Method for preparing tendon composite muscle decellularized material derived from natural tissue

Abstract

The invention discloses a method for preparing a tendon composite muscle decellularized material derived from natural tissue. The method comprises the following step: treating tendon composite muscletissue of any size with a PBS (Phosphate Buffer Solution) with a protease inhibitor, a PBS with KCl, a PBS with KI, a PBS with Triton X, a PBS with SDS (Sodium Dodecyl Sulfate) and a PBS with DNA (Deoxyribonucleic Acid) enzymes, thereby obtaining the tendon composite muscle decellularized material derived from natural tissue. By adopting the method, cells on tissue can be subjected to complete decellularization treatment which is gentle in condition, free of ECM (Extracellular Matrix) damage and rapid and stable. The obtained material not only is capable of simulating layered structures of natural tendon composite muscle to the maximum extent and relatively well retaining extracellular matrix components, but also has the advantages of very low immunogenicity, relatively good mechanical properties, and the like, can be applied to repairing clinical muscle damage and regression related diseases caused by various disease reasons.

Description

technical field [0001] The present invention mainly relates to the medical field for repairing and regenerating skeletal muscle tissue of the whole body, in particular to the preparation of a tendon composite muscle decellularized material derived from natural tissue. It retains the layered structure of the natural tendon compound muscle, extracellular matrix components, has strong mechanical properties, and the material can promote the repair and regeneration of muscle defects. Background technique [0002] Muscle injuries caused by trauma, unreasonable exercise, degenerative diseases, etc. are very common in clinical practice and seriously affect the quality of life of patients. The widespread application of tissue engineering has made some progress in overcoming this disease. Current research is mainly focused on preparing a simple muscle scaffold to induce muscle fiber regeneration, but the effect is not ideal. First: Under physiological conditions, muscle fibers are co...

AI Technical Summary

Problems solved by technology

The widespread application of tissue engineering has made some progress in overcoming this disease. Current research is mainly focused on preparing a simple muscle scaffold to induce muscle fiber regeneration, but the effect is not ideal.

First: Under physiological conditions, muscle fibers are connected to tendons or myofascia, and it is through the connection of muscles and tendons that a network structure is formed, and a single muscle fiber forms a sum of contractions to produce efficient movement, while a simple muscle scaffold lacks this structure

Second: The mechanical properties of simple muscle scaffolds are poor (maximum stress is 0.1-5Mpa), and they are easy to rupture under high tension of large abdominal defects or limb muscle defects, while the maximum stress level of tendon scaffolds is 30-1000Mpa, which can Provide stable mechanical support

[0003] Due to the complexity of the natural tendon compound muscle tissue, especially the junction between the two, few studies have been able to create a scaffold that completely simulates the natural tendon compound muscle structure, which cannot achieve good repair and regeneration of muscle defects

Decellularization technology can provide a potential solution. While removing immunogenic cellular components, decellularized scaffolds can better simulate the natural tissue structure and retain extracellular matrix components. Currently, decellularized scaffolds for tendon and muscle have not yet see the report

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