Special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation
The technology of Bacillus subtilis and compound amino acid is applied in the field of organic fertilizers and Bacillus subtilis compound fertilizers, which can solve the problems of reduced microbial activity, and achieve the effects of good fruit shape, increased yield, and good appearance and taste.
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Embodiment 1
[0040] Example 1: Preparation of Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation
[0041] (1) The fermentation culture of Bacillus subtilis whose strain number is AB 90008 is used to obtain a fresh fermentation liquid with a viable count ≥ 70 billion / ml;
[0042] (2) Accurately weigh 60g of Bacillus subtilis fermentation liquid, 20g of amino acid liquid and 1g of bean cake powder;
[0043] (3) Add the amino acid liquid and bean cake powder in step (2) to the fermentation liquid of Bacillus subtilis, adjust the pH value to 4.0-4.5, stir slowly until fully dissolved, and store in a vat sealed at room temperature. After the preparation is completed, seal and store for 3 months , the live bacteria count result was 2.6x10 10 per ml, and the fermented liquid was directly stored for 3 months, and after drip irrigation and transportation, the counting result was 1.9x10 9 The results show that the compound amino acid liquid fertilizer can effe...
Embodiment 2
[0044] Embodiment two: the preparation of the Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation
[0045] Add 25g of amino acid solution and 5g of bean cake powder to 70g of Bacillus subtilis fermentation broth, adjust the pH value to 4.2-4.3, and keep it sealed for 6 months after the preparation is completed. The viable count result is 1.6x10 10 per ml, and the fermentation broth was directly stored for 6 months, and after drip irrigation and transportation, the counting result was 1.4x10 9 The results show that the compound amino acid liquid fertilizer can effectively prolong the activity of Bacillus subtilis.
Embodiment 3
[0046] Embodiment Three: Preparation of Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation
[0047] Use a vertical air-lift stirring bioreactor to ferment with Bacillus subtilis as the fermentation strain. Fermentation conditions: 10% inoculum, temperature 35-36°C, speed 250-300rpm, culture for 36-48h, and the number of viable bacteria ≥ 700 100 million / ml yellow-brown fermentation broth.
[0048] Feed soybean meal, peanut meal, cottonseed meal and rapeseed meal are superfinely pulverized to more than 80 meshes and mixed to obtain bean cake powder. Configure amino acid liquid, the amino acid liquid formula is glutamic acid 3%, aspartic acid 3%, alanine 2%, sodium sulfate 2%, sodium chloride 2%, humic acid 10%, the balance is water, mix The pH of the liquid is an amino acid liquid of 6.
[0049] Add 40g of amino acid solution and 5g of bean cake powder to 80g of Bacillus subtilis yellowish-brown fermentation broth, adjust the pH value t...
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