A color/fluorescence dual-signal visualization method for rapid detection of nitrite and its application

Abstract

The invention belongs to the technical field of food, and more specifically relates to a color / fluorescence double signal visible rapid nitrite detection method, and applications thereof. The color / fluorescence double signal visible rapid nitrite detection method comprises following steps: firstly, a carbon dot-neutral red composite system is prepared, wherein carbon dot synthesis and constructionof the carbon dot-neutral red composite system are carried out; a common filter paper is immersed in the carbon dot-neutral red composite system so as to obtain a carbon dot-neutral red composite system detection test paper; a sodium nitrite standard solution is prepared; a color standard colourimetric card and a fluorescence standard colourimetric card of nitrites are prepared; a software is adopted to extract the RGB values of each test paper, a matrix is constructed, and a multivariate linear regression model is constructed; and the content of nitrites in food to be detected is detected. The carbon dot-neutral red composite system possess color / fluorescence double response capacity on nitrites; detection operation steps are simple; the visible results are clear, visual, and accurate; the detection limit is lower; and it is promising for the color / fluorescence double signal visible rapid nitrite detection method to be used in large-scale food nitrite content detection.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a color / fluorescence double-signal visual rapid detection method and application of nitrite. Background technique [0002] Nitrite exists widely in the environment and is a common nitrogen-containing compound in nature. There are many sources of nitrite in food. For example, food is polluted by nitrite existing in nature through milk-producing animals and water. Vegetables may spontaneously produce nitrite during pickling or storage. In addition, meat products Nitrite is used as an additive during processing. Nitrite can react with secondary amines, tertiary amines, phthaloamines and amino acids in the human body to form carcinogenic N-nitrosamine compounds. Nitrosamines can enter the fetus through the placenta and have teratogenic effects on the fetus. Nitrite is a highly toxic substance. Adults can cause poisoning if they ingest 0.2-0.5g, and 3g can be fata...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defects existing in the prior art, such as: cumbersome operation, inability to meet the needs of daily detection, and being easily affected by other substances, etc., the present invention provides a color / fluorescence dual-signal visualized rapid detection method for nitrite

Images