Biological sensing three-dimensional chip and preparation method thereof

A biosensing, three-dimensional chip technology, applied in the field of biological analysis, can solve the problems of low sample load, limited diversity and application of three-dimensional biosensing chip, weak specificity, etc. Controlled, specific effects

Active Publication Date: 2018-09-28
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most biosensing chips are two-dimensional biosensing chips, that is, combining a single-molecule self-assembly layer on the surface of the sensor chip can realize the basic functions of the biosensing chip, but it has low sample loading, Disadvantages of less specificity
[0004] In order to overcome these problems, the development and application of three-dimensional sensor chips has become a trend. However, the current preparation methods of three-dimensional biosensor chips are all based on the "graft from" technology, and the preparation methods are relatively single to a certain extent. This limits the diversity and application of 3D biosensing chips

Method used

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  • Biological sensing three-dimensional chip and preparation method thereof
  • Biological sensing three-dimensional chip and preparation method thereof
  • Biological sensing three-dimensional chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Put the glass substrate coated with gold film in a plasma cleaner for 10 minutes, and soak the cleaned gold-coated glass substrate in an ethanol solution with a total concentration of 1 mM anchoring agent (molecular weight is about 400) , driven by self-assembly, a self-assembled monomolecular anchor layer is formed on the surface of the substrate;

[0039] (2) soak the substrate containing the self-assembled monomolecular anchoring layer obtained in step (1) in the ethyl acetate solution of 3-bromopropyne, react at room temperature for 6 hours, clean the chip, and obtain the surface alkynyl functional group Modified substrates;

[0040] (3) In a reaction flask, add 0.01 molar portion of ethyl bromoisobutyrate, 1 molar portion of polyethylene glycol methacrylate monomer and 1 molar portion of hydroxyethyl methacrylate monomer, 0.01 molar parts of copper chloride, 0.01 molar parts of sodium ascorbate, 100 molar parts of methanol and 100 molar parts of deionized wate...

Embodiment 2

[0045] (1) Put the silver-coated glass substrate in a plasma cleaning machine to clean for 10 minutes, soak the cleaned gold-plated glass substrate in an ethanol solution of anchoring agent (molecular weight is about 1000) with a total concentration of 10mM , driven by self-assembly, a self-assembled monomolecular anchor layer is formed on the surface of the substrate;

[0046](2) soak the substrate containing the self-assembled monomolecular anchoring layer obtained in step (1) in a dimethyl sulfoxide (DMSO) solution of 6-bromohexyne, react at room temperature for 24 hours, and clean the chip. Obtain a substrate modified with alkynyl functional group on the surface;

[0047] (3) In a reaction bottle, add 0.02 molar parts of ethyl 2-bromoisobutyrate, 1 molar part of polyethylene glycol methacrylate monomer and 2 molar parts of hydroxyethyl methacrylate monomer, 0.03 molar parts of copper chloride, 0.03 molar parts of sodium ascorbate, 300 molar parts of methanol and 100 molar...

Embodiment 3

[0052] (1) Put the glass substrate coated with gold film in the plasma cleaning machine for cleaning for 10 minutes, soak the cleaned gold-plated glass substrate in the ethanol solution of anchoring agent (molecular weight is about 10000) with a total concentration of 5mM , driven by self-assembly, a self-assembled monomolecular anchor layer is formed on the surface of the substrate;

[0053] (2) Soak the substrate containing the self-assembled monomolecular anchoring layer obtained in step (1) in a tetrahydrofuran (THF) solution of 4-chloro-n-butyne, react at room temperature for 16 hours, clean the chip, and obtain the surface alkyne Substrates modified with functional groups;

[0054] (3) In a reaction flask, add 0.005 molar parts of ethyl 2-bromoisobutyrate, 1 molar part of polyethylene glycol methacrylate monomer and 3 molar parts of hydroxyethyl methacrylate monomer, 0.1 molar parts of copper chloride, 0.1 molar parts of sodium ascorbate, 200 molar parts of methanol and...

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Abstract

The invention relates to the field of biological analysis, and particularly relates to a biological sensing three-dimensional chip and a preparation method thereof. The biological sensing three-dimensional chip provided by the invention has a supporting layer, an anchoring layer, a click modifying layer and a high-molecular brush layer; the anchoring layer is anchored and bonded on the supportinglayer through Au-S bonds or Ag-S bonds; the click modifying layer is bonded on the anchoring layer through covalent bonds; and the high-molecular brush layer is covalently bonded to the click modifying layer by a clicking reaction. In a preparation process, according to the invention, the high-molecular brush layer is constructed by using a method of ''Grafted to'', so that the molecular weight ofthe high-molecular brush is well controlled, and the repeatability of the technical process for preparing the chip and the stability of the quality of the chip are improved. According to the invention, the method of ''Grafted to'' is realized through click chemistry, so that the biological sensing three-dimensional chip having the high-molecular brush layer with a high grafting density is prepared. The biological sensing three-dimensional chip finally prepared by the preparation method provided by the invention has the characteristics of high sample carrying amount and strong specificity.

Description

technical field [0001] The invention relates to the field of bioanalysis, in particular to a biosensing three-dimensional chip and a preparation method thereof. Background technique [0002] Biochip is the product of the Genome Project. It mainly builds a miniature biochemical analysis system on the surface of the solid chip through microprocessing technology and microelectronics technology to realize the detection of the sample to be analyzed, but because of its low sensitivity and insufficient specificity And other shortcomings, to a certain extent limit its application. Biosensor is one of the cutting-edge technologies of modern science and technology, and has become one of the important fields of modern biotechnology; while the biosensing chip combines the advantages of both biochip and biosensor technologies, which not only improves the detection efficiency, but also improves The sensitivity of the detection is improved and the specific recognition of the analysis samp...

Claims

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Application Information

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IPC IPC(8): C03C17/38C08F8/30C08F283/06C08F220/20
CPCC03C17/3644C03C17/3649C03C17/38C03C2217/255C03C2217/256C03C2218/111C08F8/30C08F283/065C08F220/20
Inventor 彭开美杨再波阮代锬王江河戴学新
Owner QIANNAN NORMAL UNIV FOR NATTIES
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