Subculture medium for Pleione bulbocodioides and preparation method thereof

A subculture medium, the technology of Suanlanlan, which is applied in botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of low pollen pollination rate, incomplete development of seed embryos, and small number of pods grown and other problems, to achieve the effect of factory production, increase growth rate, and overcome hypoplasia

Inactive Publication Date: 2018-08-03
康美药业(昆明)种质资源有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] At present, most of the solitary orchids circulating in the market are wild, and a few are artificially cultivated; most of the solitary orchids are propagated by divisions. In the natural environment, the pollen pollination rate is low, and the number of pods grown is also small, and the seed embryos are well developed. Incomplete, can only be seeded in culture medium under sterile conditions for tissue culture

Method used

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  • Subculture medium for Pleione bulbocodioides and preparation method thereof
  • Subculture medium for Pleione bulbocodioides and preparation method thereof
  • Subculture medium for Pleione bulbocodioides and preparation method thereof

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preparation example Construction

[0026] The preparation method of this Suanlan subculture medium comprises,

[0027] Solution pre-preparation step: prepare macroelement solution, trace element solution, iron salt solution, organic component solution and potato juice respectively;

[0028] Mixing steps: mix the macroelement solution, trace element solution, iron salt solution, organic component solution, potato juice, indole acetic acid, 6-benzyl adenine, activated carbon powder, sucrose and agar powder, adjust the pH, and then Containing, sterilizing, cooling, and solidifying, the Suanlan subculture medium is obtained. Wherein, the preparation process of the potato juice is as follows: take 30-50g of potatoes and pour them into 1L of sterile water to pulverize to form the potato juice containing potato powder.

[0029]As a further embodiment, in the solution pre-allocation step, the preparation method of the macroelement solution is as follows: potassium nitrate, ammonium nitrate, calcium chloride dihydrate,...

Embodiment 1-3

[0035] Example 1-3 Suanlan subculture medium

[0036] The raw materials were weighed according to the proportions in Table 1, and prepared according to the following steps. The difference is that the added raw material proportions are different, and the Suanlan subculture product is prepared. See Table 1 for details:

[0037] Table 1: Raw material ratio table of embodiment 1-3

[0038]

[0039]

[0040] The preparation method of the above-mentioned Suanlan subculture medium includes,

[0041] Solution pre-preparation step: prepare macroelement solution, trace element solution, iron salt solution, organic component solution and potato juice respectively. Specifically as follows: the preparation method of the described macroelement solution is as follows: potassium nitrate, ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate, and potassium dihydrogen phosphate are dissolved respectively in the formula quantity, and then potassium nitrate, ammoniu...

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Abstract

The invention discloses a subculture medium for Pleione bulbocodioides and a preparation method thereof. The subculture medium is a 1 / 2 MS medium, and the 1 / 2 MS medium contains cane sugar with a concentration of 2.0 g / L, agar powder with a concentration of 4.8 g / L, indoleacetic acid with a concentration of 0.2 mg / L, 6-benzyladenine with a concentration of 1.8 mg / L, potato juice with a concentration of 30-50 g / L and active carbon powder with a concentration of 0.3 g / L. When used for tissue culture, the subculture medium provided by the invention has the advantages that the subculture medium overcomes the problems of incomplete development of seed embryos, low reproduction rates in natural environments and the like of seeds; and the subculture medium can effectively improve the breeding speed breeding quantity of the seedlings of Pleione bulbocodioides, controls germplasm stability from the source of female parents, guarantees the quality of the seedlings, realizes industrial productionof high-quality seeds and seedlings of Pleione bulbocodioides, meets ornamental and medicinal demands of Pleione bulbocodioides, and reduces excess digging of wild Pleione bulbocodioides.

Description

technical field [0001] The invention relates to a technique for raising seedlings by tissue culture, in particular to a subculture culture medium for orchid albicans and a preparation method thereof. Background technique [0002] Pleione bulbocodioides (Franch.) Rolfe, Orchidaceae only Pleione bulbocodioides, ground or epiphytic herb. It grows in forests at an altitude of 1100-3500 meters or on rocky ground or moss-covered rocks at the edge of forests, and is also common on grassy slopes with slightly shaded gravel. Mainly distributed in the southwest of Sichuan, China (Yuexi, Yanyuan, Shimian, Xichang, Muli), the west to the north of Guizhou (Kaili, Yinjiang, Leishan, Guiyang, Fanjingshan, Panxian, Anlong, Wangmo, Nayong), northwestern to southeastern Yunnan (Gongshan, Weixi, Lijiang, Dali, Yangbi, Yongning, Mengzi, Wenshan), southeastern Tibet (Chawarong), and northern Myanmar. The rhizome is used as medicine, which has the effects of clearing heat and detoxification, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 许冬瑾严新
Owner 康美药业(昆明)种质资源有限公司
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