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Halobacillus trueperi S61 and application thereof

A technology of Bacillus and Terrier, applied in the field of microorganisms, can solve the problems of ryegrass plant growth promoting growth, etc., and achieve the effects of effective antagonism and control, wide source of raw materials and low cost

Active Publication Date: 2018-10-12
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there are only a handful of studies on Bacillus tertii. Xu Shuai et al.’s research on Tianjin seabed dredging mud showed that Bacillus tertii had no significant effect on the germination of ryegrass seeds, but had no significant effect on the growth of ryegrass plants. Has a growth-promoting effect

Method used

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  • Halobacillus trueperi S61 and application thereof
  • Halobacillus trueperi S61 and application thereof
  • Halobacillus trueperi S61 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Halobacterium tersonii S61

[0029] 1.1 Isolation and Purification of Bacillus tertii Halobacterium S61

[0030] 1) Water sample pretreatment: Under sterile conditions, mix the water sample evenly, filter it with a filter equipped with a filter membrane with a pore size of 0.22 μm, and enrich the bacteria on the filter membrane. When the amount of water sample used for enrichment reaches 30ml, remove the filter membrane and put it into a glass test tube filled with 3ml of sterile seawater for 10 -1 watery.

[0031] 2) Soil sample pre-treatment: Take an appropriate amount of soil sample, air-dry (about 20 days), and treat at 120°C for 1 hour. Weigh 10g of the treated soil sample, add 90ml of sterile seawater, put it into a sterilized Erlenmeyer flask equipped with glass balls, shake it fully for 30min, and draw the supernatant after standing still, the supernatant is 10 -1 Soil sample. the above 10 -1 Samples were serially d...

Embodiment 2

[0059] Antagonistic effect of embodiment 2 Halobacterium tertii S61 on pathogenic fungi

[0060] 1.1 Bacteria culture

[0061] Inoculate 4 strains of pathogenic fungi (Qing 9A-4-13, Qing 9A-5-2, Qing 9A-5-10, 65B-2-6) into PDA medium, and place them in a constant temperature incubator at 28°C for one week; Inoculate Bacillus tertii S61 on the modified medium of ATCC213 and culture it in a 37°C incubator for 1 day.

[0062] 1.2 Consistent training

[0063] Adopt " cross-hatching method ", the bacterium cake that the pathogenic fungus is perforated into the diameter 5mm is placed in distance center halophilic bacterium (colony diameter is 5mm) 2.5cm place, carries out confrontation culture in the petri dish of diameter 9cm. The results are shown in Table 3.

[0064] Table 3 The confrontation culture between s61 and 4 strains of potato dry rot pathogenic fungi

[0065]

[0066]

[0067] From Table 3 and figure 2 It can be seen that with the gradual increase of the con...

Embodiment 3

[0068] Example 3 Re-screening of potatoes in vivo

[0069] 1.1 Preparation of fermentation broth

[0070] The bacteria cultivated in Example 2 were inoculated into an Erlenmeyer flask filled with sterile water, placed in a constant temperature shaker at 37°C and 200 r / min, and cultured for 5 days with shaking for 5 days before use.

[0071] 1.2 Preparation of bacterial suspension

[0072] Inoculate the bacteria cultured in Example 2 into a Erlenmeyer flask filled with sterile water, place it in a constant temperature shaker at 37°C and 200 r / min for 2 hours, shake well and set aside.

[0073] 1.3 Test method

[0074] Prick two wounds with a diameter of 5 mm and a depth of 3 mm on the sterilized potato fruit with a puncher with a diameter of 5 mm (the potato fruit was soaked in 2% NaClO solution for 10 min, rinsed with tap water, and dried naturally). First inoculate 40 μl of pathogenic bacteria suspension, and then inoculate the same volume of Bacillus tertii S61 suspension...

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Abstract

The invention discloses Halobacillus trueperi S61 and application thereof. The bacterial strain is preserved at the Guangdong province China general microbiological culture collection center at the Guangdong province micro-organism research institution, addressed at the 5th floor, building 59, No.100, Martyr middle road, Guangzhou city, and the preservation number is GDMCC NO:60078. The strain anda suspension thereof or secondary metabolisms have the remarkable antagonism effect on diseases caused by fusarium, the materials are wide in source, the cost is low and the strain is safe to environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a halophilic bacterium derived from Chaerhan Salt Lake and its biocontrol application, in particular to a halobacterium teresii S61 and its application. Background technique [0002] Potatoes belong to the annual herbaceous plant of Solanaceae Solanaceae, which are rich in nutrients and contain high protein, vitamins and inorganic salts. Potato planting area continues to expand, and the occurrence of diseases is becoming more and more serious. Among them, Fusarium dry rot is one of the important diseases of potato during storage, which often leads to the decrease of potato tuber quality and the rate of commercial potato, which seriously affects its edible value and economic value, and becomes the main factor restricting the development of potato industry. At present, chemical fungicides are mainly used to control them, but chemical control measures not only cannot curb the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 沈硕李玮郭青云王舰
Owner QINGHAI UNIVERSITY
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