Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for extracting sophoricoside from fructus sophorae and anti-inflammation application of sophoricoside

An extraction process and technology of sophora japonica, which are applied in the preparation of anti-inflammatory agents, non-central analgesics, sugar derivatives, etc., can solve the problems of large enzyme dosage and low extraction rate, and achieve the improvement of extraction rate and purity, and dissolution. High rate and high efficiency

Active Publication Date: 2018-08-10
孙波
View PDF8 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many techniques for extracting Sophora flavonoids, most of which mainly use chemical reagents for extraction, while conventional extraction methods have unsafe factors such as organic solvent residues, which pose a potential threat to human health. Improvements have been made, such as "Process optimization of enzymatic extraction of total flavonoids in Sophora japonicus, Food Technology Science 2012" used enzymatic method to extract flavonoids from Sophora japonicus, which has the advantages of non-toxicity and mild reaction conditions, but this method has the advantages of enzyme dosage Larger, lower extraction rate and other defects, it needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for extracting sophoricoside from fructus sophorae and anti-inflammation application of sophoricoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The technology of extracting sophorin from Sophora japonica, it comprises the following steps:

[0028] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying→dissolving→crystallization.

[0029] Specifically include:

[0030] Take Sophora japonicus, pulverize, and pass through a 100-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 3MPa, hold the pressure for 30s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 5 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 20,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction...

Embodiment 2

[0032] The technology of extracting sophorin from Sophora japonica, it comprises the following steps:

[0033] Crushing→high pressure→electric field treatment→shearing→enzyme reaction→microwave alcohol extraction→evaporation→drying→adsorption→elution→evaporation→drying→dissolving→crystallization.

[0034] Specifically include:

[0035] Take Sophora japonicus, pulverize, pass through a 200-mesh sieve to obtain Sophora japonicus powder, then place it in a reactor, control the pressure in the reactor to 2MPa, hold the pressure for 40s, and perform pulse electric field treatment. The field strength of the electric field is 10kv / cm, and the total treatment The time is 200 μs, the pulse width is 4 μs, and the pulse frequency is 400pps; then add 4 times the weight of water, use a high-speed shearing machine to cut for 3 minutes, and the shear speed is 5000rpm, and then add according to the ratio of 10,000 U:100g Sophora japonicus powder For cellulase, control the enzyme reaction tem...

Embodiment 3

[0049] Determination of total flavonoids: Taking Huaijiao in southern Shandong as an example, the experiment of the present invention uses 1000g Huaijiao, and the content of total flavonoids in Huaijiao powder measured by HPLC method is 19.7%, and the experimental groups are respectively selected from Example 1 and Comparative Example 1- 4. Obtain the weight of total flavonoids extract by weighing, measure the total flavonoids content in the extract by HPLC method, calculate the yield of total flavones, calculation formula: yield=((quality × content of extract) / (sophora japonicus quality × Content)) × 100%. The specific results are shown in Table 1:

[0050] Table 1

[0051] group

[0052] The present invention also detects the weight of the crystals of Example 1 and Comparative Examples 1-4 and the content of sophorin in the crystals, see Table 2 for details:

[0053] Table 2

[0054] group

[0055] As shown in the above table 1-2, the yield and purity...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant extraction, and discloses a process for extracting sophoricoside from fructus sophorae. The process includes steps of smashing, high-pressure treatment, electric field treatment, shearing, enzymatic reaction, microwave alcohol extraction, evaporation, drying, adsorption, elution, evaporation, drying, dissolution and crystallization. The processhas the advantages of simplicity, feasibility and high total flavone and sophoricoside extraction rates and purity.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a process for extracting sophorin from Sophora japonica and its application in anti-inflammation. Background technique [0002] Sophora japonica L. is the fruit of Sophora japonica L., a leguminous plant. It is a commonly used traditional Chinese medicinal material recorded in the Pharmacopoeia. The medicinal Sophora japonica L. should usually be harvested in winter. Ripe pods. The dried Sophora japonicus pods picked after the winter solstice are cylindrical, sometimes curved, and the seeds are constricted into beads. After the foreskin, they are brown or yellowish brown, and the seeds are brownish black; Chinese medicine theory believes that Sophora japonicus is cold in nature and has a strong taste. Bitter, returns to the liver and large intestine meridian, has the functions of clearing away heat and purging fire, cooling blood and stopping bleeding. It is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H15/26C07H1/08A61P29/00
CPCA61P29/00C07H1/08C07H15/26
Inventor 孙波王芳王洲
Owner 孙波
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com