Use of a kind of kappa opioid receptor agonist in preparation of medicine for treating pruritus caused by farnesoid x receptor agonist
A receptor agonist, kappa opioid technology, applied in the field of itching drugs, can solve the problems of undisclosed itching, undisclosed kappa opioid receptor agonists combined use, etc.
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Embodiment 1
[0048] Embodiment 1: the preparation of formula (II) and formula (III) compound
[0049] The monitoring of the reaction progress in the embodiment adopts thin-layer chromatography (TLC), and the system of developing agent used in the reaction has: A: dichloromethane and methanol system, B: n-hexane and ethyl acetate system, C: petroleum ether And ethyl acetate system, D: acetone, the volume ratio of the solvent is adjusted according to the polarity of the compound.
[0050] Formula (II) compound: the following formula 1, (R)-N-((R)-6-amino-1-(4-(3-methylureido)piperidin-1-yl)-1-oxygen Hexan-2-yl)-2-((R)-2-((R)-2-amino-3-phenylpropanylamino)-3-phenylpropanylamino)-4-methylpentane Amide
[0051]
[0052]
[0053] first step
[0054] tert-butyl 4-((phenoxycarbonyl)amino)piperidine-1-carboxylate 1b
[0055] 4-Aminopiperidine-1-carboxylic acid tert-butyl ester 1a (0.5g, 2.5mmol), pyridine (0.22g, 2.75mmol) was dissolved in 15mL tetrahydrofuran, and phenyl chloroformate (0...
Embodiment 2
[0106] Example 2: Study on the therapeutic effect of κ opioid receptor agonists on obeticholic acid-induced pruritus
[0107] laboratory apparatus:
[0108] Table 1. Main instrument information
[0109]
[0110] Preparation of the test solution:
[0111] (1) 20mM acetic acid solution: Take 1.13mL of glacial acetic acid in a suitable container, add pure water to dilute to 1000mL.
[0112](2) 20mM sodium acetate buffer solution: Weigh 1.64g of sodium acetate into a suitable container, add 1000mL of pure water, stir until completely dissolved, adjust the pH to 4.5-4.6 with 20mM acetic acid solution, and stir evenly.
[0113] (3) Obeticholic acid solution: Weigh an appropriate amount of obeticholic acid and grind it, dissolve it in ultrapure water to prepare an administration solution with a concentration of 1.0 mg / mL.
[0114] (4) Compound solution of formula (III): Weigh an appropriate amount of compound of formula (III), and prepare administration solutions with concentra...
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