Bacillus pumilus s35 and application thereof

A technology of Bacillus pumilus and strains, applied in the field of microorganisms, can solve the problems of insufficient biological control technology of potato dry rot, and achieve the effects of low cost, good control effect, effective antagonism and control

Inactive Publication Date: 2018-09-28
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Bacillus pumilus (Bacillus.pumilus) obtained by separating and purifying from the mud of Chaerhan Salt Lake and its biocontrol application. The technical problem to be solved is aimed at the current biological control technology of potato dry rot lack of

Method used

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  • Bacillus pumilus s35 and application thereof
  • Bacillus pumilus s35 and application thereof
  • Bacillus pumilus s35 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and Identification of Bacillus pumilus S35

[0028] 1.1 Isolation and purification of Bacillus pumilus S35

[0029] 1) Water sample pretreatment: Under sterile conditions, the water sample was mixed evenly, filtered with a filter equipped with a filter membrane with a pore size of 0.22 μm, and bacterial cells were enriched on the filter membrane. When the amount of water sample used for enrichment reaches 30ml, remove the filter membrane and put it into a glass test tube filled with 3ml of sterile seawater for 10 -1 watery.

[0030] 2) Soil sample pre-treatment: Take an appropriate amount of soil sample, air-dry (about 20 days), and treat at 120°C for 1 hour. Weigh 10g of the treated soil sample, add 90ml of sterile seawater, put it into a sterilized Erlenmeyer flask equipped with glass balls, shake it fully for 30min, and draw the supernatant after standing still, the supernatant is 10 -1 Soil sample. the above 10 -1 Samples were serially dilut...

Embodiment 2

[0058] Antagonism of Example 2 Bacillus pumilus S35 to pathogenic fungi

[0059] 1.1 Bacteria culture

[0060] Inoculate 4 strains of pathogenic fungi (Qing 9A-4-13, Qing 9A-5-2, Qing 9A-5-10, 65B-2-6) into PDA medium, and culture them in a constant temperature incubator at 28°C for one week; Bacillus pumilus S35 was inoculated in medium E and cultured in a 37°C incubator for 1 day.

[0061] 1.2 Consistent training

[0062] Using the "cross-hatching method", the pathogenic fungus was punched into a bacterium cake with a diameter of 5 mm and placed at a distance of 2.5 cm from the center of Bacillus pumilus (colony diameter of 5 mm), and confronted culture was carried out in a petri dish with a diameter of 9 cm. The results are shown in Table 3.

[0063] Table 3 The confrontation culture between s35 and 4 strains of potato dry rot pathogenic fungi

[0064]

[0065] It can be seen from Table 3 that there was no significant difference between the colony radius and the widt...

Embodiment 3

[0066] Example 3 Re-screening of potatoes in vivo

[0067] 1.1 Preparation of fermentation broth

[0068] The bacteria cultivated in Example 2 were inoculated into an Erlenmeyer flask filled with sterile water, placed in a constant temperature shaker at 37° C. and 200 r / min, and cultured with shaking for 5 days before use.

[0069] 1.2 Preparation of bacterial suspension

[0070] Inoculate the bacteria cultured in Example 2 into a Erlenmeyer flask filled with sterile water, place it in a constant temperature shaker at 37°C and 200 r / min for 2 hours, shake well and set aside.

[0071] 1.3 Test method

[0072] Prick two diameters of 5mm and 3mm deep wounds on the sterilized potato fruit with a puncher with a diameter of 5mm (the potato fruit was soaked in 2% NaClO solution for 10min, rinsed with tap water, and dried naturally). Inoculate 40 μl of pathogenic bacteria suspension first, and then inoculate the same volume of Bacillus pumilus suspension. The control was first ino...

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Abstract

The invention discloses a bacillus pumilus S35 and application thereof. The bacteria strain has been preserved in Guangdong Microbiology institute Guangdong Microbial Strain Preservation Center, the address is F5, Building 59, Yard 100, Xianliezhonglu, Guangzhou) on September 12th, 2016 and has the preservation number of CGMCC NO. 60077. The bacterial strain has obvious antagonism for diseases caused by fusarium, and a bacterial suspension and secondary metabolites are preferably prevented, and the bacterial strain is wide in source of materials, low in cost and safe for environment.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a halophilic bacterium derived from Chaerhan Salt Lake and its biocontrol application, in particular to a Bacillus pumilus s35 and its application. Background technique [0002] Potatoes belong to the annual herbaceous plant of Solanaceae Solanaceae. They are rich in nutrients and contain high protein, vitamins and inorganic salts. With the launch of the potato staple food strategy in my country in 2015, the potato planting area continues to expand, and the occurrence of diseases is becoming more and more serious. Among them, Fusarium dry rot is one of the important diseases of potato during storage. It often leads to the decrease of potato tuber quality and the rate of commercial potato, seriously affecting its edible value and economic value, and has become the main factor restricting the development of potato industry. At present, chemical fungicides are mainly used to control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 沈硕李玮郭青云王舰他永全
Owner QINGHAI UNIVERSITY
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