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A high-capacity macroporous polymer cation exchange chromatography medium and its preparation

A technology for cation exchange and chromatography medium, which is applied in the field of high-capacity, large-pore size polymer cation exchange chromatography medium and its preparation, can solve the problems of increased preparation cost, many reaction steps, waste, etc., and achieves high mechanical strength and fluidity. Mass transfer performance, fast operating flow rate, and small reaction steric hindrance

Active Publication Date: 2020-01-17
BEIJING INSTITUTE OF PETROCHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many reaction steps, and because it is difficult to quantitatively control the reaction of each step, it is necessary to add an excessive amount of reaction reagents, resulting in a certain amount of waste and increasing the preparation cost.

Method used

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  • A high-capacity macroporous polymer cation exchange chromatography medium and its preparation
  • A high-capacity macroporous polymer cation exchange chromatography medium and its preparation
  • A high-capacity macroporous polymer cation exchange chromatography medium and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: High-capacity large-pore diameter PGMA-EDMA cation exchange chromatography medium

[0033] Schematic diagram of the preparation process of cation exchange chromatography medium by graft copolymerization on the surface of macroporous polymer microspheres. figure 1 .

[0034] The preparation method of high-capacity large-pore diameter PGMA-EDMA cation exchange chromatography medium comprises the following steps:

[0035] (1) Swelling treatment of macroporous polyglycidyl methacrylate-ethylene glycol dimethacrylate (PGMA-EDMA) microspheres (average pore size 128nm) in acrylic acid solution

[0036] Accurately weigh 1.0 g of PGMA-EDMA microspheres and put them into a 50 mL three-necked reaction flask, then add 3.0 mL of acrylic acid dimethyl sulfoxide solution, swell for 2.0 h under mechanical stirring at 100 rpm, and fill high-purity nitrogen for protection during the swelling process .

[0037] (2) Acrylic acid grafted on the surface of PGMA-EDMA microsph...

Embodiment 2

[0048] Embodiment 2: High-capacity large-pore PEDMA cation-exchange chromatography medium

[0049] The preparation method of high-capacity large-pore PEDMA cation exchange chromatography medium comprises the following steps:

[0050] (1) Swelling treatment of macroporous PEDMA microspheres (average pore diameter 687nm) in 2-acrylamido-2-methyl-1-propanesulfonic acid solution

[0051] Accurately weigh 1.0g of PEDMA microspheres into a 50mL three-neck reaction flask, then add 5.0mL of dimethylformamide solution of 2-acrylamido-2-methyl-1-propanesulfonic acid, and swell under mechanical stirring at 100rpm 1.0h, filled with high-purity nitrogen for protection during swelling treatment.

[0052] (2) Graft polymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid on the surface of PEDMA microspheres

[0053] Weigh 0.049g CuCl (1 / 20 mass of microspheres) and 0.147g tetramethylethylenediamine (TMEDA) (3 times the mass of CuCl) and add them to the microspheres after swelling tre...

Embodiment 3

[0055] Embodiment 3: High capacity large pore size PSt-EDMA cation exchange chromatography medium

[0056] The preparation method of PSt-EDMA cation exchange chromatography medium with high loading capacity and large pore size comprises the following steps:

[0057] (1) Macroporous PSt-EDMA microspheres (average pore size 2100nm) are pretreated in methacrylic acid solution

[0058] Accurately weigh 1.0 g of PSt-EDMA microspheres into a 50 mL three-necked reaction flask, then add 10 mL of methacrylic acid in dioxane solution, swell for 0.5 h under mechanical stirring at 100 rpm, and fill high-purity nitrogen gas for protection during the swelling process .

[0059] (2) Grafting and polymerizing methacrylic acid on the surface of macroporous PSt-EDMA microspheres

[0060] Weigh 0.016g CuCl (1 / 60 mass of microspheres) and 0.048g N,N,N,'N,"N,"'-pentamethyldiethylenetriamine (PMDETA) (3 times the mass of CuCl) and add Into the microspheres after the swelling treatment in step (1...

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Abstract

The invention discloses a high-carrying-capacity and large-pore-diameter polymer cation exchange chromatography medium and preparation thereof and belongs to the technical field of preparation and application of polymer chromatography media. The cation exchange chromatography medium takes a polyacrylate or polystyrene microsphere as a base material and the surface of the microsphere is grafted with a vinyl functional monomer. A preparation method of the high-carrying-capacity and large-pore-diameter polymer cation exchange chromatography medium comprises the following steps: carrying out swelling treatment on the polyacrylate or polystyrene microsphere in a vinyl functional monomer solution; adding an atom transfer free radical polymerization catalyst ligand system into the microsphere subjected to the swelling treatment and carrying out grafting polymerization reaction; after the polymerization reaction is finished, removing an unreacted monomer and drying in vacuum to obtain the cation exchange chromatography medium. The prepared cation exchange chromatography medium has the advantages of high ion exchange capacity, high protein binding carrying capacity and rapid operation flow,and is suitable for rapidly separating and purifying biological macromolecules.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of polymer chromatographic media, and in particular relates to a polymer cation exchange chromatographic media with high load and large pore size and its preparation. Background technique [0002] With the rapid development of biopharmaceutical technology, the scale of biopharmaceutical production is expanding day by day, which poses new challenges to its downstream separation and purification. It is imperative to develop fast and efficient purification methods. Among them, the separation medium is the core of the separation and purification technology. At present, the commonly used separation medium is mostly based on polysaccharides. This type of medium has the characteristics of soft texture (withstand pressure less than 0.3MPa) and small pore size (30-50nm). The amount of separation and purification applications is subject to certain restrictions. In order to achieve high-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F265/04C08F220/06C08F267/06C08F220/58C08F257/02B01J39/20
CPCB01J39/20C08F257/02C08F265/04C08F267/06C08F2438/01
Inventor 张荣月公丕胜靳海波何广湘
Owner BEIJING INSTITUTE OF PETROCHEMICAL TECHNOLOGY
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