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Double-inoculation method for improving plant resistance in heavy metal contaminated soil

A technology of polluted soil and plant resistance, applied in the restoration of polluted soil, microorganism-based methods, biochemical equipment and methods, etc. To achieve the effect of promoting accumulation, improving resistance and phytoremediation efficiency, and increasing biomass

Inactive Publication Date: 2018-07-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of non-hyperaccumulative plant alfalfa with small biomass in the extraction of heavy metals, the growth is easily poisoned by heavy metals, and the lack of soil nutrients in heavy metal-polluted areas, and the proposed new double inoculation method can improve the phytoremediation efficiency of heavy metal-contaminated soil

Method used

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  • Double-inoculation method for improving plant resistance in heavy metal contaminated soil
  • Double-inoculation method for improving plant resistance in heavy metal contaminated soil
  • Double-inoculation method for improving plant resistance in heavy metal contaminated soil

Examples

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Embodiment example 1

[0039] A double inoculation method for improving plant resistance in heavy metal polluted soil, comprising the following steps:

[0040] Step 1: Cultivation of strains and acquisition of bacterial suspension:

[0041] The strains used in the double inoculation system are rhizobia (S.meliloti CCNWSX0020) and growth-promoting bacteria jelly-like Bacillus (P.Mucilaginosus ACCC10013), among which the rhizobia S.meliloti CCNWSX0020 was grown by our research group from the copper tailings area in Fengxian County, Shaanxi Province. The root nodules of Tianlan alfalfa were isolated and preserved in the China Agricultural Microorganism Collection Center with the preservation number ACCC19736; the growth-promoting bacterium P. Mucilaginosus was provided by the China Agricultural Microbiology Collection Center with the preservation number ACCC10013.

[0042] The medium used in the strain cultivation process is as follows:

[0043] Mannitol yeast solid medium (YMA): mannitol (10g / L), yea...

Embodiment example 2

[0064] A double inoculation method for improving plant resistance in heavy metal polluted soil, comprising the following steps:

[0065] Step 1: Cultivation of strains and acquisition of bacterial suspension:

[0066] The strains used in the double inoculation system are rhizobia (S.meliloti CCNWSX0020) and growth-promoting bacteria jelly-like Bacillus (P.Mucilaginosus ACCC10013), among which the rhizobia S.meliloti CCNWSX0020 was grown by our research group from the copper tailings area in Fengxian County, Shaanxi Province. The root nodules of Tianlan alfalfa were isolated and preserved in the China Agricultural Microorganism Collection Center with the preservation number ACCC19736; the growth-promoting bacterium P. Mucilaginosus was provided by the China Agricultural Microbiology Collection Center with the preservation number ACCC10013.

[0067] The medium used in the strain cultivation process is as follows:

[0068] Mannitol yeast solid medium (YMA): mannitol (10g / L), yea...

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Abstract

The invention discloses a double-inoculation method for improving plant resistance in heavy metal-contaminated soil. The double-inoculation method comprises the following steps: step 1, culture of a bacterial strain and acquisition of a bacterial suspension, wherein the bacterial strain used in a double-inoculation system is wild rhizobacteria (S.meliloti CCNWSX0020) and plant growth-promoting rhizobacteria gelatinous bacillus (P.Mucilaginosus ACCC10013); step 2, performing germination treatment on alfalfa seeds; step 3, performing double inoculation on rhizobacteria-bacillus on alfalfa. The double-inoculation method disclosed by the invention has the benefits that the toxic effects of heavy metals on plants are alleviated by constructing the double-inoculation method for rhizobacteria andplant growth-promoting rhizobacteria, the plant biomass is increased, the plant resistance and the ability to extract the heavy metals are improved, meanwhile the soil nutrient circulation in a contaminated area is promoted, and a new strategy is provided for bioremediation technology plant management measures for heavy metal-contaminated soil in a mining area.

Description

technical field [0001] The invention belongs to the technical field of heavy metal polluted soil control and restoration, and relates to a double inoculation method for improving plant resistance and restoration efficiency in heavy metal polluted soil. Background technique [0002] The mining and smelting of mineral resources, the excessive application of pesticides and fertilizers, etc., cause heavy metals to accumulate in the soil and are not easily degraded by microorganisms, which has caused great harm to the ecosystem and human health for a long time. Phytoremediation of heavy metal-contaminated soil is an environmentally friendly and low-cost remediation technology, which has attracted widespread attention from the society because it is easy to implement and green and healthy. The plant-microbe joint remediation is a new technology based on phytoremediation, forming a mutually beneficial association with microorganisms to enhance plant resistance and increase plant bio...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/00B09C1/10C12R1/41C12R1/07
CPCB09C1/10B09C1/105B09C2101/00C12N1/20
Inventor 方临川鞠文亮王云强刘磊韦革宏
Owner NORTHWEST A & F UNIV
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