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Method for separating whey protein from whey wastewater by foam separation method

A foam separation method and whey wastewater technology, applied in the field of waste water resource recycling, can solve the problems of low protein foam separation efficiency, limited whey protein reuse, unprocessed technology, etc., to achieve simple equipment, avoid waste, and avoid environmental pollution. pollution effect

Active Publication Date: 2018-10-12
QINGHAI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no foam separation method suitable for nomads to recover whey protein from yak whey wastewater, and most studies on protein foam separation only report initial concentration, pressure, gas velocity, temperature, pH value and The impact of single factors such as equipment improvement on protein foam separation, without process optimization, resulted in low efficiency of protein foam separation, which severely limited the reuse of whey protein in yak whey wastewater

Method used

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  • Method for separating whey protein from whey wastewater by foam separation method
  • Method for separating whey protein from whey wastewater by foam separation method
  • Method for separating whey protein from whey wastewater by foam separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Effects of whey protein concentration on the separation of yak whey protein foam

[0072] The yak whey wastewater was adjusted to pH 4.0-5.0 with 0.5mol / L NaOH solution and 0.5mol / L HCl solution, centrifuged at 10,000rpm for 10min at 4°C, repeated 3 times to remove fat, and the whey wastewater after treatment The medium fat content is less than 0.414g / L, and the whey protein content is 5.242g / L.

[0073] The mass concentration of whey protein in the defatted yak whey wastewater was diluted with deionized water to 70, 100, 130, 160, 190, 230, 260 μg / mL, and the diluted yak whey wastewater solution was diluted with 0.5mol / L The pH value of NaOH solution and 0.5mol / L HCl solution is adjusted to 4.3, and the diluted and pH adjusted yak whey wastewater solution is placed in a constant temperature water bath to keep the temperature constant until the temperature of the whey wastewater solution is 45°C.

[0074] The treated skim whey wastewater solution was injected into a ci...

Embodiment 2

[0080] The effect of temperature on the separation of yak whey protein foam

[0081] The method of Example 1 was used to separate the whey protein in the yak whey wastewater. The whey protein concentration was set to 130 μg / mL, and the temperature of the yak whey wastewater diluent and the foam separation column were adjusted to 20, 25, 30, 35, 40, 45, 50, and 55 °C, respectively, and other parameters and methods were implemented in the same way. example 1.

[0082] Table 2 Whey protein recovery and enrichment ratio at different temperatures

[0083] temperature ℃

[0084] Temperature significantly affects the surface tension of the solution, the stability of the foam and the rate of liquid reflux in the foam layer, thereby affecting the foam separation effect. As shown in Table 2, at 20-55 °C, with the increase of temperature, the recovery first increased and then decreased, and the enrichment ratio first decreased and then increased. At 35 °C, the recovery rate r...

Embodiment 3

[0086] Effect of pH value on separation effect of yak whey protein foam

[0087] The method of Example 1 was used to separate the whey protein in the yak whey wastewater. The whey protein concentration was set to 130 μg / mL, and the pH values ​​of the yak whey wastewater diluent were adjusted to 3.3, 3.8, 4.3, 4.8, 5.3, 5.8, and 6.3, respectively. Other parameters and methods were the same as those in Example 1.

[0088] Table 3 Whey protein recovery and enrichment ratio at different pH values

[0089]

[0090]

[0091] The pH value significantly affects the physicochemical properties of yak whey protein in aqueous solution, such as the net charge, solubility, interfacial tension and foam strength, etc., and has a significant impact on the foam separation performance of yak whey protein. As shown in Table 3, as the pH value increased from 3.3 to 6.3, the recovery first increased and then decreased, while the enrichment ratio gradually decreased. For the recovery rate, w...

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Abstract

The invention provides a method for separating whey protein from whey wastewater by a foam separation method. According to the method, by the study of multiple key factors, such as the whey protein concentration, gas velocity, temperature and pH, affecting the foam separation of yak whey protein, based on single-factor experiments, a process is optimized through a response surface method, and an optimal technological condition combination is obtained and comprises that the concentration of the whey protein is 100-160 [mu]g / mL, the gas velocity is 250-350 mL / min, the temperature is 40-50 DEG C,and the pH value is 3.5-4.5. Through verification tests, the recovery rate is 75-90% and the enrichment ratio is 8-10. The method is suitable for life characteristics of scattered nomads in Qinghai-Tibet Plateau, can effectively recover the whey protein from yak whey wastewater, also overcomes the difficulties that other technologies difficultly efficiently separate and enrich whey protein from low-concentration whey wastewater, and reduces the recovery cost of the whey protein.

Description

technical field [0001] The invention belongs to the field of waste water resource recovery and utilization, in particular to a method for separating whey protein from whey waste water by a foam separation method. Background technique [0002] Whey protein mainly contains α-lactalbumin (α-LA), β-lactoglobulin (β-LG), immunoglobulin (LG), serum albumin (SA), lactoferrin (LF) and lysozyme ( Lys), due to its high nutritional value and functional properties, has been widely used in food, health products, cosmetics and medicines. In recent years, with the improvement of human living standards and health awareness, the demand for whey protein has increased year by year. [0003] China has the largest number of yaks in the world, accounting for 92-95% of the world. Most of them live on the Qinghai-Tibet Plateau in Northwest China and play an indispensable role in the production and life of local herdsmen. Compared with ordinary cow's milk (or other mammals), yak milk contains more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/04C07K1/14C12N9/36C02F103/32
CPCC02F1/00C02F1/38C02F1/66C02F9/00C02F2103/327C07K1/14C12N9/2462C12Y302/01017
Inventor 张炜陈元涛刘龙辛小丽张文文付文才谭徐梅
Owner QINGHAI NORMAL UNIV
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