Special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation

The technology of Bacillus subtilis and compound amino acid is applied in the field of organic fertilizers and Bacillus subtilis compound fertilizers, which can solve the problems of reduced microbial activity, and achieve the effects of good fruit shape, increased yield, and good appearance and taste.

Inactive Publication Date: 2018-09-04
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the fact that there is no special biological fertilizer used in the drip irrigation industry to prolong the activity of Bacillus subtilis, and the technical status of the obvious reduction in microbial activity, as well as the shortcomings and shortcomings of the existing microbial compound liquid fertilizer specially used in the drip irrigation industry, the present invention aims at Provide a method that can significantly improve the preservation activity of Bacillus subtilis organic fertilizer for drip irrigation, and a high-activity Bacillus subtilis compound liquid fertilizer specially used for drip irrigation produced by this method

Method used

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  • Special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation
  • Special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation
  • Special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: Preparation of Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation

[0041] (1) The fermentation culture of Bacillus subtilis whose strain number is AB 90008 is used to obtain a fresh fermentation liquid with a viable count ≥ 70 billion / ml;

[0042] (2) Accurately weigh 60g of Bacillus subtilis fermentation liquid, 20g of amino acid liquid and 1g of bean cake powder;

[0043] (3) Add the amino acid liquid and bean cake powder in step (2) to the fermentation liquid of Bacillus subtilis, adjust the pH value to 4.0-4.5, stir slowly until fully dissolved, and store in a vat sealed at room temperature. After the preparation is completed, seal and store for 3 months , the live bacteria count result was 2.6x10 10 per ml, and the fermented liquid was directly stored for 3 months, and after drip irrigation and transportation, the counting result was 1.9x10 9 The results show that the compound amino acid liquid fertilizer can effe...

Embodiment 2

[0044] Embodiment two: the preparation of the Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation

[0045] Add 25g of amino acid solution and 5g of bean cake powder to 70g of Bacillus subtilis fermentation broth, adjust the pH value to 4.2-4.3, and keep it sealed for 6 months after the preparation is completed. The viable count result is 1.6x10 10 per ml, and the fermentation broth was directly stored for 6 months, and after drip irrigation and transportation, the counting result was 1.4x10 9 The results show that the compound amino acid liquid fertilizer can effectively prolong the activity of Bacillus subtilis.

Embodiment 3

[0046] Embodiment Three: Preparation of Bacillus subtilis compound amino acid liquid fertilizer specially used for drip irrigation

[0047] Use a vertical air-lift stirring bioreactor to ferment with Bacillus subtilis as the fermentation strain. Fermentation conditions: 10% inoculum, temperature 35-36°C, speed 250-300rpm, culture for 36-48h, and the number of viable bacteria ≥ 700 100 million / ml yellow-brown fermentation broth.

[0048] Feed soybean meal, peanut meal, cottonseed meal and rapeseed meal are superfinely pulverized to more than 80 meshes and mixed to obtain bean cake powder. Configure amino acid liquid, the amino acid liquid formula is glutamic acid 3%, aspartic acid 3%, alanine 2%, sodium sulfate 2%, sodium chloride 2%, humic acid 10%, the balance is water, mix The pH of the liquid is an amino acid liquid of 6.

[0049] Add 40g of amino acid solution and 5g of bean cake powder to 80g of Bacillus subtilis yellowish-brown fermentation broth, adjust the pH value t...

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Abstract

The invention discloses special high-activity bacillus subtilis compound amino acid liquid fertilizer for drip irrigation. The special high-activity bacillus subtilis compound amino acid liquid fertilizer for the drip irrigation is prepared from the following components in parts by weight: 60 to 80 parts of bacillus subtilis fermentation solution, 20 to 40 parts of amino acid liquid and 1 to 5 parts of soybean cake meal; the pH (Potential of Hydrogen) value of the compound amino acid liquid fertilizer is 4.0 to 4.5. According to the compound amino acid liquid fertilizer applicable to the dripirrigation, abundant small molecular nitrogen sources in an amino acid fermentation byproduct are sufficiently utilized; the compound amino acid liquid fertilizer is green and organic and is easily absorbed by crops; the living bacteria count is greater than or equal to 5 hundred million / milliliter, the content of organic matters is greater than or equal to 10 percent and the yield is increased by10 percent or more; relatively high activity is kept within 1 year; materials are very fine and smooth and the utilization rate of the fertilizer is improved; the fertilizer has dual effects of fertilizer promotion and biological control, a dominant position of root probiotics of crops is improved and soil borne diseases are inhibited; the occurrence of root diseases of the crops is reduced; thefertilizer belongs to green and environment-friendly type liquid-state biological organic fertilizer; a production process is green and environmentally friendly, and firm material guarantees are provided for organic agricultural ecological construction and sustainable development.

Description

technical field [0001] The invention relates to the technical field of organic fertilizers, in particular to the technical field of a bacillus subtilis compound fertilizer. Background technique [0002] Due to the need for agricultural water saving, drip irrigation technology has developed rapidly in recent years. Various forms of drip irrigation systems have appeared in field and facility agriculture, and fertilization with water is timed and fixed, accurate and efficient, and saves a lot of labor input costs. Therefore, drip irrigation Fertilizer has a broad market space in the future. With the green development of agriculture, the improvement of quality and efficiency, and the slogan of two reductions and one increase, there are more and more organic and inorganic compound drip irrigation liquid fertilizers on the market, many of which claim to add a variety of plant probiotics, and microbial activity is a measure of liquid The key indicator of the quality of bio-organic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05F17/00C12N1/20C12R1/125C05G3/80
CPCC05C11/00C05F17/00C05G3/00C05G3/80C12N1/20C05F5/002C05F11/08Y02W30/40
Inventor 陈竞杨新平秦新政王志方代金平冯蕾张慧涛买尔哈巴·艾合买提
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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