Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining soil microbial biomass phosphorus

A soil microorganism and determination method technology, applied in the field of microbial phosphorus determination, can solve the problems of low soil microbial phosphorus content, inaccurate experimental results, cumbersome experimental process, etc., and achieve accurate detection results, improve oxidation rate, and avoid safety accidents. Effect

Inactive Publication Date: 2018-09-18
QINGHAI UNIVERSITY
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional method uses sulfuric acid-hydrogen peroxide-perchloric acid for digestion. The experimental process of this traditional method is cumbersome and time-consuming. At the same time, due to the low phosphorus content of soil microorganisms, the digestion process easily leads to phosphorus loss, which will make the experimental results inaccurate or even negative. value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining soil microbial biomass phosphorus
  • Method for determining soil microbial biomass phosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A method for determining soil microbial biomass phosphorus, including the following steps:

[0060] (1) Divide the soil samples into two groups, where the first group of samples were fumigated with chloroform, and after removing the chloroform, sodium bicarbonate was added for extraction and then filtered to obtain the first filtrate; the second group of samples were directly added to the After leaching with sodium bicarbonate, filter the second filtrate.

[0061] (2) After adjusting the first filtrate and the second filtrate to neutral, add potassium persulfate, and react under high temperature and high pressure for 10-25 min (for example, 10 min, 15 min, 20 min or 25 min).

[0062] (3) Determine the phosphorus content of the first filtrate and the second filtrate after the reaction, and the difference between the phosphorus content of the first filtrate and the phosphorus content of the second filtrate is the value of microbial biomass phosphorus.

Embodiment 2

[0064] A method for determining soil microbial biomass phosphorus, including the following steps:

[0065] (1) Divide the soil samples into two groups, among which, the first group of samples are treated with chloroform fumigation, and after removing the chloroform, add the concentration of 0.45-0.55mol / l (for example: 0.45mol / l, 0.50mol / l or 0.55 mol / l) of sodium bicarbonate solution at a temperature of 20-25℃ (for example: 20℃, 23℃ or 25℃) and an oscillation speed of 180-220r / min (for example: 180r / min, 200r / min or 220r / min) under the condition of shaking for 25-35min (for example: 25min, 30min or 35min), filter with non-phosphorus filter paper to obtain the first filtrate, where the mass ratio of the mass of the soil of each sample to the mass of sodium bicarbonate solution is 1:( 8-12) (for example: 1:8, 1:10, or 1:12).

[0066] Add sodium bicarbonate with a concentration of 0.45-0.55mol / l (for example: 0.45mol / l, 0.50mol / l or 0.55mol / l) to the second group of samples (divide...

Embodiment 3

[0070] A method for determining soil microbial biomass phosphorus, including the following steps:

[0071] (1) Reagent configuration: The configuration method of molybdenum antimony sulfate solution is: take 17ml concentrated sulfuric acid, slowly add to 100ml distilled water, cool, add 1.2g ammonium molybdate tetrahydrate to make it completely dissolved, add 10ml 0.3% tartaric acid Antimony potassium solution, mix well, distilled water to dilute the volume to 1000ml, this solution can be stored at room temperature for 1 week.

[0072] The preparation method of ascorbic acid solution is as follows: 2.5g ascorbic acid is dissolved in distilled water, and the volume is fixed to 100ml. It is stored in a brown bottle and is effective within 24 hours.

[0073] The configuration method of 33% sulfuric acid solution is as follows: Dissolve 227.8ml concentrated sulfuric acid in about 800ml distilled water, and cool and dilute to 1000ml.

[0074] The configuration method of 5% potassium persul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for determining soil microbial biomass phosphorus, and belongs to the technical field of microbial phosphorus determination. The method for determining the soil microbial biomass phosphorus comprises the following steps: soil samples are divided into two groups, a first group of samples is subjected to chloroform fumigation treatment, and after removing of chloroform, sodium hydrogen carbonate is added for leaching, and a first filtrate is filtered; the sodium hydrogen carbonate is directly added into a second group of samples for leaching, and a second filtrateis filtered; the first filtrate and the second filtrate are adjusted to neutral, potassium persulfate is added, reaction is performed under high temperature and high pressure for 10-25 min; phosphorus contents of the first filtrate and the second filtrate after the reaction are measured, and a difference between the phosphorus contents of the first filtrate and the phosphorus content of the second filtrate is the value of the microbial biomass phosphorus. The method for determining the soil microbial biomass phosphorus is simple, the measurement time is short, and the amount of phosphorus loss during experiments is reduced.

Description

Technical field [0001] The present invention relates to the technical field of determination of microbial phosphorus, in particular to a method for measuring soil microbial biomass phosphorus. Background technique [0002] Phosphorus is an essential nutrient element for microorganisms and a constituent element of microbial cell components. Phosphorus compounds in microbial cells mainly include ribonucleic acid (30%-50%), sugar esters and nucleoside esters, various phosphorylated coenzymes, acid-soluble inorganic and organic polyphosphates (15%-20%), phospholipids (< 10%), deoxyribonucleic acid (5%-10%) and a small amount of inositol phosphate. Soil microbial biomass P (soilmicrobial biomass P) refers to the phosphorus contained in all living microorganisms in the soil, which usually accounts for 1.4%-4.7% of the mass of microbial dry matter. The rapid turnover of soil microbial biomass phosphorus plays an important role in the cyclic transformation of soil inlay phosphorus a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 李世雄尹亚丽王玉琴刘燕马玉寿王彦龙赵文
Owner QINGHAI UNIVERSITY