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Method for rapidly extracting astragaloside from Astragalus membranaceus

A technique for astragaloside IV and astragalus, which is applied in the field of rapidly extracting astragaloside IV from astragalus, and achieves the effects of eliminating alkaline treatment, preliminary extraction time and rapid extraction.

Active Publication Date: 2018-12-04
安徽弘腾药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of method for extracting astragaloside IV from Radix Astragali rapidly in view of existing problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for rapidly extracting astragaloside IV from Astragalus, comprising the following:

[0019] (1) Dried Astragalus membranaceus at a temperature of 70°C until the water content is lower than 3%, pulverized through a 40-mesh sieve, and then pulverized through a superfine pulverizer to pass through a 200-mesh sieve to obtain superfine powder for use;

[0020] (2) Pack and seal the above-mentioned superfine powder material, and then use a radiation dose of 70kGy 60 Irradiation treatment of Co-γ-ray irradiation device;

[0021] (3) Quick-freeze the obtained radiant material in a quick-freezing equipment at a temperature of -18°C for 2 hours, and then extract it in an ethanol solution with a mass concentration of 70% at a temperature of 45°C. After extraction for 1.5 hours, centrifuge and collect Serum;

[0022] (4) Adjust the pH of the supernatant obtained above to 7.4, sequentially use petroleum ether, ethyl acetate, and n-butanol for gradient extraction, then rec...

Embodiment 2

[0031] A method for rapidly extracting astragaloside IV from Astragalus, comprising the following:

[0032] (1) Dried astragalus at a temperature of 65°C until the water content is lower than 3%, crushed through a 40-mesh sieve, and then pulverized through a superfine pulverizer to pass through a 200-mesh sieve to obtain a superfine powder for use;

[0033] (2) Pack and seal the above-mentioned superfine powder material, and then use a radiation dose of 80kGy 60 Irradiation treatment of Co-γ-ray irradiation device;

[0034] (3) Quick-freeze the obtained radiant material in a quick-freezing equipment at a temperature of -18°C for 2 hours. After completion, extract it in an ethanol solution with a mass concentration of 65%. The extraction temperature is 48°C. After extraction for 2 hours, centrifuge and collect. Serum;

[0035] (4) Adjust the pH of the supernatant obtained above to 7.6, sequentially use petroleum ether, ethyl acetate, and n-butanol for gradient extraction, the...

Embodiment 3

[0044] A method for rapidly extracting astragaloside IV from Astragalus, comprising the following:

[0045] (1) Dried Astragalus membranaceus at a temperature of 75°C until the water content is lower than 3%, pulverized through a 40-mesh sieve, and then pulverized through a superfine pulverizer to pass through a 200-mesh sieve to obtain a superfine powder for use;

[0046] (2) Pack and seal the above-mentioned superfine powder material, and then use a radiation dose of 60kGy 60 Irradiation treatment of Co-γ-ray irradiation device;

[0047] (3) Quick-freeze the obtained radiant material in a quick-freezing equipment at a temperature of -18°C for 2 hours. After completion, extract it in an ethanol solution with a mass concentration of 75%. The extraction temperature is 42°C. After extraction for 1 hour, centrifuge and collect. Serum;

[0048](4) Adjust the pH of the supernatant obtained above to 7.2, sequentially use petroleum ether, ethyl acetate, and n-butanol for gradient e...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to a method for rapidly extracting astragaloside from Astragalus membranaceus. The method comprises the steps of preparation of an ultrafine powder, irradiation treatment by a [60]Co-gamma ray irradiation device, quick freezing treatment and extraction in an ethanol solution, gradient extraction, purification, and recrystallization. Compared with the prior art, the method has the following advantages: through the steps of ultrafine pulverization, treatment by the [60]Co-gamma ray irradiation device and quickfreezing combined with pretreatment, fiber inside the Astragalus membranaceus and cell wall structures can be effectively changed, and further polysaccharide and a part of organic components in the Astragalus membranaceus can be effectively removed, and through combination of gradient extraction and purification, the purity of the obtained astragaloside reaches 98.5% or more, alkaline treatment and the preliminary extraction time are saved, and the purpose of rapid extraction of astragaloside is rapidly achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for rapidly extracting astragaloside IV from Radix Astragali. Background technique [0002] Astragalus is a kind of leguminous plant, which is widely planted in my country as a raw material of traditional Chinese patent medicine. It is used as a tonic in traditional medicine. It has the characteristics of sweet taste and slightly warm nature. It shows that the main component of Astragalus is astragaloside IV, its clinical curative effect can improve cardiopulmonary function, expand blood vessels, lower blood pressure, and can protect liver and liver, prevent liver glycogen reduction, and has a good effect on chronic hepatitis; astragaloside IV also It can induce interferon and mobilize the body's immune function, inhibit virus replication and tumor growth, enhance the body's immunity, increase human energy, resist fatigue, and inhibit osteoclasts. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J53/00
CPCC07J53/001
Inventor 张家勤
Owner 安徽弘腾药业有限公司