Nanoprobe for photoacoustic rate bioassay of hydrogen sulfide, and manufacture method and application thereof

Abstract

The invention discloses a nanoprobe for photoacoustic rate bioassay of hydrogen sulfide, and a manufacture method and application thereof. The structural formula of the nanoprobe is shown as the formula I. The manufacture method of the nanoprobe mainly includes subjecting a raw material, Cy7-Cl, to nucleophilic substitution, knoevenagel condensation and amino azide reaction to obtain the nanoprobe. The problems of scattering and tissue absorption of fluorescence signals of fluorescence probes in bioassay are solved; the prepared nanoprobe has the advantages of high photoacoustic-signal penetration depth, imaging of deep tissues, high response speed, high specificity and high rate detection accuracy, and can be applied to bioassay of the hydrogen sulfide, or detection of the hydrogen sulfide in water and food. The formula I is shown in the specification.

Description

technical field [0001] The invention belongs to the field of tumor marker detection and imaging, and in particular relates to a photoacoustic ratio nanoprobe for detecting hydrogen sulfide in a living body, a preparation method and application thereof. Background technique [0002] Hydrogen sulfide is a toxic gas with the smell of rotten eggs. Hydrogen sulfide is the third biosynthetic gas transmitter after nitric oxide and carbon monoxide. Hydrogen sulfide plays an important role in many biological processes, such as blood vessels Relaxation of smooth muscle, regulation of neurotransmission, regulation of insulin signaling, inhibition of inflammation, sensing of oxygen, etc.; it also has a protective effect on the heart and nerves in the human body, and is an effective neuromodulatory substance, H 2 Physiologically relevant concentrations of S range from nanomolar to millimolar levels. At physiological concentrations, H 2 S is involved in a series of physiological regulat...

AI Technical Summary

Problems solved by technology

[0003] At present, the most commonly used methods for detection and imaging of hydrogen sulfide are the methylene blue method and the sulfur ion selective electrode detection method. Their disadvantages are that both are destructive and require homogenization of samples. For the study of more detailed physiological functions of hydrogen sulfide , new detection methods are needed With the rapid development of non-destructive detection technologies such as fluorescent imaging fluorescent probes, many novel detection methods have emerged [a) Li, H.D.; Yao, Q.C.; Fan, J.L.; Jiang, N. ; Wang, J.Y.; Xia, J.; Peng, X.J.Chem.Commun.2015, 51, 16225-16228.b) C.Liu, J.Pan, S.Li, Y.Zhao, L.Y.Wu, C.E.Berkman, A.R. Whorton and M.Xian, Angew.Chem., IntEd., 2011, 50, 10327–10329c) H.Peng, Y.Cheng, C.Dai, A.L.King, B.L.Predmore, D.J.Lefer and B.Wang, Angew.Chem ., Int. Ed., 2011, 50, 9672–9675d) K. Sasakura, K. Hanaoka, N. Shibuya, Y. Mikami, Y. Kimura, T. Komatsu, T. Ueno, T. Terai, H. Kimura and T. Nagano, J. Am. Chem. Soc. 2011, 133, 18003–18005]

[0004] The main disadvantage of this type of method is that in live detection, the scattering of ultrasonic signals by biological tissues to physiological tissues of the human body is 3 to 4 orders of magnitude lower than that of general optical signals, which means that photoacoustic imaging can provide deeper imaging depth and higher imaging resolution

Images