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Dialysis membrane based on cross-linked lysozyme and application of dialysis membrane

A technology of lysozyme and dialysis membrane, which is applied in the field of dialysis membrane, can solve the problems such as the inability to remove medium and large molecular toxins, and achieve the effects of avoiding cumbersome steps and environmental pollution, good biocompatibility, and simple preparation methods

Active Publication Date: 2018-11-23
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional hemodialysis membrane cannot achieve the removal of medium and large molecule toxins.

Method used

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  • Dialysis membrane based on cross-linked lysozyme and application of dialysis membrane
  • Dialysis membrane based on cross-linked lysozyme and application of dialysis membrane
  • Dialysis membrane based on cross-linked lysozyme and application of dialysis membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Adjust 60 μL of 50 mmol / L tris(2-carboxyethyl)phosphine in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to pH 7.0 with NaOH, and then mix it with 60 μL of 2 mg / mL lysozyme’s 4-hydroxy After the ethylpiperazine ethanesulfonic acid buffer solution is mixed evenly, it is directly spread on the surface of a 18mm×18mm cover glass, incubated at room temperature for 2 hours, and a layer of lysozyme two-dimensional nano film is formed at the gas-liquid interface of the liquid on the cover glass; The lysozyme two-dimensional nanofilm at the gas-liquid interface was transferred to 1% glutaraldehyde aqueous solution, and cross-linked at room temperature for 2 hours to obtain a cross-linked lysozyme nanofilm.

[0025] The above-mentioned cross-linked lysozyme nano-film is adhered to a PET nuclear pore membrane with a membrane diameter of 25 mm, a thickness of 12 μm, and a pore size of 10 μm to obtain a dense skin layer with a cross-linked lysozyme nano-film and a PET ...

Embodiment 2

[0027] In this embodiment, the 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of 2mg / mL lysozyme in Example 1 is replaced with an equal volume of 4mg / mL lysozyme 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, Other steps were the same as in Example 1 to obtain a dialysis membrane.

Embodiment 3

[0029] In this embodiment, the 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of 2mg / mL lysozyme in Example 1 is replaced with an equal volume of 6mg / mL lysozyme 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, Other steps were the same as in Example 1 to obtain a dialysis membrane.

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Abstract

The invention discloses a dialysis membrane based on the cross-linked lysozyme and application of the dialysis membrane. The dialysis membrane is a two-dimensional nano film formed by the phase transformation of the lysozyme which is adhered to a PET nuclear track membrane after being cross-linked by using glutaraldehyde, and the dialysis membrane adopting the cross-linked lysozyme nano film as acompact wrapper layer and the PET nuclear track membrane as a dialysis membrane of a supporting layer. The dialysis membrane is easy to prepare in a large scale, also has the characteristics of low cost, low energy consumption, environmental protection and the like at the same time, avoids the complicated step and environmental pollution problem in the traditional polymer film synthetic process, is better in biological compatibility, capable of selectively separating different molecules, capable of completely intercepting the molecules with the size of 3.2nm or more, is higher in diffusion rate for the small molecules, and capable of being used as a blood purifying film to remove urea, creatinine and medium-large-molecular toxins and indoxyl-sulfate, and is a relatively ideal dialysis membrane material.

Description

technical field [0001] The invention relates to a dialysis membrane, in particular to a simple, green, mild and controllable dialysis membrane and the application of the dialysis membrane. Background technique [0002] Due to the characteristics of high separation efficiency, no secondary pollution and simple operation, membrane separation technology has been widely used in petrochemical, sewage treatment, medical and health, food processing and other fields. Membrane material is one of the core contents of membrane separation technology. At present, commonly used membrane materials are mainly organic synthetic polymer materials. However, the preparation method of polymer membrane is complicated, and it is usually easy to absorb organic substances (such as proteins, colloids and microorganisms) in the raw material solution. etc.); when in contact with blood, the non-specific adsorption of proteins on the membrane surface can also cause negative effects such as coagulation, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/44B01D69/10B01D67/00
CPCB01D67/0006B01D69/10B01D71/44B01D2325/04B01D61/243B01D71/48B01D67/0034B01D69/125B01D67/0093B01D2323/38B01D2323/40B01D2323/30B01D69/02B01D69/144B01D67/00931B01D71/441B01D69/1251B01D69/1071B01D2325/0283
Inventor 杨鹏杨发翠
Owner SHAANXI NORMAL UNIV
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