Method of making soy sauce by reusing and fermenting soy sauce residues
A technology of soy sauce and soy sauce, applied in the field of fermentation, can solve the problems of low degree of deep processing and reuse, and low added value of products, and achieve the effects of increasing added value of products, improving raw material utilization, and increasing nutrient content
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[0010] 1. Preparation stage
[0011] 1) Prepare and sterilize the petri dish, shaker and fermenter for use;
[0012] 2) Inoculate Aspergillus oryzae, Pleurotus ostreatus, and Ganoderma lucidum (10% inoculum) into PDA medium containing 30% soy sauce residue and cultivate them at 30° C. for 3 days, and put them in the refrigerator for later use after cultivation;
[0013] 3) Prepare protease, peptidase, amylase, glutaminase, pectinase, cellulase, and hemicellulase in advance, and put them in the refrigerator for later use;
[0014] 4) Inoculate the cultured Aspergillus oryzae, Oyster mushroom, and Ganoderma lucidum strains into 100ml of liquid culture medium at 30°C and 200rpm for 2 days on a shaker, and put them in the refrigerator for later use;
[0015] The ingredients of the liquid medium are: 3g of beef extract, 10g of peptone, 10g of corn flour, 20g of soy sauce residue, 10g of distiller’s grains, 5g of sodium chloride, pH7.0-7.2, add water to 1000ml, and autoclave at 121...
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