Cordyceps militaris polysaccharide micro-capsules and preparation method thereof
A technology of Cordyceps militaris and microcapsules, applied in the field of medicine, can solve the problems of decreased immunity, irregular life, unreasonable diet, etc., and achieve the effect of low cost, large profit margin, and promoting adrenal glands
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Embodiment 1
[0030] 1. Wash, dry, pulverize Cordyceps militaris, reflux, concentrate, remove protein by Sevag method, centrifuge and other steps to extract polysaccharides from Cordyceps militaris, and precipitate with 4 times ethanol to obtain 2% sulfuric acid group content, 6% uronic acid content, weight A polysaccharide from Cordyceps militaris with an average molecular weight of 750kDa.
[0031] 2. Concentrate the microbial fermentation liquid until the total number of bacteria reaches 5×10 8 cfu / mL.
[0032] 3. Mix the concentrated microbial fermentation broth in step 2 with diatomaceous earth and polyethylene glycol at a ratio of 8% (w / v) and 5% (w / v) respectively, add the polysaccharide from Cordyceps militaris, and homogenize , form the militaris polysaccharide bacterium liquid, wherein the concentration of the militaris polysaccharide is 9g / L.
[0033] 4. Heat up the system in step 3 to 40°C, and mix the militaris militaris polysaccharide solution and the cross-linking salt solu...
Embodiment 2
[0035] 1. Wash, dry, crush, reflux, concentrate, remove protein by Sevag method, centrifuge and other steps to extract the polysaccharide from Cordyceps militaris, and precipitate with 5 times ethanol to obtain a sulfate group content of 3%, an uronic acid content of 7%, and a weight A polysaccharide from Cordyceps militaris with an average molecular weight of 850kDa.
[0036] 2. Concentrate the microbial fermentation liquid until the total number of bacteria reaches 5.5×10 9 cfu / mL.
[0037] 3. Mix the concentrated microbial fermentation liquid in step 2 with diatomaceous earth and polyethylene glycol at a ratio of 8% (w / v) and 5% (w / v) respectively, add the polysaccharide of Cordyceps militaris, homogenize, and form The polysaccharide bacterium liquid of Cordyceps militaris, wherein the concentration of the polysaccharide of Cordyceps militaris is 10g / L.
[0038]4. Heat up the system in step 3 to 40°C, and mix the militaris polysaccharide solution and the cross-linking sal...
Embodiment 3
[0040] 1. Wash, dry, pulverize Cordyceps militaris, reflux, concentrate, remove protein by Sevag method, centrifuge and other steps to extract polysaccharides from Cordyceps militaris, and precipitate with 6 times ethanol to obtain a sulfate group content of 4%, an uronic acid content of 8%, and a weight A polysaccharide from Cordyceps militaris with an average molecular weight of 950kDa.
[0041] 2. Concentrate the microbial fermentation broth until the total number of bacteria reaches 6×10 10 cfu / mL.
[0042] 3. Mix the concentrated microbial fermentation liquid in step 2 with diatomaceous earth and polyethylene glycol at a ratio of 8% (w / v) and 5% (w / v) respectively, add the polysaccharide of Cordyceps militaris, homogenize, and form The polysaccharide bacterium liquid of Cordyceps militaris, wherein the concentration of the polysaccharide of Cordyceps militaris is 11g / L.
[0043] 4. Warm up the system in step 3 to 40°C, and mix the polysaccharide solution of Cordyceps mi...
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