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A kind of lyophilized powder for detection of p-hydroxyphenylalanine and its preparation method and application

A technology of hydroxyphenylalanine and freeze-dried powder, which is applied in the direction of measuring devices, analyzing materials through chemical reactions, and analyzing materials through observing the impact on chemical indicators, which can solve transportation inconvenience, environmental hazards, To solve the problem of excessive use of waste, to achieve the effect of easy storage and transportation, easy use, and environmental friendliness

Active Publication Date: 2020-05-22
SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: 1) the reagent adopts the coordination principle of amino acids and metal ions, and the uric acid in urine, etc., produces great interference at high concentrations, resulting in false negative results; 2) the detection methods all contain mercury ions, mercury The toxicity not only makes the preparation process have a certain safety hazard, in addition, the detection of heavy metal ions such as mercury and nickel in the waste liquid will also cause harm to the environment, so production and recycling require special treatment, which increases the cost of use; 3) strong acids such as sulfuric acid and nitric acid There are also many safety problems in the preparation process and use process; 4) At present, the detection kits are all packaged in ampoule liquid, and then mixed with urine for detection. It's not convenient

Method used

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  • A kind of lyophilized powder for detection of p-hydroxyphenylalanine and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Weigh sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ·H 2 O): 1.08g and disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O): 4.37g was mixed with water and made to 100mL to obtain a phosphate buffer solution with a pH of 6.8;

[0026] 2) Weigh 50KU tyrosinase dry powder and add it;

[0027] 3) Then add 0.1g 4-aminoantipyrine;

[0028] 4) Then add 1.1g bovine serum albumin, 2.8g sucrose and 1.1g Triton X-100 to obtain a mixed solution, the concentration of sodium dihydrogen phosphate monohydrate in the mixed solution is 10.8mg / mL, hydrogen phosphate dodecahydrate The concentration of disodium is 43.7mg / mL, the concentration of tyrosinase is 500U / mL, the concentration of 4-aminoantipyrine is 1mg / mL, the concentration of sucrose is 0.028g / mL, and the concentration of albumin is 0.011g / mL and the concentration of Triton X-100 is 0.011g / mL;

[0029] 5) Divide the mixed solution into ampoules in an amount of 100 μL per bottle, then freeze the ampou...

Embodiment 2

[0031] 1) Weigh sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ·H 2 O): 1.08g and disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O): 4.37g was mixed with water and made to 100mL to obtain a phosphate buffer solution with a pH of 6.8;

[0032] 2) Weigh 50KU tyrosinase dry powder and add it;

[0033] 3) Then add 0.1g 4-aminoantipyrine;

[0034] 4) Then add 200 μl of bright green aqueous solution (0.1% concentration):

[0035] 5) Then add 1.1g bovine serum albumin, 2.8g sucrose and 1.1g Triton X-100 to obtain a mixed solution, the concentration of sodium dihydrogen phosphate monohydrate in the mixed solution is 10.8mg / mL, hydrogen phosphate dodecahydrate The concentration of disodium is 43.7mg / mL, the concentration of tyrosinase is 500U / mL, the concentration of 4-aminoantipyrine is 1mg / mL, the concentration of sucrose is 0.028g / mL, and the concentration of albumin is 0.011g / mL and the concentration of Triton X-100 is 0.011g / mL;

[0036] 6) Divide the m...

Embodiment 3

[0037] Embodiment 3 effect verification test

[0038] 3.1 Color change experiment

[0039] The test sample is a standard solution of p-hydroxyphenylalanine with a concentration of 0mg / L, 120mg / L, 240mg / L, and 480mg / L.

[0040] Add 500 μL of the test sample into the ampoule of implementation 1 or 2, and wait for 5 minutes for the liquid to change color. The sample color development method of Example 3 is mixed with the test sample, and the color change results are shown in Table 1.

[0041] Table 1

[0042]

[0043] It can be found from Table 1 that after the lyophilized powder provided by the present invention is added to the test sample containing p-hydroxyphenylalanine, the sample changes color, the higher the content, the higher the degree of discoloration, and the bright green lyophilized powder is added. The color changes are richer and more distinctive.

[0044] Preparation method of standard color card

[0045] Add a plurality of standard solutions to the ampoul...

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PUM

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Abstract

Disclosed is a lyophilized powder for detecting p-hydroxyphenylalanine, a preparation method, and an application thereof. The lyophilized powder contains tyrosinase and 4-aminoantipyrine. The preparation method includes preparing a buffer, dissolving other components in the buffer, lyophilizing the mixed solution at -80°C, and drying the same with a freeze dryer. The lyophilized powder can be used to prepare a testing kit for detecting p-hydroxyphenylalanine in urine. The technical solution uses tyrosinase to detect the concentration of p-hydroxyphenylalanine, and successfully develops the same to obtain a lyophilized powder product for urine testing. The test reagent components are environmentally friendly, free of heavy metal ions such as mercury or nickel, and no environmental pollution will be caused after use. In addition, the lyophilized powder is convenient for storage and transportation, and is also convenient to use.

Description

technical field [0001] The invention belongs to the technical field of detection reagents, and in particular relates to a freeze-dried powder for detection of p-hydroxyphenylalanine, a preparation method and an application. Background technique [0002] Early diagnosis and early treatment of tumors are the key to improving the cure rate of tumors. The commonly used diagnostic methods in clinical practice include chest X-ray, B-ultrasound, CT, and nuclear magnetic resonance, etc., which are often accompanied by puncture, blood drawing, etc., which aggravate the pain of the patient and may even cause cross-infection, and are expensive. More importantly, through these The tumors that can be detected by means are generally in the middle and advanced stages, and the cure rate is greatly reduced. [0003] Abnormal nucleotide metabolism of cancer cells produces a monohydric phenol metabolite, in which the content of p-hydroxyphenylalanine is much higher than that of normal people,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 刘东
Owner SHENZHEN HUACHUANG BIO PHARM TECH CO LTD