Liquid culture device for plant cells and organs

Abstract

The invention provides a simple liquid culture device for plant cells and organs. The simple liquid culture device comprises a hollow culture tank body, an inoculation port, an air inlet and an exhaust opening, wherein the inoculation port and the air inlet are formed in one end of the culture tank body; the exhaust opening is formed in the other end of the tank body; one end of the tank body is aplane and the other end of the tank body is conical; the inoculation port is formed in the top end of a cone and is matched with a sealing cover. Compared with an existing culture device, the simpleliquid culture device provided by the invention has the advantages of simple operation, high operation repeatability, low cost, low risk and low bacterial contamination rate; the volume of the culturetank body is 2 to 100L; materials for the culture tank and main accessories are transparent materials which can resist high temperature and can directly perform sterilization; the capacity of a culture medium is stable and the culture process is visual; an air distributor is arranged in the air inlet and is obliquely arranged; by rotary air inlet, the requirement of oxygen dissolving of the culture medium can be met at low throughput, and the air supply cost and the loss of the culture medium are reduced; in addition, the equipment is equipped with a special collecting port for organs which are difficult to collect, such as adventitious roots.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a plant cell and organ liquid culture device suitable for large-scale production. Background technique [0002] With the rapid development of global society and economy, the sufficient supply of existing fossil resources such as petroleum and coal has become unsustainable, and it is difficult to support the high-level development goals of human society. The development of human society will change from relying on hydrocarbon resources such as fossil fuels to relying on starch, cellulose, polysaccharides, plants and microbial oils and other carbohydrate resources that can be continuously synthesized by solar energy. This transformation of energy and resource structure will bring great opportunities and challenges to the development of biotechnology and its industries. [0003] In terms of available plant resources, my country is one of the countries with the richest medicina...

AI Technical Summary

Problems solved by technology

However, there are still the following problems: 1) currently commonly used reactors are all fixed at a certain position, and open-air inoculation is required during inoculation, and the tank body needs to be opened twice when feeding at the same time, the probability of bacterial contamination will increase significantly, and the problem of bacterial contamination is The most difficult problem to solve in plant tissue culture; 2) Reactors are mostly tanks made of stainless steel, so one or more transparent observation ports need to be set up during production

However, the wave wave reactor uses composite materials. The plastic bag used as the fermentation tank is composed of three layers of plastics. The inner layer is LDPE, the middle is EVOH, and the outermost layer is EVA. It cannot be autoclaved. The culture bag needs to be sterilized by irradiation first, and then the culture medium is sterilized separately and then poured into the culture bag. If feeding is needed in the middle, the culture bag needs to be reopened for operation. Adventitious root cultures are difficult to cultivate in the wave reactor. , and the culture bag cannot be reused, so it will cause environmental pollution. In addition, the oxygen in the medium of the wave culture bag is exchanged through the liquid surface gas, so the dissolved oxygen in the medium is likely to be insufficient, and the growth of the culture in the later stage will be severely restricted. Ultimately lead to issues with culture quality and yield

[0010] In order to solve the problem that the equipment in the prior art is expensive and complicated to operate, the culture medium evaporates a lot during the culture process, a large number of air bubbles are generated during the culture process, the feeding equipment, the culture medium and the culture equipment are sterilized separately, and the problem of increasing bacterial contamination when feeding, The present invention intends to provide an incubator that can meet the demand for dissolved oxygen under low aeration, the feeding equipment can be sterilized together with the cultivation equipment, and the bubble formation rate is lower during the cultivation process

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