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A method for determining the content of paraquat and diquat in biological fluid

A technology of paraquat and diquat, applied in the field of determination of bipyridyl compound content in human tissue, can solve the problems of high detection limit, difficult separation, difficult popularization and application, and achieve high sensitivity, strong anti-interference ability and low cost Effect

Active Publication Date: 2021-03-26
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paraquat and diquat have similar structures and are not easy to separate. At the same time, false positive results and false negative results are often caused by the interference of endogenous compounds. The LC-MS method has high selectivity and sensitivity, but often requires hydrophilic interaction. Functional chromatographic columns and instrument consumables are expensive to detect and maintain, making it difficult to popularize and apply them clinically

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  • A method for determining the content of paraquat and diquat in biological fluid
  • A method for determining the content of paraquat and diquat in biological fluid
  • A method for determining the content of paraquat and diquat in biological fluid

Examples

Experimental program
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Embodiment 1

[0023] Example 1 (Measurement Method Example)

[0024] Instruments, reagents and samples

[0025] 1.1. Instrument: Vortex-Genie adjustable vortex mixer; Shumei KQ-500DB type CNC ultrasonic cleaner; micropipetizer; Milli-q Element ultrapure water treatment system; 1 / 1000 electronic balance; G13U desktop centrifuge The LC-20A high performance liquid chromatograph (SPD-M20A diode array detector); Phenomenex Synergi MAX-RP 80A-C12 column (250 × 4.6 mm, 4 μm).

[0026] 1.2. Reagents: Triton X-114; NaCl (chromatographic pure); formic acid (chromatographic pure); sodium octane sulfonate (chromatographic pure); phosphoric acid; diethylamine; acetonitrile (chromatography pure).

[0027] Formulated 0.14 g / ml Triton X-114 solution: Take 100ml capacity bottle, use 1 / 1000 electronic balance to peeled, add 14G Triton X-114, set up to the scale line with ultrapure water, and shake and shake The -4 ° C refrigerator is refrigerated for 4 h and takes out to return to room temperature.

[0028] Th...

Embodiment 2

[0058] Example 2 (Example of Kit)

[0059] The kit of the present embodiment includes a reagent A, reagent B, reagent C, reagent D, reagent E, and reagent F, and the preparation method of each reagent is as follows.

[0060] Reagent A: 0.14 g / ml Triton x-114 solution; the preparation method is as follows, Triton X-114 14.0g, add pure water to 100 mL;

[0061] The reagent B: 0.1308 g / ml NaCl solution; the preparation method is as follows, NaCl 6.54 g, add pure water to 50 mL;

[0062] Reagent C: 5mL of formic acid;

[0063] Reagent D: 0.1% formic acid solution; the preparation method is as follows, 100 μL of formic acid, add pure water to 100 mL;

[0064] Reagent E: 5 mmol / l octane sulfonate (containing 0.4% phosphoric acid) 1000 mL; Preparation method, weighing 1.081 g of octane sulfonate in a 1000 mL volumetric flask, 4 ml of phosphoric acid, then add 4 ml of phosphoric acid after dissolving 200 mL The addition of diethylamine regulates the pH of 3.5, then the ultrasonic de...

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Abstract

The invention relates to a method for testing or analyzing a material by determining the chemical or physical properties of the material, in particular to a method for determining the contents of paraquat and diquat in biological fluid. The method comprises the steps: taking a fluid sample and sequentially adding a Triton X-114 aqueous solution, a NaCl aqueous solution and formic acid, so that thefinal concentration of Triton X-114 is 0.0545 to 0.109g / mL, the final concentration of NaCl is 0.0165 to 0.0495g / mL, and the final concentration of formic acid is 15 to 30 muL / mL; then oscillating, ultrasonically treating in a water bath, centrifuging, taking a supernatant, and filtering to obtain a sample injection solution of a sample to be determined; taking standard solutions of different concentrations of the same volume with the fluid sample to prepare a series of concentration standard product sample injection solutions; detecting the sample injection solution of the sample to be determined and a series of concentration standard product sample injection solutions by a liquid phase ion pair chromatography to obtain liquid phase chromatograms of the sample to be determined and standard products, the chromatograms thereof are compared to determine the characteristic peak, and then the content of paraquat or diquat in the sample is detected by calculation of a calibration curve according to the peak area.

Description

Technical field [0001] The present invention relates to tested or analyzing materials by means of chemical or physical properties of the measurement material, and more particularly to a method of determining the content of lipidridine compound in a human tissue. Background technique [0002] Paraquat and enemy grass (Diquat) belong to a bobium compound, belonging to non-selective trocar type herbicides, a strong human, animal toxic, currently no special treatment method, my country's paraquat poisoning patient mortality has been Up to 85% ~ 90%. Studies have shown that paraquat and enemy grass can be used to enter the human body through the skin, mucous membrane, respiratory tract, and digestive tract, in the heart, liver, brain and kidney and other organs are distributed, of which the lung part is most, the maximum damage is maximum, pulmonary fibrosis Respiratory failure is the main reason for the death of patients in patients. Modern medicine generally approves the patient pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14
Inventor 潘加亮袁嘉豪黄清湄马安德
Owner SOUTHERN MEDICAL UNIVERSITY