and cool to room temperature. Set blank group and standard group. Synchronously scan at λex=λem on the fluorescence spectrophotometer to obtain the resonant Rayleigh scattering spectrum. Depend on figure 1 The results shown show that the concentration of 1-6 chitosan increases successively, the scattering value of the blank group in the experiment is low, and the scattering value increases significantly after the combination of CTS and carmine, and with the increase of the concentrati...

Embodiment

[0046] A kind of method that utilizes carmine as the resonance Rayleigh scattering method of probe to measure chitosan content, it comprises the steps:

[0047] Drawing of standard curve: In a series of 10mL colorimetric tubes, add chitosan CTS standard solution (10μg / mL) (0.5, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50mL) in sequence, and at the same time add reagent Blank as a reference, pH 3.0 glycine-hydrochloric acid buffer solution 1.0mL, carmine P4R (1.0×10-4mol / L) 1.0mL, diluted with distilled water to the mark, mixed evenly to obtain a concentration gradient of (0.5, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50μg / mL) standard solution. Heat in a water bath at 60°C for 10 min, and cool to room temperature. , detect the resonance Rayleigh scattering intensity of the chitosan solution to be tested, specifically carry out synchronous scanning with λex=λem, record the RRS spectrum, and measure the I of the ion association and the I of the reagent blank at 366nm respectively, survey I, Cal...

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Abstract

The invention discloses a method for measuring chitosan content with a resonance Rayleigh scattering method by carmine red as a probe. Research shows that chitosan can be combined with carmine red toform an ion-association complex, resonance scattering strength is remarkably enhanced, chitosan concentration has a good linear relation with delta I in a certain concentration range, and by means ofthe linear relation as quantification basis of chitosan, the method for measuring chitosan content with the resonance Rayleigh scattering method by carmine red as the probe is established. The methodhas the advantages of being simple to operate, fast, low in cost, high in sensitivity, high in recovery rate, good in repeatability and the like and is suitable for being popularized and used in actual application.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to the technical field of chitosan content determination, and more specifically to a method for measuring chitosan content by a resonance Rayleigh scattering method using carmine as a probe. Background technique [0002] Chitosan, whose chemical name is polyglucosamine (1,4)-2-amino-2-deoxy-D-glucan, is a kind of insect shell, shrimp shell, crab shell, mollusk widely present in nature Natural macromolecular substances in the bones and bones of certain algae. With continuous research, it has been found that chitosan has biological functions such as anti-tumor, anti-coagulation, anti-bacterial, weight loss and lipid-lowering and immunity-enhancing functions, making it widely used in medicine, food, chemical industry, cosmetics, water treatment, Applications in many fields such as metal extraction and recovery, biochemical and biomedical engineering have been greatly deve...

Claims

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Application Information

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IPC IPC(8): G01N1/38G01N21/51
CPCG01N1/38G01N21/51
Inventor 白研苏政权毋福海
Owner GUANGDONG PHARMA UNIV
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