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Helicobacter pylori drug allergy detection culture medium and kit

A Helicobacter pylori culture medium technology, applied in the field of Helicobacter pylori drug susceptibility detection medium and kits, can solve the problems of lack of Helicobacter pylori drug susceptibility detection, troublesome operation, inaccurate measurement, etc., and achieve low cost and easy operation Easy, fast, and reasonably sized effects

Pending Publication Date: 2018-09-04
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such operations are not only cumbersome, inaccurate, and time-consuming, but more importantly, there is still a lack of standard culture media that can be used for drug susceptibility testing of Helicobacter pylori.

Method used

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  • Helicobacter pylori drug allergy detection culture medium and kit
  • Helicobacter pylori drug allergy detection culture medium and kit
  • Helicobacter pylori drug allergy detection culture medium and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 drug resistance detection card

[0044] The base of the detection card is a circular card, and an indicator ring is set on the surface of the card;

[0045] The diameter of the card is 10cm, and there are 4 indicator rings, and the 4 indicator rings do not overlap each other.

[0046] Each indicator ring is composed of two concentric circles and their centers, and the diameters of the concentric circles are 1.5cm and 2.4cm respectively.

[0047] The small circles in the concentric circles are marked as M circles, and the large circles are marked as S circles.

Embodiment 2

[0048] Embodiment 2 drug resistance detection petri dish

[0049] The base of the detection tool is the bottom of a circular petri dish, and an indicator ring is set on the surface;

[0050] The diameter of the petri dish is 10cm, and 4 indicator rings are set, and the 4 indicator rings do not overlap each other.

[0051] Each indicator ring is composed of two concentric circles and their centers, and the diameters of the concentric circles are 1.5cm and 2.4cm respectively.

[0052] The small circles in the concentric circles are marked as M circles, and the large circles are marked as S circles.

Embodiment 3

[0053] Example 3 culture medium

[0054] Prepare the culture medium according to the formula in Table 1:

[0055] Table 1 medium formula

[0056]

group 1

group 2

group 3

beef heart extract

250mL / L;

250mL / L;

250mL / L;

Bovine Brain Extract

200mL / L;

200mL / L;

200mL / L;

cysteine

1g / L;

5g / L;

2g / L;

Na 2 HPO 4

12g / L;

10g / L;

10g / L;

Peptone

4g / L;

8g / L;

5g / L;

NaCl

3g / L;

0.5g / L;

1g / L;

agar powder

20g / L.

30g / L.

25g / L.

[0057] Beef heart extract, bovine brain extract, cysteine, Na 2 HPO 4 , peptone, NaCl, add distilled water to 1000mL, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2±0.1, pack 800mL / bottle, autoclave in a high-pressure steam sterilizer, sterilize at 121°C for 20min , cooled to room temperature, sealed, and stored at 4°C for later use.

[0058] Take a certain amount of nutrient solutio...

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Abstract

The invention provides a culture medium for testing helicobacter pylori drug allergy and a determination method. The culture medium is unique in formula, can efficiently and stably promote the growthof helicobacter pylori, ensures that an inhibition zone in a drug allergy test detection result and a size of the inhibition zone are only related to the type of antibiotics and concentration of the antibiotics, so that the sensitivity of different helicobacter pylori isolate strain for different antibiotics can be scientifically determined by virtue of a set determination method and standard according to the presence and the size of the inhibition zone, and the helicobacter pylori drug allergy detection culture medium has the characteristics and advantages of rapidness, accuracy, intuitiveness and convenience. The helicobacter pylori drug allergy detection culture medium and kit are a unique and reliable novel drug allergy detection method for various pathogenic bacteria. Compared with the standard drug allergy test, the detection accuracy can reach 100 percent.

Description

technical field [0001] The invention relates to the field of drug sensitivity detection, in particular to a Helicobacter pylori drug sensitivity detection medium and a kit. Background technique [0002] Helicobacter pylori (Helicobacter pylori, HP) is a Gram-negative bacterium that can colonize the gastric mucosa, and the infection rate is as high as 50% to 70% worldwide. Epidemiological analysis showed that the detection rate of HP was 92.43% in chronic active gastritis, 89.47% in duodenal ulcer, 83.35% in gastric ulcer and 58.99% in gastric cancer. It can be seen that Helicobacter pylori infection is the main cause of chronic gastritis. At the same time, long-term HP infection can also cause severe atrophic gastritis, concomitant intestinal metaplasia, and even greatly increase the risk of gastric cancer. [0003] At present, the infection of Helicobacter pylori is mainly treated by antibiotic-based triple or quadruple therapy. However, the clearance rate of Helicobacter...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/045G01N2333/195
Inventor 王洪涛马伟民张永顶刘谦
Owner SHENZHEN BLOT BIOTECH
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