Specific fluorescence labeling method of food-borne probiotics and in-vivo application thereof

A technology of fluorescent labeling and probiotics, applied in the research field of food-derived probiotics, can solve the problems of unstable luminous intensity, lack of optical stability, coherent light excitation, etc., and achieve excellent afterglow performance, good photochemical stability, and fluorescence long life effect

Inactive Publication Date: 2018-09-14
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these materials have their own defects to varying degrees, such as poor signal-to-noise ratio, lack of optical stability, photobleaching, coherent photoexcitation, unstable luminous intensity, etc.

Method used

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  • Specific fluorescence labeling method of food-borne probiotics and in-vivo application thereof
  • Specific fluorescence labeling method of food-borne probiotics and in-vivo application thereof
  • Specific fluorescence labeling method of food-borne probiotics and in-vivo application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A specific fluorescent labeling method for food-borne probiotics

[0042] (1) Preparation of Cr with near-infrared fluorescence and ultra-long afterglow properties by solvothermal-high-temperature calcination method 3+

[0043] Doped zinc gallogenate long-lasting fluorescent nanoprobes.

[0044] Accurately weigh 0.013mmol Cr(NO 3 ) 3 9H 2 O and 3.01mmol Zn(NO 3 ) 2 ·6H 2 O is uniformly dissolved in 0.6mol L prepared in 10mL -1 Ga(NO 3 ) 3 Stir magnetically in the solution to make it evenly mixed, adjust the pH to 7.5 with tert-butylamine for co-precipitation, continue magnetic stirring for 2 hours, and ultrasonically treat for 30 minutes to make it evenly mixed, and place the formed white emulsion in a polytetrafluoroethylene reaction kettle for 120 ℃ hydrothermal reaction for 24h. Take it out and let it cool down to room temperature naturally, add it to 2 times the volume of ethanol, mix evenly, produce a precipitate, centrifuge at 7000rpm for 10min to colle...

Embodiment 2

[0048] A method for specific fluorescent labeling of food-derived probiotics, the steps and method of which are basically the same as in Example 1, except that the probiotics are Bifidobacterium lactis V9, and correspondingly fluorescently labeled Bifidobacterium lactis V9 is obtained.

Embodiment 3

[0050] A specific fluorescent labeling method for food-derived probiotics, the steps and method are basically the same as those in Example 1, the difference is that the probiotics are Lactobacillus reuteri, and fluorescently labeled Lactobacillus reuteri is obtained accordingly.

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Abstract

The invention relates to a specific fluorescence labeling method of food-borne probiotics. The method includes following steps: (a), preparing a Cr3+doped gallium zinc germanate long-afterglow fluorescent nano probe having near infrared fluorescence and ultralong-afterglow properties through a solvothermal-high-temperature calcining process; (b), realizing specific fluorescence labeling of the nano probe to target probiotics through antibody surface functionalizing. The method has the advantages that the nano probe prepared by the method has ultrastrong near infrared luminescence, ultralong afterglow life, excellent biocompatibility and structural stability, high grain size uniformity and low toxicity; near infrared fluorescence labeling probiotics in-vivo biological imaging technology developed by the invention can realize nondestructive tracing of distribution of probiotics after entering organisms, and the method is of important significance in developing functional sites of innovative food-borne probiotics and nutriology research concepts.

Description

technical field [0001] The invention belongs to the research field of food-derived probiotics, and relates to a specific fluorescent labeling method of food-derived probiotics and its application in vivo. Background technique [0002] Probiotics, which are an important part of the gut microflora, can bring great benefits to the health of consumers (hosts) when ingested in sufficient quantities. Probiotics can greatly promote the health of the human intestinal tract and regulate the immune response of the human body. Although it has great benefits and special functions to human health, the metabolism, distribution and immune regulation related to probiotics in the body are still not very clear, and the functions and activities of most new species are still not clear, and more research is needed. more information to evaluate. [0003] At present, modern molecular biology techniques such as polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE), real...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C09K11/66
CPCA61K49/0058A61K49/0019A61K49/0067C09K11/68
Inventor 刘敬民王硕
Owner NANKAI UNIV
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