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Preparation method and application of porous silicon material for biological sample pretreatment

A biological sample and porous silicon technology, which is applied in the preparation of test samples, analysis of materials, and material analysis by electromagnetic means, etc., can solve the problems of difficult to achieve large-area ordered vertical pore preparation and small adjustable range of pore diameter. , to reduce degradation and non-specific molecular interference, reduce interference, and achieve a wide range of applications

Active Publication Date: 2018-08-10
HANGZHOU WELL HEALTHCARE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mesoporous silica is usually prepared by the micellar template method, the pore diameter can be adjusted in a small range, and it is difficult to realize the preparation of large-area ordered vertical channels.

Method used

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  • Preparation method and application of porous silicon material for biological sample pretreatment
  • Preparation method and application of porous silicon material for biological sample pretreatment
  • Preparation method and application of porous silicon material for biological sample pretreatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Fix the P-type boron-doped crystal silicon wafer in the electrolytic cell, add 3ml of ethanol and 40% hydrofluoric acid with a volume ratio of 1:0.05-1:6 as the electrolyte, and use the silicon wafer as the Anode, platinum electrode as cathode, conduct DC electrolytic etching and stripping, set current intensity at 20-50mA cm2, etching time at 1-4min, rinse the porous silicon layer after etching with ethanol, ultrasonically pulverize for 5min, and vacuum dry Porous silicon microparticles with a pore diameter of 5-15 nm, a thickness of 1-10 μm, and a diameter of 10-50 μm are obtained.

[0035]2) Soak the porous silicon particles in 1) in a mixed solution of 10% mass fraction of 3-butenoic acid and toluene solvent, heat and reflux for 2 hours, then soak and wash with ethanol, and obtain 3-butenoic acid after vacuum drying Modified porous silicon materials.

[0036] 3) Soak the porous silicon particles in 1) in a mixed solution of N,N dimethylallylamine and toluene so...

Embodiment 2

[0043] In this example, a porous silicon material with a porosity of 50.4% modified by 3-butenoic acid is used as the enrichment material to remove the high peak protein in human serum, and the specific steps are as follows:

[0044] 1), sample adsorption

[0045] Human serum was diluted 10 times with deionized water as the test solution.

[0046] Adsorption: 10 mg of porous silicon material with a porosity of 50.4% modified by 3-butenoic acid was added to 30 μL of the solution to be tested, and incubated together for 1 h at room temperature with shaking. The above suspension was left to stand for 2 min, and after the porous silicon particles settled to the bottom of the centrifuge tube, the supernatant was removed with a pipette gun.

[0047] Washing: add 100 μL of washing buffer (PBS buffer at pH 7.4) to the centrifuge tube, vortex and shake, let the suspension stand for 2 min, and carefully remove the supernatant with a pipette gun. Add 100 μL of deionized water to the ce...

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Abstract

The invention discloses a preparation method of a porous silicon material for biological sample pretreatment. The method comprises the following steps of (1) preparing porous silicon microparticles; (2) performing surface chemical modification on the porous silicon microparticles, wherein the porous silicon materials with the modification groups on the pore passage surfaces have micron scale particles, high-density nanometer scale vertical pore passages are contained inside, and modification groups are formed on the pore passage surfaces. The porous silicon materials for biological sample pretreatment is used for separating and enriching protein, polypeptide and nucleic acid; the protein, polypeptide and nucleic acid information is obtained through being combined with mass spectrometric detection, PCR or electrophoresis detection; the disease diagnosis, tumor mark discovery and biomolecule analysis are realized. When the porous silicon material for biological sample pretreatment is used for treating complicated biological samples, the high-molecular-weight high-abundant protein can be effectively eliminated; the target biological molecules can be selectively captured according to the molecular weight range, the charge features and the affine features; the detection sensitivity of the target biological molecules is further improved.

Description

technical field [0001] The invention belongs to the field of new materials and biotechnology and the field of mass spectrometry detection technology, and in particular relates to a preparation method and application of a porous silicon material used for biological sample pretreatment. Background technique [0002] At present, biological sample pretreatment technologies mainly include liquid chromatography separation, dialysis, centrifugation, extraction and other technologies. The above-mentioned technologies have disadvantages such as cumbersome operation process, high cost, poor effect, and easy loss and degradation of biomolecules. Currently commonly used biological sample pretreatment materials include carbon materials, magnetic beads, etc. Magnetic beads have the advantage of easy separation. However, when removing high-abundance proteins, a high amount of magnetic beads is required; when enriching low-abundance proteins, it is significantly affected by the affinity of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/447G01N27/62
CPCG01N1/28G01N27/447G01N27/62
Inventor 邬建敏臧运东陈晓明王涛栾春燕
Owner HANGZHOU WELL HEALTHCARE TECH CO LTD