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Synthesis method and application of dual-wavelength fluorogold nanocluster and application

A technique for the synthesis of fluorescent gold nano-clusters, which is applied in the field of synthesis of dual-wavelength fluorescent gold nano-clusters, can solve problems such as no synthetic materials, and achieve good specificity

Active Publication Date: 2018-09-28
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the method of synthesizing gold nanoclusters using carbon quantum dots as reducing agent and stabilizer, and there is no report on the synthesis of materials for two kinds of fluorescent ratio probes

Method used

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  • Synthesis method and application of dual-wavelength fluorogold nanocluster and application
  • Synthesis method and application of dual-wavelength fluorogold nanocluster and application
  • Synthesis method and application of dual-wavelength fluorogold nanocluster and application

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: Determination of temperature and tryptophan content in blood The operation steps are as follows:

[0027] (1) Synthesis of carbon quantum dots: Weigh 2g of dry Dendrobium officinale powder, disperse it in 90mL of hot water at 60°C and keep stirring, and then add 10mL of absolute ethanol and 1mL of ethylenediamine after stirring until it becomes a paste. After stirring evenly, transfer to a polytetrafluoroethylene-lined reactor and heat at 180°C for 9 hours. After natural cooling, centrifuge at 8000 rpm for 20 minutes to remove large particles of impurities. The supernatant is passed through a 0.22 μm filter membrane to obtain nitrogen-doped carbon quantum Dots, using quinine sulfate as a standard substance, the fluorescence quantum yield is 25%; the obtained quantum dots are dried in a vacuum oven at 60°C for 24 hours, and set aside;

[0028] (2) Synthesize fluorescent gold nanoclusters using carbon quantum dots as reducing agent and protective agent: meas...

Embodiment 2

[0034] Embodiment 2: the content determination steps of temperature and tyrosine in the fermented liquid sample are:

[0035] (1) Synthesis of carbon quantum dots: Weigh 3g of dry Dendrobium officinale powder, disperse it in 110mL of hot water at 65°C and keep stirring, and then add 15mL of absolute ethanol and 2mL of ethylenediamine after stirring until it becomes a paste. After stirring evenly, transfer to a polytetrafluoroethylene-lined reaction kettle and heat at 180°C for 10 hours. After natural cooling, centrifuge to remove large particles of impurities. After the supernatant is passed through a 0.22 μm filter membrane, nitrogen-doped carbon quantum dots are obtained. The nitrogen-doped carbon quantum dots were vacuum-dried at 65°C for 24 hours, and set aside;

[0036] (2) Synthesize fluorescent gold nanoclusters using nitrogen-doped carbon quantum dots as a reducing agent and protective agent: take the nitrogen-doped carbon quantum dots in step (1), prepare an aqueous s...

Embodiment 3

[0040] Embodiment 3: the temperature and tyrosine content determination steps in the milk sample are:

[0041] (1) Synthesis of carbon quantum dots: Weigh 5g of dried Dendrobium officinale powder, disperse it in 150mL of hot water at 70°C and stir continuously, and then add 20mL of absolute ethanol and 3mL of ethylenediamine after stirring until it becomes a paste. After stirring evenly, transfer to a polytetrafluoroethylene-lined reaction kettle and heat at 180°C for 11 hours. After natural cooling, centrifuge to remove large particles of impurities. After the supernatant is passed through a 0.22 μm filter membrane, nitrogen-doped carbon quantum dots are obtained. The nitrogen-doped carbon quantum dots were vacuum-dried at 62°C for 24 hours, and set aside;

[0042] (2) Synthesize fluorescent gold nanoclusters using nitrogen-doped carbon quantum dots as a reducing agent and protective agent: take the nitrogen-doped carbon quantum dots in step (1), prepare an aqueous solution w...

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Abstract

The invention discloses a synthesis method of a dual-wavelength fluorescent gold nanocluster. The method comprises the following steps: taking dendrobium nobile rich in polysaccharide as a carbon quantum material, synthesizing a fluorescent carbon quantum dot through a one-step thermal decomposition method, taking the carbon quantum dot as a reducing agent and a protective agent for synthesizing the fluorescent gold nanocluster, and synthesizing the dual-wavelength fluorescent gold nanocluster. Under 360nm excitation wavelength, the fluorescent gold nanocluster produces emission spectrums in ablue light zone (443nm) and a red light zone (714nm) at the same time, and the fluorescent gold nanocluster is taken as a fluorescent probe for temperature and tyrosine and constructs a ratio fluorescence sensor; the 443nm blue light zone has linear response to temperature change, and the fluorescence intensity is gradually reduced during the process that the temperature is risen to 75 DEG C from5 DEG C, and fluorescence is recovered when the temperature is reduced; the tyrosine has a linear enhancement effect on the 714nm red light zone, and the fluorescence intensity is increased with theconcentration of the tyrosine, so that a ratio fluorescence probe for temperature and the tyrosine is constructed; the method has strong specificity and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a synthesis method and application of a dual-wavelength fluorescent gold nanocluster. Background technique [0002] Gold nanoclusters, composed of tens to hundreds of gold atoms, are fluorescent nanomaterials that have aroused great research interest of scientists in recent years, and their particle sizes are usually less than 2nm. Since its particle size is close to the Fermi wavelength of electrons, it has different optical, chemical and electrical properties from gold nanoparticles with large particle sizes. As a new type of fluorescent nanomaterial, gold nanocluster has the advantages of simple synthesis, strong photostability, and good biocompatibility. It has become one of the most promising materials in the field of nanotechnology. It is used in the fields of life medicine and biosensing. are widely used in. In the fluorescence sensing mode, the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58G01K11/32G01N21/64B82Y20/00B82Y40/00
CPCG01K11/32G01N21/6428C09K11/58B82Y20/00B82Y40/00G01N2021/6439
Inventor 杨亚玲华建豪
Owner KUNMING UNIV OF SCI & TECH