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Hybrid peptide and application thereof in bacteriostasis

A hybrid peptide and strain technology, applied in hybrid peptides, applications, fusion polypeptides, etc., can solve the problems of gaps in antibacterial activity, high toxicity of antibacterial peptides, and high cost, and achieve good thermal stability, good application prospects, and bacteriostasis. activity-enhancing effect

Active Publication Date: 2018-10-23
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a certain gap in the antibacterial activity of antimicrobial peptides compared with antibiotics, and many antimicrobial peptides still have many problems such as strong toxicity, poor stability, and high cost.

Method used

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  • Hybrid peptide and application thereof in bacteriostasis
  • Hybrid peptide and application thereof in bacteriostasis
  • Hybrid peptide and application thereof in bacteriostasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1 Materials and methods

[0026] 1.1 Materials

[0027] 1.1.1 Plasmids and strains:

[0028] Pichia pastoris X-33 (His-Mut+) and expression vector pPICZα-A were purchased from Invitrogen; Escherichiacoli ATCC25922, Staphylococcus aureus ATCC25923, Staphylococcus aureusATCC6538, Salmonella enterica ATCC13076, Salmonella typhimurium ATCC14028, and Bacillus ATCC6 are preserved by our laboratory.

[0029] 1.1.2 Main reagents

[0030] Restriction enzymes XholⅠ, XbalⅠ, SacⅠ, T4 ligase (purchased from Takara (Dalian) Co., Ltd.); Zeocin, Tricine, agarose gel recovery kit, plasmid extraction kit, DNA molecular mass marker, small molecular mass Protein Maker (purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.); pMD18-T was preserved by our laboratory; other reagents were domestic analytically pure; polynucleotide and DNA sequencing was completed by BGI.

[0031] 1.2 Cloning of hybrid antimicrobial peptide Mel-Ran2Cb gene

[0032] The Mel-Ran2Cb hybrid ...

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Abstract

The invention discloses a hybrid peptide and an application thereof in bacteriostasis. The hybrid peptide has a broad-spectrum antimicrobial activity and can inhibit most gram-positive bacteria and gram-negative bacteria to certain degrees. Compared with original antibacterial peptides, the bacteriostatic activity of the hybrid antibacterial peptide on common pathogenic bacteria is obviously enhanced. The hybrid peptide has the characteristics of thermal stability and acid stability, and has a good application prospect in fields such as food preservation, disease control, animal feed additive,and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hybrid peptide and its application in antibacterial. Background technique [0002] Antimicrobial peptides are a class of polypeptide substances with broad-spectrum antibacterial activity. Some antimicrobial peptides can also activate the immune system, participate in cell signal transduction, regulate cell proliferation, and have anti-tumor, anti-virus, and anti-parasite functions. Antimicrobial peptides can destroy bacterial cell membranes, causing intracellular spillage to kill bacteria. The mechanism is different from that of antibiotics. Release, activate the immune system's recognition and presentation of antigens, and increase the differentiation, proliferation and response levels of immune cells. Because of its unique bactericidal mechanism and excellent physical and chemical properties, antimicrobial peptides have become a research hotspot for safe and green an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62A61K38/17A61P31/04A23K20/147A23K20/195A23L33/18
CPCA23K20/147A23K20/195A23L33/18A61K38/00A61P31/04C07K14/43572C07K14/463C07K2319/00
Inventor 张宏刚李莉
Owner GUANGZHOU GLAM BIOTECH
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