Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for producing butanol by fermenting wastewater of preserved fruit factories by using clostridium acetobutylicum

A Clostridium acetobutylicum, butanol-producing technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of dehydrogenase activity reduction, competition with people for food, and large energy consumption. Achieve the effect of maintaining anaerobic state, reducing waste of nitrogen and waste of resources

Inactive Publication Date: 2018-11-16
FUZHOU UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Candied fruit pickling is a heavy pollution process, discharge thick black salt water wastewater, low pH value, high salinity, large molecular weight of organic pollutants, high osmotic pressure, salting out will reduce the activity of dehydrogenase; the increase of the density of wastewater will make the activity Sludge is easy to float and lose, which increases the difficulty of wastewater treatment
At present, our country adopts the method of sedimentation and electrolysis to treat the wastewater of the candied fruit factory, which consumes a lot of chemicals, energy, and is expensive
[0005] For a long time, butanol has been prepared by biological fermentation. Most of the raw materials are molasses and starch, which are expensive and compete with others for food. The cost of substrates contributes a lot to the overall cost of low-value-added fermentation products. This is one of the challenges of ABE fermentation. one

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing butanol by fermenting wastewater of preserved fruit factories by using clostridium acetobutylicum
  • Method for producing butanol by fermenting wastewater of preserved fruit factories by using clostridium acetobutylicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method of using Clostridium acetobutylicum to treat candied fruit factory waste water to ferment and produce butanol, the steps are as follows:

[0024] 1) Preparation of P2+PGY medium: raw material composition by weight: 0.5 parts of potassium dihydrogen phosphate, 0.5 parts of dipotassium hydrogen phosphate, 2.2 parts of ammonium acetate, MnSO 4 0.01 parts, MgSO 2 ·7H 2 O 0.2 parts, FeSO 4 ·7H 2 O 0.01 part, NaCl 0.01 part, p-aminobenzoic acid 0.001 part, vitamin B 1 0.001 part, 0.001 part of biotin, 1 part of yeast extract, 3 parts of peptone, 21.325 parts of 2-(N-morpholine) ethanesulfonic acid monohydrate, 1000 parts of deionized water;

[0025] 2) Preparation of Clostridium acetobutylicum seed solution: by weight, 5 parts of glucose were added to 100 parts of the P2+PGY medium prepared in step 1) to obtain a culture solution, which was sterilized by high-pressure steam The bacteria pot was sterilized at 121°C for 15 minutes and sterilized by ultraviolet l...

Embodiment 2

[0028] A method of using Clostridium acetobutylicum to treat candied fruit factory waste water to ferment and produce butanol, the steps are as follows:

[0029] 1) Preparation of P2+PGY medium: raw material composition by weight: 0.5 parts of potassium dihydrogen phosphate, 0.5 parts of dipotassium hydrogen phosphate, 2.2 parts of ammonium acetate, MnSO 4 0.01 parts, MgSO 2 ·7H 2 O 0.2 parts, FeSO 4 ·7H 2 O 0.01 part, NaCl 0.01 part, p-aminobenzoic acid 0.001 part, vitamin B 1 0.001 part, 0.001 part of biotin, 1 part of yeast extract, 3 parts of peptone, 21.325 parts of 2-(N-morpholine) ethanesulfonic acid monohydrate, 1000 parts of deionized water;

[0030] 2) Preparation of Clostridium acetobutylicum seed solution: by weight, 5 parts of glucose were added to 100 parts of the P2+PGY medium prepared in step 1) to obtain a culture solution, which was sterilized by high-pressure steam The bacteria pot was sterilized at 121°C for 15 minutes and sterilized by ultraviolet l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing butanol by fermenting preserved fruit factory wastewater by using clostridium acetobutylicum. According to the method, a clostridium acetobutylicum seedsolution is prepared, the preserved fruit factory wastewater which is diluted by ten times is fermented, hydrogen and carbon dioxide generated in the fermentation process are discharged through a gasexhaust device, the anaerobic state in the culture process is maintained, and fermentation liquor in a fermentation bottle is oscillated and uniformly mixed through a constant-temperature gas-bath oscillation box, so that the clostridium acetobutylicum can fully utilize reducing sugar in the fermentation liquor. Compared with an existing preserved fruit factory wastewater treatment method, the method has the advantages that the preserved fruit factory wastewater can be fully changed into wealth, the advantage of high sugar concentration in the preserved fruit factory wastewater is fully utilized, and therefore the pollution to the environment caused by aeration and the like is reduced, and the like. The method is expected to be used for turning waste into wealth, and the problem that the preserved fruit factory wastewater is difficult to process due to the facts that the the preserved fruit factory wastewater is low in pH value, high in salinity and large in molecular mass of organic pollutants can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of candied fruit factory wastewater treatment, and in particular relates to a method for using Clostridium acetobutylicum to ferment candied fruit factory wastewater to produce butanol. Background technique [0002] In recent years, great changes have taken place in energy production and consumption, the energy development mode has changed from extensive to intensive, the energy structure has changed from coal-based to diversified, the driving force of energy development has changed from traditional energy growth to new energy growth, and energy consumption has been effectively Control, further improve energy utilization efficiency, and achieve new results in energy saving and consumption reduction. With its long history of fermentation and relatively mature fermentation process, bio-butanol has come back to people's sight again. [0003] Butanol is not only an important organic solvent and chemical raw mat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/16C12N1/20C12R1/145
CPCC12N1/20C12P7/16Y02E50/10
Inventor 叶卓亮宋静怡卓淑敏宋鑫
Owner FUZHOU UNIV