Codfish peptide and enzymolytic extraction method thereof

An extraction method and cod peptide technology, which are applied in the field of cod peptide and its enzymatic hydrolysis extraction, can solve problems such as waste of deep-sea biological resources, and achieve the effects of promoting bone formation, enhancing osteogenesis and improving bone metabolism.

Active Publication Date: 2018-11-30
重庆申高生化制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most cod skins are treated as crude protein fee

Method used

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  • Codfish peptide and enzymolytic extraction method thereof

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Experimental program
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Effect test

preparation example Construction

[0030] Preparation of sample solution: weigh 10.00 g of cod fish peptide, add 300 mL of deionized water, mix well, and ultrasonically treat in a water bath at 50° C. for 20 min. The ultrasonic power of the ultrasonic treatment is 300 W and the ultrasonic frequency is 35 kHz to obtain a sample solution.

[0031] Hydroxyl radical (·OH) scavenging experiment: using Fenton system o-phenanthroline-Fe 2+ Determination by oxidation method, take 1.5mL 1mmol / L o-phenanthroline into a test tube, add 4.5mL 100mmol / L pH7.4 phosphate buffer solution, mix well, add 1mL 1mmol / L FeSO 4 Mix the solution immediately, add 1mL sample solution, and then add 1mL 0.1% H 2 O 2 The aqueous solution started the reaction, incubated at 37°C for 60 minutes, and measured the absorbance at 509nm. At the same time set up the damage tube (add 1mL distilled water instead of the sample solution, 1mL mass fraction of 0.1% H 2 O 2 The aqueous solution starts the reaction) and the undamaged tube (add 2mL distilled wate...

Embodiment 1

[0045] The enzymatic extraction method of cod peptides includes the following steps:

[0046] S1. Choose fresh cod skin and cut into pieces of fish skin with a size of 2cm×2cm;

[0047] S2. Put the fish skin fragments into the reaction pot, add water, where the amount of water added is 2 times the weight of the fish skin fragments, then adjust the pH to 8.5 with a 0.1 mol / L sodium hydroxide aqueous solution, and then add lipase, so In step S2, the amount of lipase added is 2‰ of the weight of the fish skin fragments. The enzymatic hydrolysis is carried out at a temperature of 38°C for 60 minutes, filtered with a 300-mesh filter cloth, and the filter cake is washed 3 times with water. 30% of the weight of the cake to obtain defatted fish skin fragments;

[0048] S3. Mix the defatted fish skin fragments with water at a mass ratio of 1:3 to obtain a mixture, add papain with a weight of 0.3‰ of the mixture to the mixture, and carry out enzymatic hydrolysis at a temperature of 58°C for 1...

Embodiment 2

[0054] The enzymatic extraction method of cod peptides includes the following steps:

[0055] S1. Choose fresh cod skin and cut into pieces of fish skin with a size of 2cm×2cm;

[0056] S2. Put the fish skin fragments into the reaction pot, add water, where the amount of water added is 2 times the weight of the fish skin fragments, then adjust the pH to 8.5 with a 0.1 mol / L sodium hydroxide aqueous solution, and then add lipase, so In step S2, the amount of lipase added is 2‰ of the weight of the fish skin fragments. The enzymatic hydrolysis is carried out at a temperature of 38°C for 60 minutes, filtered with a 300-mesh filter cloth, and the filter cake is washed 3 times with water. 30% of the weight of the cake to obtain defatted fish skin fragments;

[0057] S3. Mix the defatted fish skin fragments, hydroxypropyl-β-cyclodextrin and water at a mass ratio of 1:0.01:3, then use colloid grinding to obtain fish skin slurry, and add fish to the fish skin slurry Papain with a weight of...

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Abstract

The invention discloses codfish peptide and an enzymolytic extraction method thereof. The enzymolytic extraction method comprises the following steps of: (S1) selecting fresh codfish skin and cuttinginto codfish skin chips; (S2) adopting lipase for enzymolysis; (S3) adopting papain for enzymolysis; (S4) mixing active carbon and enzymolysis liquid, and removing fishy smell; (S5) carrying out ultrafiltration on decoloring liquid by using an ultrafiltration membrane with the cutoff molecular weight being 50-140kD, and carrying out vacuum freezing and drying to obtain the codfish peptide. The codfish peptide and the enzymolytic extraction method disclosed by the invention have the beneficial effects that the codfish skin is used as a main raw material, so that the safety and the naturality ofthe source are ensured; the prepared codfish peptide has higher activity and digestible absorptivity, has multiple functions of metabolism and physiological regulation of a human body, is easily digested and absorbed, has the effects of promoting immunity, regulating hormone, resisting bacteria and viruses, reducing blood pressure and blood fat, lubricating joints, heeling wounds and resisting aging and the like, and also has the functions of improving the elasticity and activity of the skin of the human body, enhancing the bone strength, improving bone metabolism, enhancing the bone-formingaction, preventing the bone diseases such as osteoporosis, osteoproliferation and swelling and pain of bone joints.

Description

Technical field [0001] The invention relates to the field of food technology, in particular to a cod peptide and an enzymatic extraction method thereof. Background technique [0002] Collagen is the most widely distributed and most abundant protein in animals, and it is widely present in connective tissues such as animal skin, bone, tendon and membrane. Collagen has a triple helix structure and retains biological activity. The triple helix structure is completely loosened and becomes 3 free peptide chains, which are degraded into polydisperse peptides. Therefore, collagen is a mixture of polypeptides. Studies have found that polypeptides are involved in various physiological changes and biochemical reactions in life activities. [0003] The protein is hydrolyzed to obtain a polypeptide, which changes the structure of the protein, and exposes the active functional groups in the hydrophobic region. With the cleavage of the peptide bond, small molecular protein peptides and amino aci...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23J3/34
CPCA23J1/04A23J3/341A23V2002/00A23V2250/55A23V2300/38
Inventor 廖代平刘新旗
Owner 重庆申高生化制药股份有限公司
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