Preparation containing human joint fluid exosome, and using method thereof

Abstract

The invention discloses a preparation containing human joint fluid exosome. The preparation comprises 20-30% of the human joint fluid exosome, 15-18% of a polyethylene glycol solution, 7-12% of humanalbumin, 6.5-7.5% of methionine, 5.3-6.7% of propylene carbonate, 2.1-3.8% of vitamin C, 1.35-2.28% of tranexamic acid, 4.87-5.86% of a solvent, 5.66-7.32% of a phosphatidyl sarcosine complex, and thebalance of deionized water. The invention further discloses a using method of the preparation containing the human joint fluid exosome. The using method includes the following steps: separation and extraction of the human joint fluid exosome, production of the preparation, sterilization, resuscitation cryopreservation and injection use. The above formula design is reasonable, and the human jointfluid exosome is obtained by separation and extraction, and has a good treatment effect on joint diseases, so the exosome preparation is more safe than drug or surgical treatment, and reduces the trauma to the patient's body; and the exosome preparation can be preserved for a long time after being produced, is very convenient to use, has a good treatment effect, and facilitates promotion and application.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a preparation containing human synovial fluid exosomes and a method of use thereof. Background technique [0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Today, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. In 1983, exosomes were first produced in sheep reticulocytes It was discovered that Johnstone named it "exosome" in 1987; a variety of cells can secrete exosomes under normal and pathological conditions, which mainly come from the multivesicular bodies formed by the invagination of lysosomal particles in cells. The multivesicular outer membrane is released into the extracellular matrix after fusion with the cell membrane; all cultured cell types can secrete exosomes, and exosomes naturally exist in body fluids, exosomes exist in human synovial fluid exosomes, In the prior art, drugs or surger...

AI Technical Summary

Problems solved by technology

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Today, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. In 1983, exosomes were first produced in sheep reticulocytes It was discovered that Johnstone named it "exosome" in 1987; a variety of cells can secrete exosomes under normal and pathological conditions, which mainly come from the multivesicular bodies formed by the invagination of lysosomal particles in cells. The multivesicular outer membrane is released into the extracellular matrix after fusion with the cell membrane; all cultured cell types can secrete exosomes, and exosomes naturally exist in body fluids, exosomes exist in human synovial fluid exosomes, In the prior art, drugs or surgery are often used for the treatment of human arthropathy. The treatment effect is average, and the trauma to the patient's body is relatively large. Moreover, most of the current treatment methods for arthrosis can only relieve the disease, and cannot To fundamentally cure the disease, for this reason, we propose a preparation containing human joint fluid exosomes and its application method