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A kind of fluorescent nanoparticle and preparation method thereof

A fluorescent nanoparticle and nanoparticle technology, applied in the field of fluorescent nanoparticles and their preparation, can solve the problems of intolerance, poor fluorescence stability, and difficult to apply fluorescent labeling fields, and achieve uniform scale, strong fluorescent signal, and good single-cell performance. dispersive effect

Active Publication Date: 2021-02-05
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] To sum up, the problems existing in the prior art are: affected by the environment of the solvent to be tested, fluorescent nanomaterials based on rare earth complexes are not tolerant to the environment to be tested, resulting in poor fluorescence stability, and are difficult to apply to the field of fluorescent labeling

Method used

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  • A kind of fluorescent nanoparticle and preparation method thereof
  • A kind of fluorescent nanoparticle and preparation method thereof
  • A kind of fluorescent nanoparticle and preparation method thereof

Examples

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Effect test

Embodiment 1

[0040] like figure 1 As shown, the simulation schematic diagram of the preparation method of fluorescent nanoparticles loaded with ZIF-8 rare earth complexes provided in the embodiment of the present invention, the preparation method of fluorescent nanoparticles loaded with ZIF-8 rare earth complexes provided by the present invention includes the following steps:

[0041] Step 1, pyridine two tetrazole (H 2 pytz) and triethylamine were dissolved in 1~2mL of methanol, added to a 10mL single-necked flask, mixed evenly, and then 1~4mL of terbium trifluoromethanesulfonate [Tb(OSO 2 CF 3 ) 3 ] Methanol solution in the system, stirred at room temperature for 24 hours, then vacuum-dried to remove the solvent to obtain a rare earth complex. The mass ratio of pyridine ditetrazolium, triethylamine and terbium trifluoromethanesulfonate is (43.3-129.2): (30.4-60.75): (30.3-121.2).

[0042] Step 2: Weigh 0.5 mg of the rare earth terbium complex and dissolve it in 200-300 μL of deionize...

experiment example 2

[0054] The unblocked stopper nanoparticles obtained in step 2 and the fluorescent nanoparticles loaded with ZIF-8 rare earth terbium complex obtained in step 4 were respectively dispersed in PBS buffer solution with pH = 7.4, and the particle concentration was configured to be 1 mg mL -1 solution, select the excitation light wavelength as 304nm, and detect the fluorescence emission intensity of the particles at 543nm every 20 minutes to verify the stability of the fluorescence. like Figure 8 It can be seen from the figure that when no stopper and TEOS are added, the fluorescence intensity of the particles continues to decay over time; when the stopper and TEOS are added at a ratio of 1:2, the stability of the fluorescent nanoparticles is enhanced, and the number of It does not attenuate for hours, indicating that the particles have good tolerance to the high salt concentration and complex ion environment in the physiological system.

Embodiment 2

[0056] Step 1, pyridine two tetrazole (H 2 pytz) and triethylamine were dissolved in 1~2mL of methanol, added to a 10mL single-necked flask, mixed evenly, and then 1~4mL of terbium trifluoromethanesulfonate [Tb(OSO 2 CF 3 ) 3 ] Methanol solution in the system, stirred at room temperature for 24 hours, then vacuum-dried to remove the solvent to obtain a rare earth complex. The mass ratio of pyridine ditetrazolium, triethylamine and terbium trifluoromethanesulfonate is (43.3-129.2): (30.4-60.75): (30.3-121.2). After stirring at room temperature for 24 hours, the rare earth complex was obtained.

[0057] Step 2: Weigh 0.1 mg of the rare earth terbium complex and dissolve it in 200-300 μL of deionized water, and add it into a 10 mL single-necked flask. After uniform dispersion, add 2-methylimidazole (HMIM) with a mass of 45-54 mg and 14-16.8 mg of hexahydrate and zinc nitrate (Zn(NO 3 ) 2 ·6H 2 O). Finally, add 400-600 μL of methanol into the system and mix well, and react...

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Abstract

The invention provides a fluorescent nanoparticle and a preparation method thereof, the method comprising: encapsulating a small molecule with fluorescent properties into the nanoparticle channel with a microporous structure; step 2: reacting the blocking molecule with the product obtained in step 1 The fluorescent nanoparticles of the present invention are obtained; wherein, the small molecules in step 1 are negatively charged, and the nanoparticles are positively charged; the blocking molecule is a molecular structure capable of blocking the pores of the nanoparticles. The average particle size of the obtained fluorescent nanoparticles is 100±5nm, and the specific surface area is 1429m 2 g ‑1 , the pore volume is 0.64cm 3 g ‑1 . The fluorescence intensity of the nanometer material has good stability, the quantum yield can reach 23.2%, and the fluorescence lifetime can reach 1.9ms, so it is an ideal material as a biological fluorescent probe.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and in particular relates to a fluorescent nano-particle and a preparation method thereof. Background technique [0002] With the vigorous development of life science and technology, the research of life science has penetrated into the level of cells and single molecules of organisms. Genomics, proteomics and metabolomics methods and sequencing technologies are booming, and people have discovered more and more biomolecules such as protein markers, nucleic acids, and polypeptides that reflect physiological and pathological conditions (Nature Methods, volume 11, page 1177 ,Year 2014). The labeling, staining and detection of cells or biomolecules has become an important part of biomedical research. Among biomarkers, the most concerned labeling method is fluorescent labeling, but its detection sensitivity mainly depends on the fluorescence intensity and stability of the label. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/06B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C09K11/025C09K11/06C09K2211/182C09K2211/188
Inventor 张吉喜陈凤
Owner CHONGQING UNIV
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