Unlock instant, AI-driven research and patent intelligence for your innovation.

A hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent

A technology of ultrasound contrast agent and polydopamine, which is applied in the field of medical drugs, can solve the problems of poor stability, complex process, poor degradability, etc., and achieve the effect of less time-consuming, simple synthesis process and good biocompatibility

Active Publication Date: 2021-05-18
CHONGQING MEDICAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to address the problems of poor stability of phospholipid liquid fluorocarbon nanoparticles as an ultrasound contrast agent, poor biodegradability of liquid fluorocarbon nanoparticles wrapped by existing photothermal materials, and complicated process for modifying active targeting ligands. Using docetaxel as a model drug, a new preparation method for hyaluronic acid-targeted phase-change nano-ultrasound contrast agent integrating photothermal and chemotherapy is provided

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent
  • A hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent
  • A hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of Phospholipid Liquid Fluorocarbon Nanoparticles

[0028] 20 mg soybean lecithin (SPC) and 2 mg drug were dissolved in 4 mL dichloromethane. The dichloromethane was quickly removed by evaporation under reduced pressure, forming a lipid film. After adding 2 mL of Tris buffer (pH 8.5), the lipid film was hydrated in an ultrasonic water bath for 5 minutes to obtain colostrum. The colostrum was sonicated for 20 min with an ultrasonic cell disruptor, and cooled in a refrigerator at 4° C. to obtain liposomes. Add 0.03mL of PFH to the liposome suspension, and then use an ultrasonic cell disruptor to sonicate for 5 minutes under ice-water bath conditions to obtain drug-loaded phospholipid liquid fluorocarbon nanoparticles.

[0029] 2. Preparation of hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent

[0030] Add 1ml PVP (50mg / ml) to 2mL phospholipid liquid fluorocarbon nanosuspension, stir and mix, the...

Embodiment 2

[0033] 1. In vitro stability of hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent and photothermal conversion effect of nanoparticles

[0034] Put 2ml of hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent and 2ml of phospholipid liquid fluorocarbon nano-particle ultrasonic contrast agent in a water bath at 37°C for 0, 30, 60, After 90 minutes, observe the phase transition as Figure 4 As shown, it shows that the physical stability of nanoparticles is enhanced after encapsulating polydopamine. At the same time, we recorded the temperature of 1ml of hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent with different concentrations (0, 50, 100, 250, 500, 1000 μg / ml) under laser irradiation. changes, such as Figure 5 As shown in A, use 2.5W / cm 2 After 500s of laser irradiation, the maximum temperature can r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasonic contrast agent. A hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent prepared by film-emulsification method and oxidative polymerization method has a particle size of about 248.08nm, and in vitro experiments show that it has good stability. Cytological experiments show that hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent has good biocompatibility. In vivo experiments show that hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent has good ultrasonic imaging effect. At the same time, its combined application of photothermal therapy and chemotherapy showed good tumor suppressive effect. The hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasound contrast agent prepared by the invention has simple and convenient technology, and the materials used have good biocompatibility and broad application prospects.

Description

technical field [0001] The invention relates to the field of medical medicine, in particular to a hyaluronic acid-targeted polydopamine-coated phase-change liquid fluorocarbon nano-ultrasonic contrast agent and a preparation method thereof. Background technique [0002] In clinical practice, disease diagnosis and treatment are two relatively independent processes. However, the separate use of contrast agents and drugs in the diagnosis process will lead to the superposition of side effects and increase the pain of medication for patients. Therefore, with the joint efforts of clinicians and scientific researchers, theranostics has gradually developed as a new concept. Theranostics is a means of combining drug therapy and diagnosis. In the case of a preliminary diagnosis, the diagnosis and treatment are realized in real time by using the theranostic agent to enhance the therapeutic effect and reduce the impact on the human body. Toxic and side effects, in order to achieve twi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K9/10A61K9/127A61K47/24A61K47/34A61K47/06A61K47/36A61K45/06A61K31/337A61K41/00A61P35/00
CPCA61K9/10A61K9/127A61K31/337A61K41/0052A61K45/06A61K47/06A61K47/24A61K47/34A61K47/36A61K49/221A61K49/226A61P35/00A61K2300/00
Inventor 张良珂牟崇艳
Owner CHONGQING MEDICAL UNIVERSITY