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Preparation method of gene-loading porous magnetic microspheres

A technology of magnetic microspheres and genes, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of large side effects and low efficiency, achieve good spheroidization, reduce The effect of cost and easy operation

Inactive Publication Date: 2018-08-10
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main problem facing the current lung cancer treatment is that the efficiency of drug delivery to the lungs through conventional systemic drug delivery is very low, and it will also bring great side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Heating in a water bath at 60°C and N 2 Under protection, 600 mg Fe 3 o 4 Place in a three-neck flask filled with 80 mL of 50 wt% ethanol, stir mechanically for 30 min, add 1 mL of 3-aminopropyltriethoxysilane, adjust the pH to 4.0 with glacial acetic acid, and react with mechanical stirring for 12 h; The finished product is separated by magnetic field, washed with absolute ethanol and absolute ether in sequence until there is no oily suspension, placed in a drying oven, and vacuum-dried at 45°C to obtain the amino-protonated Fe 3 o 4 Nanoparticles;

[0025] (2) Take 10 mg of the amino-protonated Fe prepared in step (1) 3 o 4 Nanoparticles were dispersed in a beaker filled with deionized water, and the aqueous solution containing 50 μg siRNA was added dropwise under mechanical stirring, continued to stir for 6 h, washed with deionized water, and magnetically separated / redissolved several times to obtain Fe 3 o 4 / siRNA composite particles;

[0026] (3) Disso...

Embodiment 2

[0032] (1) Heating in a water bath at 60°C and N 2 Under protection, 1000 mg Fe 3 o 4 Placed in a three-necked flask filled with 150 mL of 50 wt% ethanol, stirred mechanically for 30 min, adjusted the pH to 4.0 with glacial acetic acid, added 4 mL of 3-aminopropyltriethoxysilane, and stirred mechanically for 12 h; The finished product is separated by magnetic field, washed with absolute ethanol and absolute ether in sequence until there is no oily suspension, placed in a drying oven, and vacuum-dried at 45°C to obtain the amino-protonated Fe 3 o 4 Nanoparticles;

[0033] (2) Take 50 mg of amino-protonated Fe prepared in step (1) 3 o 4 Nanoparticles were dispersed in a beaker filled with deionized water, and the aqueous solution containing 100 μg siRNA was added dropwise under mechanical stirring, continued to stir for 6 h, washed with deionized water, and magnetically separated / redissolved several times to obtain Fe 3 o 4 / siRNA composite particles;

[0034] (3) Dissol...

Embodiment 3

[0040] (1) Heating in a water bath at 60°C and N 2 Under protection, 800 mg Fe 3 o 4 Place in a three-neck flask filled with 100 mL of 50 wt% ethanol, stir mechanically for 30 min, add 1.5 mL of 3-aminopropyltriethoxysilane, adjust the pH to 4.0 with glacial acetic acid, and react with mechanical stirring for 12 h; The finished product is separated by magnetic field, washed with absolute ethanol and absolute ether in sequence until there is no oily suspension, placed in a drying oven, and vacuum-dried at 45°C to obtain the amino-protonated Fe 3 o 4 Nanoparticles;

[0041] (2) Take 20 mg of the amino-protonated Fe prepared in step (1) 3 o 4 Nanoparticles were dispersed in a beaker filled with deionized water, and the aqueous solution containing 80 μg siRNA was added dropwise under mechanical stirring, and the stirring was continued for 6 h, washed with deionized water, and magnetically separated / redissolved several times to obtain Fe 3 o 4 / siRNA composite particles;

...

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Abstract

The invention discloses a preparation method of gene-loading porous magnetic microspheres and belongs to the field of preparation of biological materials. An emulsification-solvent evaporation technology is adopted and PLGA is used as a sphere forming material, Span-80 is used as an emulsifier and Fe3O4 / siRNA is used as a template drug to prepare the gene-loading porous magnetic microspheres. According to the preparation method, the PLGA is used as a sphere forming substrate and has the characteristics of good sphere forming performance, good biocompatibility and capability of being degraded in bodies; meanwhile, an amino-protonated ferroferric oxide electrostatic gene is used and has a relatively high specific surface area so that the nucleic acid loading amount is relatively high and double synergistic effects of magnetic hyperthermia and gene therapy can be realized. Furthermore, the method disclosed by the invention has the advantages that the cost can be reduced and the operationis simple and convenient; the prepared porous magnetic microspheres with the synergistic effects are used for treating lung cancer through suction type drug administration.

Description

technical field [0001] The invention belongs to the field of biological material preparation, and in particular relates to a method for preparing gene-loaded porous magnetic microspheres. Background technique [0002] In the treatment of tumors, although drug therapy has made great progress, the inherent acquired drug resistance still needs to be overcome, otherwise it is difficult to achieve successful treatment and eventually lead to the death of patients. In addition, drug resistance usually occurs during chemotherapy engineering or post-relapse chemotherapy. Tumor cells produce antibodies to different structures and types of drugs, resulting in a high recurrence rate and making the tumor more difficult to cure. Many factors are involved in the occurrence of intrinsic or acquired multidrug resistance, which hinders drug delivery to targeted tumor tissues and reduces its killing effect on tumor cells, making tumor cells less sensitive to drugs. However, these drug resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/72A61K9/16A61K47/34A61K47/02A61K31/7088A61K41/00A61P35/00
CPCA61K9/007A61K9/1641A61K31/7088A61K41/0052A61K47/02A61K47/34A61P35/00A61K2300/00
Inventor 游力军刘小翠方哲翔张其清
Owner FUZHOU UNIV
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